FastQCFastQC Report
Thu 26 May 2016
SRR1546610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546610_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2401753
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159880.6656804425767345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT156350.042.6351131
TATTGCG2050.039.7073171
GGTTACG950.039.368421
CCGGATA452.3528628E-839.1111120
ACGGGAC10050.038.965175
AGTACGG3350.038.746272
TACGACG800.038.51
GTTACGG2350.038.3829772
TACGGGA12750.038.305884
CGCACGA700.037.71428739
CGCGTAA357.293522E-637.71428731
TAGGGCG9350.037.647065
AGGGCGA27200.037.0441176
TTACGCG301.3015175E-436.6666681
AAGGGAT63950.036.465995
GCGCGAC1750.036.4571469
TAGTACG1750.036.4571461
TAGGGAC28850.036.3743485
TAGGGAT58450.036.2463655
GAGGGAT57200.036.1923075
Produced by FastQC (version 0.11.5)