FastQCFastQC Report
Thu 26 May 2016
SRR1546609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546609_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences957652
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33120.3458458813848872No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23210.24236361434007342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12530.13084084824132358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG600.044.0000041
ACGCCGA207.855822E-444.00000412
ACGTTAG207.855822E-444.0000041
CGTTCGA302.527273E-644.00000414
TATGACG408.309144E-944.0000041
TAACCGA207.855822E-444.00000437
TACGAGT207.855822E-444.00000413
CGTAGAT302.527273E-644.00000426
TTGCGAC207.855822E-444.00000422
CGGTAAG207.855822E-444.0000042
TCGACAC302.527273E-644.00000434
GTTGCCG207.855822E-444.00000422
TATCCGA302.527273E-644.00000432
GAATCTA551.8189894E-1244.019
TCGATAC254.4422814E-544.031
ATACTCG351.445751E-744.044
GCCGATC351.445751E-744.09
CTCGCGG254.4422814E-544.01
CCGACAT254.4422814E-544.036
CGGTCTA502.7284841E-1144.031
Produced by FastQC (version 0.11.5)