##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546609_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	957652
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.004522519662675	31.0	31.0	33.0	30.0	34.0
2	31.373291132895876	31.0	31.0	34.0	30.0	34.0
3	31.518385593096447	31.0	31.0	34.0	30.0	34.0
4	35.290393587649795	37.0	35.0	37.0	33.0	37.0
5	35.08008128213589	37.0	35.0	37.0	32.0	37.0
6	35.152036439124025	37.0	35.0	37.0	32.0	37.0
7	35.654169781924956	37.0	35.0	37.0	35.0	37.0
8	35.703609453120755	37.0	35.0	37.0	35.0	37.0
9	37.47707622393103	39.0	37.0	39.0	35.0	39.0
10	36.77456215827879	39.0	37.0	39.0	32.0	39.0
11	36.31990013073643	38.0	35.0	39.0	32.0	39.0
12	35.29414442824742	37.0	35.0	39.0	30.0	39.0
13	34.855356643122974	37.0	33.0	39.0	28.0	39.0
14	35.67768354266477	37.0	34.0	40.0	27.0	41.0
15	36.08989382364366	37.0	34.0	40.0	30.0	41.0
16	36.26648719994319	37.0	35.0	40.0	31.0	41.0
17	36.21101506601563	37.0	34.0	40.0	31.0	41.0
18	36.188855659467116	37.0	35.0	40.0	31.0	41.0
19	36.08921299177572	37.0	35.0	40.0	31.0	41.0
20	35.90310467685548	36.0	34.0	40.0	30.0	41.0
21	35.77554790257839	36.0	34.0	40.0	30.0	41.0
22	35.67028001821121	36.0	34.0	40.0	30.0	41.0
23	35.5425968932347	36.0	34.0	40.0	30.0	41.0
24	35.477550300109016	35.0	34.0	40.0	30.0	41.0
25	35.361480997272494	35.0	34.0	40.0	29.0	41.0
26	35.215304724471935	35.0	34.0	40.0	29.0	41.0
27	35.038302013675114	35.0	34.0	40.0	29.0	41.0
28	35.05314143342258	36.0	34.0	40.0	29.0	41.0
29	35.02427499759829	36.0	34.0	40.0	29.0	41.0
30	34.96492567237368	36.0	34.0	40.0	29.0	41.0
31	34.791227919954224	35.0	34.0	40.0	27.0	41.0
32	34.53278435172693	35.0	33.0	40.0	26.0	41.0
33	34.30350168954902	35.0	33.0	40.0	25.0	41.0
34	34.17669779836517	35.0	33.0	40.0	24.0	41.0
35	33.95073784631578	35.0	33.0	40.0	23.0	41.0
36	33.61470450643866	35.0	33.0	39.0	23.0	41.0
37	33.61442152264079	35.0	33.0	40.0	23.0	41.0
38	33.58329330487484	35.0	33.0	40.0	23.0	41.0
39	33.523680836044825	35.0	33.0	40.0	23.0	41.0
40	33.400804258749524	35.0	32.0	40.0	22.0	41.0
41	33.44768872199922	35.0	33.0	40.0	22.0	41.0
42	33.19913183494631	35.0	32.0	39.0	21.0	41.0
43	33.24806819178574	35.0	32.0	39.0	21.0	41.0
44	33.0784397672641	35.0	32.0	39.0	20.0	41.0
45	32.939105228203985	35.0	32.0	39.0	20.0	41.0
46	32.98599491255696	35.0	32.0	39.0	20.0	41.0
47	32.870649254635296	35.0	32.0	39.0	20.0	41.0
48	32.92364032028336	35.0	32.0	39.0	20.0	41.0
49	32.86001282302966	35.0	32.0	39.0	20.0	41.0
50	32.313112696470114	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	9.0
15	31.0
16	88.0
17	276.0
18	674.0
19	1389.0
20	2288.0
21	3730.0
22	5683.0
23	7995.0
24	11417.0
25	15343.0
26	19115.0
27	22354.0
28	24899.0
29	28435.0
30	33692.0
31	40535.0
32	49578.0
33	63055.0
34	103052.0
35	143774.0
36	67188.0
37	76516.0
38	100700.0
39	135830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.10028277495374	25.404217816075153	29.32892115298668	13.16657825598443
2	28.96647216316574	27.38280711573724	30.630020090805427	13.020700630291588
3	26.66991767364345	27.10170291504638	32.21180554105249	14.016573870257673
4	23.91213092020901	30.21337604891965	31.76038895131008	14.11410407956126
5	22.349559130038887	34.85065556172806	30.08963590114154	12.710149407091512
6	21.135026084631996	41.62200882992987	27.043226558290485	10.199738527147648
7	86.73046158729893	5.356121012643424	6.556661501255154	1.3567558988024877
8	87.48501543358131	4.373927063275595	5.696119258352721	2.444938244790383
9	82.91091127048239	6.339568026798879	8.128422433201205	2.6210982695175282
10	50.396281739086845	27.26731631114434	12.403044112057406	9.933357837711403
11	45.39968589842657	21.045327530251072	21.8517791431543	11.70320742816806
12	42.161348798937404	22.542322263202085	22.381303437992088	12.91502549986843
13	21.068613650887798	43.398019322259024	23.443589111702373	12.089777915150806
14	14.682786649012375	45.36073646794451	26.15052231917231	13.8059545638708
15	12.924423485775627	24.941732487375372	48.483791606972055	13.650052419876948
16	13.66477593113156	19.98304185654079	48.69211362791494	17.66006858441271
17	14.655323645750231	21.217623938549703	29.699619485992823	34.427432929707244
18	18.78365001065105	24.200753509625624	37.22020107512959	19.795395404593734
19	27.175842581647615	25.23870884204283	26.736643373584556	20.848805202725
20	28.97284190916951	23.972173607949443	27.301357904541522	19.75362657833952
21	19.944301270190007	31.601980677740976	29.408699611132228	19.045018440936794
22	21.42364867404861	27.310651468383085	24.65373643035257	26.61196342721573
23	17.505106239009578	32.92145789911158	24.356968919816385	25.21646694206246
24	16.847873757899528	25.246540496965498	41.818740001587216	16.086845743547762
25	14.67401519549899	27.180750418732487	37.87273456328604	20.272499822482487
26	15.169393474873964	36.4391240241758	28.242931670377132	20.148550830573107
27	15.761362164961804	38.895131007923545	27.189626294311502	18.15388053280315
28	14.377978639422254	32.72430903919169	36.977837460789516	15.919874860596542
29	14.248704122165462	25.88539469452369	37.15692130335446	22.70897987995639
30	17.02831508731773	35.2419250416644	30.563607657061233	17.166152213956636
31	27.40275173027363	29.841737917322785	25.133869088144756	17.621641264258834
32	29.991688003575412	26.45345073158099	27.69429813752804	15.86056312731556
33	29.62297368981634	28.485295284717203	23.933224177467388	17.958506847999065
34	18.961480788428364	27.95232506171344	27.867743188548662	25.218450961309536
35	20.051333887466427	26.210878273109646	31.40712910326507	22.330658736158856
36	30.91571886238425	24.37503393717133	26.976918546611923	17.7323286538325
37	19.926236252835057	34.1751492191318	29.004795061253986	16.893819466779163
38	19.517632710003216	34.278422642045335	25.69284040549176	20.511104242459684
39	19.889479685731352	34.17859514729776	27.825347829900632	18.106577337070252
40	24.8592390555233	25.590611203234577	26.568001737583174	22.98214800365895
41	17.817850325588	23.92340850329765	28.90736927401603	29.35137189709832
42	19.799885553416065	25.223672064591312	27.030382644217315	27.946059737775307
43	20.127039885052188	25.509788524432675	28.660202244656723	25.702969345858413
44	18.095926286375427	30.148947634422523	29.86606825861586	21.88905782058618
45	16.09874985903021	38.974387355740916	23.654103996023608	21.272758789205266
46	21.113097450848535	34.49854435640504	25.593743865203646	18.79461432754278
47	20.525514487517384	28.828217348264296	26.805039826575833	23.841228337642484
48	21.137845480404156	26.090166365234968	32.56819805106657	20.20379010329431
49	21.85762677882989	24.657077936452907	33.320245767773685	20.165049516943526
50	20.235638833313143	33.885377986993184	26.97430799497103	18.904675184722635
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1633.0
1	1733.0
2	1833.0
3	4107.5
4	6382.0
5	5076.0
6	3770.0
7	4250.5
8	4731.0
9	5489.0
10	6247.0
11	6553.5
12	6860.0
13	7158.5
14	7457.0
15	7214.0
16	6971.0
17	6952.5
18	6934.0
19	6760.5
20	6587.0
21	6305.0
22	6023.0
23	6396.0
24	6769.0
25	7602.5
26	8436.0
27	9578.5
28	10721.0
29	12954.0
30	15187.0
31	16492.5
32	17798.0
33	20764.0
34	23730.0
35	26251.0
36	28772.0
37	30571.5
38	32371.0
39	42706.0
40	53041.0
41	72230.0
42	91419.0
43	100354.5
44	109290.0
45	106193.5
46	103097.0
47	99158.0
48	95219.0
49	86656.0
50	78093.0
51	67019.0
52	55945.0
53	48035.0
54	40125.0
55	33286.5
56	26448.0
57	25075.0
58	23702.0
59	21768.0
60	19834.0
61	17735.5
62	15637.0
63	13718.5
64	11800.0
65	10304.5
66	8809.0
67	7170.5
68	5532.0
69	4689.5
70	3847.0
71	3071.5
72	2296.0
73	2119.0
74	1942.0
75	1497.5
76	1053.0
77	890.0
78	727.0
79	528.0
80	329.0
81	233.0
82	137.0
83	103.0
84	69.0
85	52.5
86	36.0
87	22.0
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	957652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.779767897838713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.83188912597642	20.277752191833677
2	6.272980093940335	3.10885981511208
3	2.2922330812379843	1.704030111624748
4	1.2887508732590935	1.277397900699892
5	0.8250342625949314	1.022207876743346
6	0.6438684439119898	0.9572946358129015
7	0.4866727012277947	0.8441745605047272
8	0.39268368585605995	0.7784488474224782
9	0.3279637765421551	0.7314179635451921
>10	3.7798284463926466	21.808409864653115
>50	1.1788739384833458	20.515774421312035
>100	0.6758254627243397	25.9371295335708
>500	0.0021225674080538304	0.31274014301771536
>1k	0.0012735404448322985	0.7243621341473219
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3312	0.3458458813848872	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2321	0.24236361434007342	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1253	0.13084084824132358	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07831654922665018	0.0
2	0.0	0.0	0.0	0.2827749537410249	0.0
3	0.0	0.0	0.0	0.3928358109208773	0.0
4	0.0	0.0	0.0	0.6415691712647182	0.0
5	0.0	0.0	0.0	0.962667023093984	0.0
6	0.0	0.0	0.0	1.5656000300735549	0.0
7	0.0	0.0	0.0	1.8361576021352224	0.0
8	0.0	0.0	0.0	2.644279968088617	0.0
9	0.0	0.0	0.0	3.028448747561745	0.0
10	0.0	0.0	0.0	3.6376470784794477	0.0
11	0.0	0.0	0.0	4.089168090287495	0.0
12	0.0	0.0	0.0	4.469577675397744	0.0
13	0.0	0.0	0.0	4.6677707559739865	0.0
14	0.0	0.0	0.0	4.758095842748723	0.0
15	0.0	0.0	0.0	4.837456612631728	0.0
16	0.0	0.0	0.0	5.007560157552013	0.0
17	0.0	0.0	0.0	5.177141592144119	0.0
18	0.0	0.0	0.0	5.449683183452862	0.0
19	0.0	0.0	0.0	5.559326352370172	0.0
20	0.0	0.0	0.0	5.6793073057854	0.0
21	0.0	0.0	0.0	5.839386332404673	0.0
22	0.0	0.0	0.0	6.010011987653135	0.0
23	0.0	0.0	0.0	6.195883264484385	0.0
24	0.0	0.0	0.0	6.3395680267988785	0.0
25	0.0	0.0	0.0	6.454119032801059	0.0
26	0.0	0.0	0.0	6.57075848011595	0.0
27	0.0	0.0	0.0	6.690530589399907	0.0
28	0.0	0.0	0.0	6.805186017467723	0.0
29	0.0	0.0	0.0	6.936444553971588	0.0
30	0.0	0.0	0.0	7.128894420937877	0.0
31	0.0	0.0	0.0	7.273832248039998	0.0
32	0.0	0.0	0.0	7.407388069987845	0.0
33	0.0	0.0	0.0	7.526429224812354	0.0
34	0.0	0.0	0.0	7.666354792763968	0.0
35	0.0	0.0	0.0	7.845229791197638	0.0
36	0.0	0.0	0.0	7.993613546465731	0.0
37	0.0	0.0	0.0	8.141057503143104	0.0
38	0.0	0.0	0.0	8.291320855592637	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	60	0.0	44.000004	1
ACGCCGA	20	7.855822E-4	44.000004	12
ACGTTAG	20	7.855822E-4	44.000004	1
CGTTCGA	30	2.527273E-6	44.000004	14
TATGACG	40	8.309144E-9	44.000004	1
TAACCGA	20	7.855822E-4	44.000004	37
TACGAGT	20	7.855822E-4	44.000004	13
CGTAGAT	30	2.527273E-6	44.000004	26
TTGCGAC	20	7.855822E-4	44.000004	22
CGGTAAG	20	7.855822E-4	44.000004	2
TCGACAC	30	2.527273E-6	44.000004	34
GTTGCCG	20	7.855822E-4	44.000004	22
TATCCGA	30	2.527273E-6	44.000004	32
GAATCTA	55	1.8189894E-12	44.0	19
TCGATAC	25	4.4422814E-5	44.0	31
ATACTCG	35	1.445751E-7	44.0	44
GCCGATC	35	1.445751E-7	44.0	9
CTCGCGG	25	4.4422814E-5	44.0	1
CCGACAT	25	4.4422814E-5	44.0	36
CGGTCTA	50	2.7284841E-11	44.0	31
>>END_MODULE