##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546611_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2013424
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.902876890312225	31.0	31.0	33.0	30.0	34.0
2	31.276021344734144	31.0	31.0	34.0	30.0	34.0
3	31.43765992657284	31.0	31.0	34.0	30.0	34.0
4	35.23459986570141	37.0	35.0	37.0	33.0	37.0
5	35.0348739262073	37.0	35.0	37.0	32.0	37.0
6	35.10436996876962	37.0	35.0	37.0	32.0	37.0
7	35.628489577952784	36.0	35.0	37.0	35.0	37.0
8	35.660820075652225	37.0	35.0	37.0	35.0	37.0
9	37.415045216506805	39.0	37.0	39.0	35.0	39.0
10	36.6350113041267	39.0	35.0	39.0	32.0	39.0
11	36.25968598765089	38.0	35.0	39.0	31.0	39.0
12	35.85906396268248	37.0	35.0	39.0	31.0	39.0
13	35.754742667217634	37.0	35.0	39.0	30.0	39.0
14	36.69361694307806	38.0	35.0	40.0	31.0	41.0
15	36.85367662250972	38.0	35.0	40.0	31.0	41.0
16	36.87010435953878	38.0	35.0	40.0	31.0	41.0
17	36.76839403920883	38.0	35.0	40.0	31.0	41.0
18	36.67833600870954	38.0	35.0	40.0	31.0	41.0
19	36.65319823345703	38.0	35.0	40.0	31.0	41.0
20	36.52560762164353	38.0	35.0	40.0	30.0	41.0
21	36.42140254611051	38.0	35.0	40.0	30.0	41.0
22	36.35534393153156	38.0	35.0	40.0	30.0	41.0
23	36.1626716478993	38.0	34.0	40.0	30.0	41.0
24	36.103105456178135	38.0	34.0	40.0	30.0	41.0
25	35.93495259816114	38.0	34.0	40.0	29.0	41.0
26	35.82642801516223	38.0	34.0	40.0	29.0	41.0
27	35.63847505542797	38.0	34.0	40.0	29.0	41.0
28	35.58169118874117	38.0	34.0	40.0	29.0	41.0
29	35.43838456281439	37.0	34.0	40.0	27.0	41.0
30	35.413709680623654	38.0	34.0	40.0	27.0	41.0
31	35.36275022051987	38.0	34.0	40.0	27.0	41.0
32	35.17422957111865	38.0	34.0	40.0	26.0	41.0
33	34.96504114384253	38.0	33.0	40.0	25.0	41.0
34	34.86845542717282	38.0	34.0	40.0	25.0	41.0
35	34.657301691049675	37.0	33.0	40.0	24.0	41.0
36	34.47818194279993	37.0	33.0	40.0	24.0	41.0
37	34.55403730163145	37.0	33.0	40.0	24.0	41.0
38	34.504893653795726	37.0	33.0	40.0	24.0	41.0
39	34.38745192269487	37.0	33.0	40.0	23.0	41.0
40	34.37135794547	37.0	33.0	40.0	23.0	41.0
41	34.38617946344138	37.0	33.0	40.0	23.0	41.0
42	34.15393379635884	37.0	33.0	40.0	23.0	41.0
43	34.20530946288512	37.0	33.0	40.0	23.0	41.0
44	34.000888039479015	37.0	33.0	40.0	23.0	41.0
45	33.827246521348705	37.0	33.0	40.0	22.0	41.0
46	33.90421441286088	37.0	33.0	40.0	23.0	41.0
47	33.74281075421769	36.0	32.0	40.0	22.0	41.0
48	33.787610061268765	36.0	32.0	40.0	22.0	41.0
49	33.69622990487846	36.0	32.0	40.0	22.0	41.0
50	33.22665270703041	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	19.0
15	69.0
16	200.0
17	557.0
18	1226.0
19	2465.0
20	4304.0
21	6713.0
22	10014.0
23	14683.0
24	20233.0
25	28001.0
26	36636.0
27	43294.0
28	49517.0
29	56136.0
30	65678.0
31	77846.0
32	93649.0
33	115777.0
34	160881.0
35	196009.0
36	171812.0
37	209622.0
38	280595.0
39	367479.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.43935504891171	24.241093778558316	26.882961561995884	15.436589610534096
2	30.829770579867926	26.232775610104973	28.940402021630813	13.997051788396284
3	27.804377021432146	26.19492963230795	32.09944850165688	13.901244844603026
4	24.112854520458683	29.019769308402005	32.13754281264155	14.729833358497762
5	21.676060283377968	33.20631918562608	31.182552706235743	13.935067824760209
6	20.468962324875438	41.43752135665414	27.82657800840757	10.266938310062859
7	88.89538418137461	3.206676785416286	6.619867449677763	1.2780715835313377
8	89.34367525171052	2.5490408378960416	6.333340617773504	1.773943292619935
9	83.63464426767536	5.35520585827923	8.389986411207973	2.620163462837435
10	38.66279531782675	35.09404874482473	14.632586082216164	11.610569855132352
11	29.067896280167517	25.56982533236914	29.816074507902957	15.546203879560391
12	28.626210872622952	23.428597255222943	29.946250764866218	17.998941107287884
13	22.286363925333163	28.933051359276536	31.053618115210707	17.726966600179594
14	19.350519314362003	31.04447945390539	30.900694538259206	18.70430669347341
15	18.2433009639301	25.42842441532434	38.3103111912841	18.017963429461457
16	20.75524082359205	23.65487845580464	37.026577611074465	18.563303109528842
17	21.069233306049796	23.882003989224327	30.83647557593433	24.21228712879155
18	21.636624973180016	25.249972186682985	33.42932238813086	19.684080452006132
19	24.68670285046766	26.295454906666453	29.369273436692918	19.648568806172968
20	26.419969166951425	26.019159402093152	28.894063048816342	18.666808382139084
21	23.894718648431727	27.513578858700406	29.61954362320107	18.972158869666796
22	22.870940249048388	25.095757277155727	29.449286389751983	22.5840160840439
23	20.976952693521085	28.03026088891361	29.530888675211976	21.461897742353326
24	20.94188804742568	25.54384968094152	34.98349080968539	18.53077146194741
25	20.259766447603685	26.504750117213266	32.55970923163725	20.6757742035458
26	19.908027320623972	29.72975389187772	29.445362725387202	20.916856062111112
27	19.790764389418225	29.830626832698925	29.84448382456949	20.53412495331336
28	18.73226901040218	28.297616398731712	33.30912912531092	19.660985465555196
29	19.70787077138248	25.69379325964129	33.09203625267207	21.506299716304166
30	20.485848981635264	28.194905792321933	31.046962785781833	20.27228244026097
31	23.69719443097927	26.931038867123863	28.942935020144787	20.42883168175208
32	24.763636471999938	26.66507402315657	28.568100906714132	20.003188598129356
33	23.802537369178076	27.875797646198713	27.81560168151368	20.506063303109528
34	20.364165719689446	28.02941655607562	29.216349859741413	22.39006786449352
35	20.32363774346586	27.892535303045957	30.556802740009058	21.22702421347913
36	23.818033360087096	28.17791980228705	27.415586582855873	20.588460254769984
37	20.787226138160666	31.05108511669673	28.458784637513013	19.70290410762959
38	21.136084600163702	30.434871144875597	27.970064924228577	20.458979330732127
39	21.122227608293137	30.404624162620493	28.11062150843538	20.36252672065099
40	23.242297697852017	27.659002773385037	28.117326504501783	20.98137302426116
41	19.215128060458206	27.777259037341366	29.228518185935997	23.779094716264435
42	21.19652889803638	27.51318152560017	27.780288702230628	23.51000087413282
43	20.973376695619006	27.183395052408237	28.858203736520476	22.985024515452285
44	20.449443336326574	28.357266030403927	29.6532672700832	21.540023363186293
45	19.853394019342176	30.774143945835554	27.578741487138323	21.793720547683947
46	21.14517359483149	29.549215664460142	28.312069390252624	20.99354135045574
47	20.48738864739866	28.267170749926496	28.982022663880038	22.26341793879481
48	21.015643004156104	26.884749560946926	30.76058495379016	21.339022481106813
49	20.99806101447087	27.014528484809958	30.556455073546356	21.430955427172815
50	20.308936418757302	29.50158535906992	29.292290148523115	20.897188073649666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1656.0
1	2012.5
2	2369.0
3	10409.0
4	18449.0
5	13974.0
6	9499.0
7	9855.5
8	10212.0
9	10740.5
10	11269.0
11	11441.0
12	11613.0
13	11574.5
14	11536.0
15	11196.0
16	10856.0
17	10627.0
18	10398.0
19	10834.0
20	11270.0
21	11799.5
22	12329.0
23	13921.0
24	15513.0
25	18302.0
26	21091.0
27	25302.5
28	29514.0
29	36075.5
30	42637.0
31	46625.0
32	50613.0
33	58464.5
34	66316.0
35	74233.0
36	82150.0
37	88734.0
38	95318.0
39	102395.0
40	109472.0
41	122436.0
42	135400.0
43	145749.5
44	156099.0
45	159426.0
46	162753.0
47	161337.0
48	159921.0
49	151890.5
50	143860.0
51	131938.5
52	120017.0
53	109471.5
54	98926.0
55	91091.5
56	83257.0
57	79550.0
58	75843.0
59	72023.5
60	68204.0
61	61991.5
62	55779.0
63	47913.0
64	40047.0
65	33741.0
66	27435.0
67	22489.0
68	17543.0
69	14932.5
70	12322.0
71	10370.5
72	8419.0
73	7213.0
74	6007.0
75	4837.0
76	3667.0
77	2770.5
78	1874.0
79	1399.0
80	924.0
81	721.0
82	518.0
83	398.0
84	278.0
85	215.5
86	153.0
87	103.5
88	54.0
89	40.0
90	26.0
91	17.5
92	9.0
93	8.0
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2013424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.920713239142327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9821332788169	18.414675347000145
2	6.049963084725149	2.894388641142145
3	2.5529306938638934	1.8320376914196852
4	1.6291472926287964	1.5588146084519412
5	1.1515757285122539	1.3773256387449022
6	0.9281336009907641	1.3320970630147568
7	0.7761021215022885	1.2995441404922368
8	0.6789175434407211	1.2992153495734757
9	0.5705901594676799	1.2284031223532577
>10	7.120348562659884	39.03982033916888
>50	1.317001113621776	21.44510428611854
>100	0.24126373453310831	7.466028779408996
>500	0.0012620568245299473	0.19290962310902315
>1k	4.206856081766491E-4	0.14601557805487356
>5k	2.1034280408832454E-4	0.473619791947114
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9413	0.467512059059592	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04767997202774974	0.0
2	0.0	0.0	0.0	0.1677739015726444	0.0
3	0.0	0.0	0.0	0.24684318851866274	0.0
4	0.0	0.0	0.0	0.37816177814509017	0.0
5	0.0	0.0	0.0	0.6139789731323357	0.0
6	0.0	0.0	0.0	1.0409133893308116	0.0
7	0.0	0.0	0.0	1.2579565953321308	0.0
8	0.0	0.0	0.0	1.8121369368796636	0.0
9	0.0	0.0	0.0	2.060768124349367	0.0
10	0.0	0.0	0.0	2.400289258496968	0.0
11	0.0	0.0	0.0	2.650956778105357	0.0
12	0.0	0.0	0.0	2.8799199771136132	0.0
13	0.0	0.0	0.0	3.0045832373111674	0.0
14	0.0	0.0	0.0	3.071583531337662	0.0
15	0.0	0.0	0.0	3.128004831570499	0.0
16	0.0	0.0	0.0	3.223563442176114	0.0
17	0.0	0.0	0.0	3.3253303824728424	0.0
18	0.0	0.0	0.0	3.4788996257122196	0.0
19	0.0	0.0	0.0	3.5474395855021097	0.0
20	0.0	0.0	0.0	3.625018873322261	0.0
21	0.0	0.0	0.0	3.7236071488171394	0.0
22	0.0	0.0	0.0	3.840174747097482	0.0
23	0.0	0.0	0.0	3.9647386740199777	0.0
24	0.0	0.0	0.0	4.067002280691995	0.0
25	0.0	0.0	0.0	4.156104228418853	0.0
26	0.0	0.0	0.0	4.240636845493051	0.0
27	0.0	0.0	0.0	4.335748456360905	0.0
28	0.0	0.0	0.0	4.431207733691463	0.0
29	0.0	0.0	0.0	4.530789341936919	0.0
30	0.0	0.0	0.0	4.679739587886108	0.0
31	0.0	0.0	0.0	4.793376854552245	0.0
32	0.0	0.0	0.0	4.8996137922265754	0.0
33	0.0	0.0	0.0	5.0040130643123355	0.0
34	0.0	0.0	0.0	5.114918665914383	0.0
35	0.0	0.0	0.0	5.240028925849697	0.0
36	0.0	0.0	0.0	5.355255524916759	0.0
37	0.0	0.0	0.0	5.474058121885902	0.0
38	0.0	0.0	0.0	5.6107407083654515	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATC	25	4.4440374E-5	44.0	39
TCAATCG	20	7.8578945E-4	44.0	37
AATCGCG	85	0.0	44.0	1
CGTTTTT	8475	0.0	41.430088	1
CGACAAT	240	0.0	38.5	20
GCGATAA	235	0.0	38.38298	9
TACGGGA	850	0.0	38.04706	4
AACGGGA	950	0.0	37.515793	4
AGGGCGA	1795	0.0	37.259052	6
ACGGGAT	1110	0.0	37.06306	5
CGACGGT	125	0.0	36.96	28
CGGGATT	1105	0.0	36.83258	6
CGTTAGG	365	0.0	36.767124	2
AGGGATT	4590	0.0	36.522877	6
GGGCGAT	4050	0.0	36.44938	7
TAGGGAT	3655	0.0	35.874146	5
TAAGGGA	2835	0.0	35.774254	4
ATAGGGA	3135	0.0	35.649124	4
GTAGGGT	830	0.0	35.253014	4
AGGGATC	2585	0.0	35.234043	6
>>END_MODULE