##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546608_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2387519
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.937436728252216	31.0	31.0	33.0	30.0	34.0
2	31.307814094882595	31.0	31.0	34.0	30.0	34.0
3	31.463468981817527	31.0	31.0	34.0	30.0	34.0
4	35.23061093964069	37.0	35.0	37.0	33.0	37.0
5	35.00804977887087	35.0	35.0	37.0	32.0	37.0
6	35.088359506248956	37.0	35.0	37.0	32.0	37.0
7	35.68168462743124	37.0	35.0	37.0	35.0	37.0
8	35.72887461838	37.0	35.0	37.0	35.0	37.0
9	37.49409240303428	39.0	37.0	39.0	35.0	39.0
10	36.710447121049086	39.0	37.0	39.0	32.0	39.0
11	36.25917741387608	38.0	35.0	39.0	31.0	39.0
12	35.76528982596578	37.0	35.0	39.0	30.0	39.0
13	35.58100689460482	37.0	35.0	39.0	30.0	39.0
14	36.512789217593664	38.0	35.0	40.0	30.0	41.0
15	36.74135033061517	38.0	35.0	40.0	31.0	41.0
16	36.796363924224266	38.0	35.0	40.0	31.0	41.0
17	36.716829478634516	38.0	35.0	40.0	31.0	41.0
18	36.63803890147052	38.0	35.0	40.0	31.0	41.0
19	36.600741606663654	38.0	35.0	40.0	31.0	41.0
20	36.474245021715014	38.0	35.0	40.0	31.0	41.0
21	36.35033480361832	38.0	35.0	40.0	30.0	41.0
22	36.28900544875245	38.0	34.0	40.0	30.0	41.0
23	36.10877986730158	38.0	34.0	40.0	30.0	41.0
24	36.07353574987257	38.0	34.0	40.0	30.0	41.0
25	35.94188821115141	38.0	34.0	40.0	30.0	41.0
26	35.82182508285798	38.0	34.0	40.0	29.0	41.0
27	35.633281661842275	37.0	34.0	40.0	29.0	41.0
28	35.62777845956409	37.0	34.0	40.0	29.0	41.0
29	35.5548646942705	37.0	34.0	40.0	29.0	41.0
30	35.528022604218016	37.0	34.0	40.0	29.0	41.0
31	35.453587175641324	37.0	34.0	40.0	28.0	41.0
32	35.24231430200137	37.0	34.0	40.0	27.0	41.0
33	35.03498485247657	37.0	34.0	40.0	26.0	41.0
34	34.97645421879365	37.0	34.0	40.0	26.0	41.0
35	34.77637413566133	37.0	33.0	40.0	25.0	41.0
36	34.568245949037475	37.0	33.0	40.0	24.0	41.0
37	34.637171054973805	37.0	33.0	40.0	24.0	41.0
38	34.61486714870122	37.0	33.0	40.0	24.0	41.0
39	34.5433234248607	37.0	33.0	40.0	24.0	41.0
40	34.53317355799053	37.0	33.0	40.0	23.0	41.0
41	34.57373072214295	37.0	33.0	40.0	24.0	41.0
42	34.32460684082514	37.0	33.0	40.0	23.0	41.0
43	34.380538123466245	37.0	33.0	40.0	23.0	41.0
44	34.208331745213336	37.0	33.0	40.0	23.0	41.0
45	33.96716382152351	37.0	33.0	40.0	23.0	41.0
46	34.09117707544945	37.0	33.0	40.0	23.0	41.0
47	33.96208658444184	37.0	33.0	40.0	23.0	41.0
48	34.04028198309626	37.0	33.0	40.0	23.0	41.0
49	33.96478603939906	36.0	33.0	40.0	23.0	41.0
50	33.46116240331491	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	5.0
14	21.0
15	61.0
16	249.0
17	686.0
18	1470.0
19	2924.0
20	5012.0
21	7707.0
22	11751.0
23	16794.0
24	23328.0
25	31935.0
26	41120.0
27	48890.0
28	55555.0
29	63863.0
30	75958.0
31	91430.0
32	111279.0
33	137025.0
34	198230.0
35	260056.0
36	191434.0
37	231615.0
38	319830.0
39	459285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.22416240457144	24.57668399706976	29.495513962401976	13.703639635956824
2	29.56298986521154	26.2340948909726	30.39037595093484	13.81253929288102
3	27.552911620808047	26.667264218630304	32.30181623685508	13.478007923706576
4	23.3465786031441	28.957968502030766	33.1869191407482	14.50853375407693
5	21.782653876262344	34.33660632648368	30.492867281893883	13.387872515360085
6	20.7269135868657	41.96272364743485	27.324976261969013	9.98538650373044
7	89.76975680612385	3.5803694127669767	5.5638510101909135	1.0860227709182628
8	90.6197186284172	2.735894457803268	5.092566802609738	1.5518201111697958
9	85.33071359850958	5.665420882514443	6.928824440768848	2.0750410782071262
10	39.77610230536385	39.67093036746514	11.443092180627673	9.109875146543336
11	29.841019066235702	25.124910000716227	30.83954515126372	14.194525781784353
12	29.90464159656949	24.617772675316928	29.627827045564874	15.849758682548703
13	20.604945971110595	34.70577616345671	30.169686607729613	14.51959125770308
14	17.04656591214562	36.785508303808264	29.68382659991397	16.48409918413215
15	15.41859981009575	27.403719090821895	41.49269597435664	15.684985124725708
16	17.73267563525149	24.654337829353402	40.659152869568786	16.953833665826323
17	18.137908012459796	24.573040047011144	31.146767837240247	26.14228410328881
18	19.656639381718012	26.20238833701428	35.15176214304472	18.989210138222983
19	24.087515115062956	27.207029556623425	29.639135856091613	19.066319472222002
20	26.217550520016804	26.295665081618196	29.568560501508053	17.918223896856947
21	21.635974415282142	30.195738756424557	30.706520031882466	17.461766796410835
22	21.847072211781352	26.980266963320503	28.48593037374781	22.686730451150336
23	17.619503761017192	31.5691309681724	29.33744192192816	21.47392334888225
24	17.443840237501774	27.573686324590508	38.01779169087241	16.96468174703531
25	17.05448207951434	28.938366563784413	35.12797175645513	18.87917960024611
26	17.444677927170424	33.875793239760604	30.141414581412757	18.538114251656218
27	17.285810081511393	34.10000087957415	30.653913120691396	17.960275918223058
28	16.218551559170837	30.813074157734448	35.20382455595118	17.76454972714353
29	16.25566121149193	27.100098470420548	36.229282363826215	20.414957954261308
30	17.932422736740524	31.614575632696535	32.45758463074011	17.995416999822826
31	23.154538246606624	29.84235936970554	28.829760098244243	18.173342285443592
32	24.454465074414067	28.29204709993931	29.373546346646872	17.879941478999747
33	23.663392835826645	29.45417397725421	28.710766280812845	18.171666906106296
34	18.93044620796735	29.763197696018334	29.18087772285791	22.1254783731564
35	18.54464823107167	28.376863178889884	31.78693028202079	21.291558308017652
36	24.460580208995193	28.224864388513765	28.503396203339115	18.811159199151923
37	18.93325246835732	32.43777327007659	30.197958634046472	18.431015627519614
38	19.106821767701117	32.99349659625746	27.707716671574133	20.1919649644673
39	19.390631027438943	32.61762524193525	28.494977422169203	19.496766308456603
40	22.52581026580312	28.624316707008408	28.30959669849748	20.540276328690997
41	18.291163337338887	27.941557742577128	29.42983909238	24.33743982770399
42	19.563530175047823	28.325052072884027	27.843212975477893	24.26820477659026
43	19.455719514692866	28.236340736974242	29.04387357755059	23.264066170782304
44	18.712772547569255	30.55100294489803	30.29864893221792	20.437575575314792
45	17.359024158551197	34.63197570364885	27.43953032415658	20.56946981364337
46	20.309660362912297	32.94352840752262	27.87931740019661	18.867493829368478
47	19.30271549671437	29.71017193999294	29.072313141801175	21.914799421491516
48	20.53893602522116	27.665036382956533	31.933651627484434	19.862375964337875
49	20.4124867697388	27.194003482275953	32.148895987843446	20.244613760141807
50	19.02359729912097	32.05494909150461	29.56625685491927	19.355196754455147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2495.0
1	2698.0
2	2901.0
3	9515.5
4	16130.0
5	12950.0
6	9770.0
7	10449.5
8	11129.0
9	12033.5
10	12938.0
11	13414.0
12	13890.0
13	13946.5
14	14003.0
15	14065.5
16	14128.0
17	13806.5
18	13485.0
19	14055.5
20	14626.0
21	16078.5
22	17531.0
23	20194.0
24	22857.0
25	26962.5
26	31068.0
27	33755.5
28	36443.0
29	45362.5
30	54282.0
31	63779.0
32	73276.0
33	83426.5
34	93577.0
35	104392.0
36	115207.0
37	125022.5
38	134838.0
39	148535.5
40	162233.0
41	181765.5
42	201298.0
43	221857.5
44	242417.0
45	235553.5
46	228690.0
47	217142.5
48	205595.0
49	192407.5
50	179220.0
51	155125.0
52	131030.0
53	111661.5
54	92293.0
55	80231.5
56	68170.0
57	60119.5
58	52069.0
59	46326.0
60	40583.0
61	35318.5
62	30054.0
63	25059.5
64	20065.0
65	15500.0
66	10935.0
67	9050.0
68	7165.0
69	5645.0
70	4125.0
71	3668.0
72	3211.0
73	2321.0
74	1431.0
75	1234.0
76	1037.0
77	891.5
78	746.0
79	536.5
80	327.0
81	233.5
82	140.0
83	103.5
84	67.0
85	44.5
86	22.0
87	16.0
88	10.0
89	7.5
90	5.0
91	6.0
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2387519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.767836111285973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.25357075482006	17.469465755364162
2	6.703090713057221	2.9182356016182522
3	2.38630616904205	1.558341647971741
4	1.2842543303401115	1.1182175115221147
5	0.896865260431436	0.9761408001488656
6	0.6354317450696632	0.8299184451950927
7	0.5216989173211174	0.7949379572176989
8	0.40850955799983285	0.7113895286747387
9	0.37174337372016697	0.7282843951137415
>10	4.336361069910917	22.008914120730722
>50	1.3334380717216885	20.75256343069758
>100	0.8642470258522543	29.06452106896128
>500	0.003898270185678266	0.5193666963517235
>1k	3.898270185678266E-4	0.20611742593551774
>5k	1.949135092839133E-4	0.3435856144968758
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8098	0.3391805468354388	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2966	0.12422937786044845	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.036565154036470496	0.0
2	0.0	0.0	0.0	0.13449107630138232	0.0
3	0.0	0.0	0.0	0.19350631345761019	0.0
4	0.0	0.0	0.0	0.3195367241056511	0.0
5	0.0	0.0	0.0	0.5189906342106597	0.0
6	0.0	0.0	0.0	0.9000137799950493	0.0
7	0.0	0.0	0.0	1.0944834365716043	0.0
8	0.0	0.0	0.0	1.6404476781127186	0.0
9	0.0	0.0	0.0	1.908508372079971	0.0
10	0.0	0.0	0.0	2.310138683713093	0.0
11	0.0	0.0	0.0	2.611162466141631	0.0
12	0.0	0.0	0.0	2.8857152550409024	0.0
13	0.0	0.0	0.0	3.037420854032994	0.0
14	0.0	0.0	0.0	3.115786722534983	0.0
15	0.0	0.0	0.0	3.183346394311417	0.0
16	0.0	0.0	0.0	3.303722399696086	0.0
17	0.0	0.0	0.0	3.449564171007644	0.0
18	0.0	0.0	0.0	3.6486411207617615	0.0
19	0.0	0.0	0.0	3.744472818855054	0.0
20	0.0	0.0	0.0	3.8468384963637985	0.0
21	0.0	0.0	0.0	3.9668794258810087	0.0
22	0.0	0.0	0.0	4.10995682128603	0.0
23	0.0	0.0	0.0	4.270500046282354	0.0
24	0.0	0.0	0.0	4.397074955215016	0.0
25	0.0	0.0	0.0	4.504718077636241	0.0
26	0.0	0.0	0.0	4.611649163839115	0.0
27	0.0	0.0	0.0	4.720129975928987	0.0
28	0.0	0.0	0.0	4.831417048408829	0.0
29	0.0	0.0	0.0	4.959206607361031	0.0
30	0.0	0.0	0.0	5.121132020310624	0.0
31	0.0	0.0	0.0	5.268774824409775	0.0
32	0.0	0.0	0.0	5.409632342192879	0.0
33	0.0	0.0	0.0	5.550154784108525	0.0
34	0.0	0.0	0.0	5.689755767388657	0.0
35	0.0	0.0	0.0	5.854990054529409	0.0
36	0.0	0.0	0.0	6.009711336328633	0.0
37	0.0	0.0	0.0	6.170966597543307	0.0
38	0.0	0.0	0.0	6.331174746672173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	160	0.0	44.000004	28
CGTTCGA	40	8.3182385E-9	44.000004	14
CTAGTCG	40	8.3182385E-9	44.000004	1
CTACGCG	40	8.3182385E-9	44.000004	1
CGCGCGA	25	4.4442855E-5	44.0	16
TACGTCG	25	4.4442855E-5	44.0	1
CGGTCTA	160	0.0	42.625004	31
CGTTTTT	8100	0.0	41.990124	1
TCACGAC	175	0.0	41.485714	25
GTTAGCG	150	0.0	41.066666	1
GCTAGCG	200	0.0	40.7	1
CGTTTGG	140	0.0	39.285713	2
GGCGATA	975	0.0	39.26154	8
CACGACG	180	0.0	39.111107	26
GACGGTC	180	0.0	39.111107	29
GCGTAAG	300	0.0	38.866665	1
AAGCGAC	40	4.1270323E-7	38.500004	15
CGCACGA	40	4.1270323E-7	38.500004	39
TCGACAG	80	0.0	38.500004	1
CGTAAGG	490	0.0	38.163265	2
>>END_MODULE