FastQCFastQC Report
Thu 26 May 2016
SRR1546608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546608_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2387519
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80980.3391805468354388No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29660.12422937786044845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT1600.044.00000428
CGTTCGA408.3182385E-944.00000414
CTAGTCG408.3182385E-944.0000041
CTACGCG408.3182385E-944.0000041
CGCGCGA254.4442855E-544.016
TACGTCG254.4442855E-544.01
CGGTCTA1600.042.62500431
CGTTTTT81000.041.9901241
TCACGAC1750.041.48571425
GTTAGCG1500.041.0666661
GCTAGCG2000.040.71
CGTTTGG1400.039.2857132
GGCGATA9750.039.261548
CACGACG1800.039.11110726
GACGGTC1800.039.11110729
GCGTAAG3000.038.8666651
AAGCGAC404.1270323E-738.50000415
CGCACGA404.1270323E-738.50000439
TCGACAG800.038.5000041
CGTAAGG4900.038.1632652
Produced by FastQC (version 0.11.5)