##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546610_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2401753
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.984253168414902	31.0	31.0	33.0	30.0	34.0
2	31.35925509409169	31.0	31.0	34.0	30.0	34.0
3	31.479542234359652	31.0	31.0	34.0	30.0	34.0
4	35.27345609644289	37.0	35.0	37.0	33.0	37.0
5	35.07545655194352	37.0	35.0	37.0	32.0	37.0
6	35.15658437816045	37.0	35.0	37.0	32.0	37.0
7	35.71803553487807	37.0	35.0	37.0	35.0	37.0
8	35.76557997429378	37.0	35.0	37.0	35.0	37.0
9	37.52298508630987	39.0	37.0	39.0	35.0	39.0
10	36.766915248986884	39.0	37.0	39.0	32.0	39.0
11	36.36145515379808	38.0	35.0	39.0	32.0	39.0
12	36.05136956214898	38.0	35.0	39.0	31.0	39.0
13	35.97249675549484	38.0	35.0	39.0	31.0	39.0
14	36.95007313408165	39.0	35.0	41.0	31.0	41.0
15	37.09030237497361	39.0	35.0	41.0	31.0	41.0
16	37.096899223192395	39.0	35.0	41.0	31.0	41.0
17	36.99231228190409	39.0	35.0	41.0	31.0	41.0
18	36.88014754223269	38.0	35.0	40.0	31.0	41.0
19	36.81610515319436	38.0	35.0	40.0	31.0	41.0
20	36.64906382962778	38.0	35.0	40.0	31.0	41.0
21	36.561835042987354	38.0	35.0	40.0	31.0	41.0
22	36.490744468727634	38.0	35.0	40.0	30.0	41.0
23	36.283871821956716	38.0	35.0	40.0	30.0	41.0
24	36.23154629139633	38.0	35.0	40.0	30.0	41.0
25	36.087579780268825	38.0	34.0	40.0	30.0	41.0
26	35.977000132819654	38.0	34.0	40.0	30.0	41.0
27	35.788610652302715	38.0	34.0	40.0	29.0	41.0
28	35.744980437205655	38.0	34.0	40.0	29.0	41.0
29	35.62661647554932	38.0	34.0	40.0	29.0	41.0
30	35.56285804576907	38.0	34.0	40.0	28.0	41.0
31	35.47540650516518	38.0	34.0	40.0	27.0	41.0
32	35.21925027261338	38.0	34.0	40.0	26.0	41.0
33	34.924607567888955	38.0	34.0	40.0	25.0	41.0
34	34.7584451856623	38.0	34.0	40.0	24.0	41.0
35	34.512294561514025	38.0	33.0	40.0	23.0	41.0
36	34.31558823909037	38.0	33.0	40.0	23.0	41.0
37	34.38939849351703	38.0	33.0	40.0	23.0	41.0
38	34.31962903762377	38.0	33.0	40.0	23.0	41.0
39	34.23178278532389	38.0	33.0	40.0	22.0	41.0
40	34.23246353809072	38.0	33.0	40.0	22.0	41.0
41	34.25452242591141	38.0	33.0	40.0	22.0	41.0
42	34.00375350837493	37.0	33.0	40.0	21.0	41.0
43	34.0812210914278	37.0	33.0	40.0	21.0	41.0
44	33.89275916382742	37.0	33.0	40.0	20.0	41.0
45	33.65483336546264	37.0	33.0	40.0	19.0	41.0
46	33.75714280361053	37.0	33.0	40.0	20.0	41.0
47	33.64373605445689	37.0	32.0	40.0	20.0	41.0
48	33.69741059967449	37.0	33.0	40.0	20.0	41.0
49	33.61422094611727	37.0	32.0	40.0	20.0	41.0
50	33.152907480494456	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	20.0
15	56.0
16	202.0
17	616.0
18	1478.0
19	2863.0
20	5264.0
21	8302.0
22	12439.0
23	17828.0
24	25377.0
25	35941.0
26	48155.0
27	56132.0
28	59755.0
29	64714.0
30	74086.0
31	87420.0
32	106422.0
33	130571.0
34	179346.0
35	225170.0
36	203110.0
37	248634.0
38	340343.0
39	467504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.783556843688753	23.95641849932112	29.03949740044043	16.220527256549694
2	32.29536925737159	25.37879623758147	29.35243549190945	12.973399013137488
3	26.520379073118676	25.50253918700216	35.001559277744214	12.97552246213495
4	23.308516737566272	28.489898836391585	34.24140617290787	13.960178253134274
5	21.077979292625013	32.52166230249322	33.34847505134791	13.051883353533855
6	20.262866331383787	41.387103503149575	28.92279097808975	9.427239187376887
7	87.85024938034843	2.846920561773005	8.332913501096908	0.9699165567816507
8	88.67435577263774	2.1359815101719453	8.050161694395719	1.1395010227946005
9	82.7294063960782	5.52121721092885	9.672768182240223	2.0766082107527293
10	34.7183078359848	41.461923853118954	14.415220882413804	9.404547428482445
11	24.694088026537283	25.486176138845252	35.128300037514265	14.6914357971032
12	25.879888564727516	24.00867199916061	33.725616247799	16.38582318831287
13	21.214671117304736	28.890189790540493	34.12361720793104	15.771521884223732
14	18.565772583608723	31.216199167857813	32.896638413691996	17.32138983484147
15	16.995253050584303	26.382313252028833	39.6059045205731	17.01652917681377
16	19.70194270601515	25.117736919658267	38.50512521479103	16.675195159535555
17	20.07850099489831	24.104143931536672	33.6741538368017	22.143201236763314
18	20.83690537703086	25.012688648666202	35.84073799429001	18.309667980012932
19	22.965975268897342	26.365700386342812	32.20347804291282	18.464846301847025
20	24.876496459044706	26.38789251017902	31.40381213222176	17.331798898554513
21	22.502064117334296	27.22340723629782	32.88402262847179	17.390506017896097
22	22.30333427292482	25.37071880414014	32.10967156073085	20.21627536220419
23	19.39574968783218	28.641662985327798	32.16313251196106	19.799454814878967
24	18.617651357154543	26.612686650125973	37.53025394368197	17.239408049037515
25	18.917016029541756	27.84437034116331	34.83992733640803	18.398686292886904
26	18.780532386136294	30.48681525535723	31.813346334947852	18.919306023558626
27	18.441987997933175	30.088772658970342	32.9148334570624	18.554405886034075
28	17.60089401366419	28.591616206995475	35.731317916538465	18.07617186280188
29	18.284602954591918	26.033901071425745	35.84048817676089	19.841007797221447
30	19.02168957424015	28.813121082809097	33.95328328933075	18.21190605362
31	21.90595785661556	28.242496210059905	31.39261197966652	18.458933953658015
32	22.639255577072248	27.668831890706496	31.349435183384806	18.342477348836454
33	22.205280892747922	28.75662068497468	30.30973626347089	18.728362158806505
34	19.72330210475432	28.816243801923015	31.024047851714975	20.436406241607692
35	19.37838736955882	28.75828613516877	31.911816077673265	19.951510417599145
36	22.671024039524465	28.8321904875314	29.589553963292648	18.907231509651492
37	19.80732406704603	30.633541417456335	30.33334401997208	19.225790495525562
38	19.73874915530448	30.68458746590511	29.855567995543254	19.721095383247153
39	20.029453486682435	31.02279876406941	29.365051277129663	19.582696472118492
40	21.887471359461195	28.8340224827449	29.66524867461392	19.613257483179993
41	19.16212866185657	28.07936536354904	30.38272461822677	22.375781356367618
42	19.636115787093843	28.827027591929728	29.344691148506946	22.192165472469483
43	20.12505032782305	28.210061567530047	29.941755043087277	21.72313306155962
44	19.66059790494693	29.95720209363744	30.534488767162987	19.84771123425265
45	18.938167247006664	31.87126236544724	28.860628049595444	20.329942337950655
46	20.588545116837576	30.62163344856861	29.20533460351668	19.584486831077133
47	19.70331670242527	28.92445642828384	30.14489833051109	21.2273285387798
48	20.295800608971863	28.30228587202764	31.191383959965908	20.210529559034587
49	20.20828120127257	27.632275258946276	31.83720390897815	20.322239630803
50	19.256018416548244	30.784556113805206	30.15222631136507	19.80719915828147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1775.0
1	2201.5
2	2628.0
3	16676.5
4	30725.0
5	23696.0
6	16667.0
7	17160.0
8	17653.0
9	18259.5
10	18866.0
11	19055.5
12	19245.0
13	19098.0
14	18951.0
15	18581.0
16	18211.0
17	17744.5
18	17278.0
19	17205.5
20	17133.0
21	18906.5
22	20680.0
23	22144.5
24	23609.0
25	27023.0
26	30437.0
27	38088.0
28	45739.0
29	53852.0
30	61965.0
31	69685.5
32	77406.0
33	87725.5
34	98045.0
35	107864.5
36	117684.0
37	124507.5
38	131331.0
39	140888.0
40	150445.0
41	166953.5
42	183462.0
43	188873.5
44	194285.0
45	192985.0
46	191685.0
47	184107.0
48	176529.0
49	167393.5
50	158258.0
51	143104.0
52	127950.0
53	115505.5
54	103061.0
55	90605.0
56	78149.0
57	71800.0
58	65451.0
59	59681.0
60	53911.0
61	49084.0
62	44257.0
63	37683.0
64	31109.0
65	26037.5
66	20966.0
67	17025.0
68	13084.0
69	10744.5
70	8405.0
71	7107.5
72	5810.0
73	4769.0
74	3728.0
75	3118.0
76	2508.0
77	1953.0
78	1398.0
79	936.0
80	474.0
81	430.5
82	387.0
83	319.0
84	251.0
85	181.5
86	112.0
87	77.0
88	42.0
89	23.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2401753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.78604177144557
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.36448579074026	19.175593764356954
2	6.3005602396266776	3.123318985657919
3	2.548405998663665	1.894946926004402
4	1.626789686984324	1.6128670853980127
5	1.1743303115598758	1.4553500077898887
6	0.9331809459577249	1.3877917144095158
7	0.7461917128387471	1.2946597274749394
8	0.6450806052553907	1.2791195862247604
9	0.5712733038003139	1.2743643573815668
>10	6.6086489510510535	37.631176782532705
>50	1.2234082606857255	20.758130737888177
>100	0.25679504842633777	8.295301797311653
>500	5.094866460383901E-4	0.09045970005905656
>1k	1.698288820127967E-4	0.053922231631294296
>5k	0.0	0.0
>10k+	1.698288820127967E-4	0.6729965958791049
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15988	0.6656804425767345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027646473221850872	0.0
2	0.0	0.0	0.0	0.0907670355777634	0.0
3	0.0	0.0	0.0	0.1408970864197942	0.0
4	0.0	0.0	0.0	0.21105417584572603	0.0
5	0.0	0.0	0.0	0.34287455870774386	0.0
6	0.0	0.0	0.0	0.6008944300267346	0.0
7	0.0	0.0	0.0	0.7375862547064581	0.0
8	0.0	0.0	0.0	1.0755893715964964	0.0
9	0.0	0.0	0.0	1.2464229252550116	0.0
10	0.0	0.0	0.0	1.4945333679191823	0.0
11	0.0	0.0	0.0	1.6811470621666758	0.0
12	0.0	0.0	0.0	1.8441529999129802	0.0
13	0.0	0.0	0.0	1.9408740199346062	0.0
14	0.0	0.0	0.0	1.995750603829786	0.0
15	0.0	0.0	0.0	2.0437155694195033	0.0
16	0.0	0.0	0.0	2.128695165572813	0.0
17	0.0	0.0	0.0	2.2282474509243873	0.0
18	0.0	0.0	0.0	2.365647091936598	0.0
19	4.163625485218505E-5	0.0	0.0	2.4374696315566173	0.0
20	4.163625485218505E-5	0.0	0.0	2.515662518169021	0.0
21	4.163625485218505E-5	0.0	0.0	2.6092608190767326	0.0
22	4.163625485218505E-5	0.0	0.0	2.716432539066257	0.0
23	4.163625485218505E-5	0.0	0.0	2.8363865892954023	0.0
24	4.163625485218505E-5	0.0	0.0	2.9318585216714625	0.0
25	4.163625485218505E-5	0.0	0.0	3.018586840528564	0.0
26	4.163625485218505E-5	0.0	0.0	3.101109897645595	0.0
27	4.163625485218505E-5	0.0	0.0	3.194500017279046	0.0
28	4.163625485218505E-5	0.0	0.0	3.28947231459688	0.0
29	4.163625485218505E-5	0.0	0.0	3.397060397134926	0.0
30	4.163625485218505E-5	0.0	0.0	3.5370831222028243	4.163625485218505E-5
31	4.163625485218505E-5	0.0	0.0	3.654913723434508	4.163625485218505E-5
32	4.163625485218505E-5	0.0	0.0	3.7707041481784347	4.163625485218505E-5
33	4.163625485218505E-5	0.0	0.0	3.8883265681358576	4.163625485218505E-5
34	4.163625485218505E-5	0.0	0.0	4.019272589645979	4.163625485218505E-5
35	4.163625485218505E-5	0.0	0.0	4.163583848963653	4.163625485218505E-5
36	4.163625485218505E-5	0.0	0.0	4.294488234218923	4.163625485218505E-5
37	4.163625485218505E-5	0.0	0.0	4.431304967663203	4.163625485218505E-5
38	4.163625485218505E-5	0.0	0.0	4.576282407058511	4.163625485218505E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15635	0.0	42.635113	1
TATTGCG	205	0.0	39.707317	1
GGTTACG	95	0.0	39.36842	1
CCGGATA	45	2.3528628E-8	39.11111	20
ACGGGAC	1005	0.0	38.96517	5
AGTACGG	335	0.0	38.74627	2
TACGACG	80	0.0	38.5	1
GTTACGG	235	0.0	38.382977	2
TACGGGA	1275	0.0	38.30588	4
CGCACGA	70	0.0	37.714287	39
CGCGTAA	35	7.293522E-6	37.714287	31
TAGGGCG	935	0.0	37.64706	5
AGGGCGA	2720	0.0	37.044117	6
TTACGCG	30	1.3015175E-4	36.666668	1
AAGGGAT	6395	0.0	36.46599	5
GCGCGAC	175	0.0	36.457146	9
TAGTACG	175	0.0	36.457146	1
TAGGGAC	2885	0.0	36.374348	5
TAGGGAT	5845	0.0	36.246365	5
GAGGGAT	5720	0.0	36.192307	5
>>END_MODULE