FastQCFastQC Report
Thu 26 May 2016
SRR1546611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546611_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2013424
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94130.467512059059592No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC254.4440374E-544.039
TCAATCG207.8578945E-444.037
AATCGCG850.044.01
CGTTTTT84750.041.4300881
CGACAAT2400.038.520
GCGATAA2350.038.382989
TACGGGA8500.038.047064
AACGGGA9500.037.5157934
AGGGCGA17950.037.2590526
ACGGGAT11100.037.063065
CGACGGT1250.036.9628
CGGGATT11050.036.832586
CGTTAGG3650.036.7671242
AGGGATT45900.036.5228776
GGGCGAT40500.036.449387
TAGGGAT36550.035.8741465
TAAGGGA28350.035.7742544
ATAGGGA31350.035.6491244
GTAGGGT8300.035.2530144
AGGGATC25850.035.2340436
Produced by FastQC (version 0.11.5)