FastQCFastQC Report
Thu 26 May 2016
SRR1546607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546607_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2414063
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78520.3252607740560209No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36670.15190158666116005No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA302.5288791E-644.00000418
ATCGGAT302.5288791E-644.00000410
TCGTTGC207.8582065E-444.016
TCGACTA207.8582065E-444.044
CTTACCG254.444301E-544.044
CGTTTTT84100.041.697981
GCGTAAG1600.041.251
TCGTTAG1350.040.740741
GTTTACG2000.040.6999971
TACGGGA10100.038.99014
GCACGAG1650.038.6666681
CGCTATA404.1270505E-738.516
CCGACCA404.1270505E-738.536
CGCGTAA404.1270505E-738.531
TTCGAAT404.1270505E-738.516
GCGTAAC404.1270505E-738.532
GGCGATA8050.038.260878
AGGGCGA22650.037.6865356
AGGGACC23900.037.6485336
GCGGGAT16950.037.6401185
Produced by FastQC (version 0.11.5)