##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546607_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2414063
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99194304373995	31.0	31.0	33.0	30.0	34.0
2	31.378853410205117	31.0	31.0	34.0	30.0	34.0
3	31.52763494573257	31.0	31.0	34.0	30.0	34.0
4	35.289378529060755	37.0	35.0	37.0	33.0	37.0
5	35.076539841752265	37.0	35.0	37.0	32.0	37.0
6	35.1464029729133	37.0	35.0	37.0	32.0	37.0
7	35.69984751847819	37.0	35.0	37.0	35.0	37.0
8	35.74869752777786	37.0	35.0	37.0	35.0	37.0
9	37.523620965981415	39.0	37.0	39.0	35.0	39.0
10	36.7539906787851	39.0	37.0	39.0	32.0	39.0
11	36.29742015846314	38.0	35.0	39.0	31.0	39.0
12	35.658699876515236	37.0	35.0	39.0	30.0	39.0
13	35.39102210671387	37.0	35.0	39.0	30.0	39.0
14	36.33111687640298	38.0	35.0	40.0	30.0	41.0
15	36.59142946973629	38.0	35.0	40.0	31.0	41.0
16	36.68602600677779	38.0	35.0	40.0	31.0	41.0
17	36.60179208247672	38.0	35.0	40.0	31.0	41.0
18	36.54567548568534	38.0	35.0	40.0	31.0	41.0
19	36.49501607870217	38.0	35.0	40.0	31.0	41.0
20	36.3528375191534	38.0	35.0	40.0	31.0	41.0
21	36.218930077632606	38.0	34.0	40.0	30.0	41.0
22	36.128598963655875	38.0	34.0	40.0	30.0	41.0
23	35.96409580031673	37.0	34.0	40.0	30.0	41.0
24	35.93521337264189	37.0	34.0	40.0	30.0	41.0
25	35.8082858649505	37.0	34.0	40.0	30.0	41.0
26	35.67778719942272	37.0	34.0	40.0	29.0	41.0
27	35.483838657069015	37.0	34.0	40.0	29.0	41.0
28	35.46708888707544	37.0	34.0	40.0	29.0	41.0
29	35.39451538754374	37.0	34.0	40.0	29.0	41.0
30	35.37991262034172	37.0	34.0	40.0	29.0	41.0
31	35.28140814883456	37.0	34.0	40.0	28.0	41.0
32	35.063857902631376	37.0	34.0	40.0	27.0	41.0
33	34.84364989646086	37.0	33.0	40.0	25.0	41.0
34	34.7660964109056	37.0	34.0	40.0	25.0	41.0
35	34.516114119639795	37.0	33.0	40.0	24.0	41.0
36	34.29544589349988	37.0	33.0	40.0	23.0	41.0
37	34.34137592929431	37.0	33.0	40.0	23.0	41.0
38	34.318069578134455	37.0	33.0	40.0	23.0	41.0
39	34.256022315904765	37.0	33.0	40.0	23.0	41.0
40	34.22389100864393	37.0	33.0	40.0	23.0	41.0
41	34.26729791227486	37.0	33.0	40.0	23.0	41.0
42	34.027728770955854	36.0	33.0	40.0	23.0	41.0
43	34.09052373529605	36.0	33.0	40.0	23.0	41.0
44	33.88909941455546	36.0	33.0	40.0	23.0	41.0
45	33.715036848665505	36.0	33.0	40.0	23.0	41.0
46	33.80020985367822	36.0	33.0	40.0	23.0	41.0
47	33.6606505298329	36.0	33.0	40.0	22.0	41.0
48	33.73500608724793	36.0	33.0	40.0	23.0	41.0
49	33.67302510332166	36.0	32.0	40.0	23.0	41.0
50	33.15741842694246	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	9.0
14	28.0
15	67.0
16	250.0
17	667.0
18	1610.0
19	3203.0
20	5444.0
21	8528.0
22	12435.0
23	17730.0
24	25040.0
25	34260.0
26	43480.0
27	51334.0
28	58571.0
29	66655.0
30	78126.0
31	93813.0
32	113585.0
33	142879.0
34	214522.0
35	286504.0
36	186970.0
37	224423.0
38	308815.0
39	435113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.367957671361516	24.905398077846353	30.936143754326213	14.790500496465917
2	29.03789172030722	26.82050965529897	30.355048729051397	13.786549895342418
3	27.274433185877918	26.62598283474789	32.143568746963105	13.956015232411085
4	23.822534871707987	29.753366005775327	31.502077617692663	14.922021504824027
5	22.062887339725602	33.45674905750181	30.847662219254428	13.632701383518159
6	20.41305467172978	41.79907483773207	27.25222995423069	10.535640536307461
7	89.59484487355964	3.75470731294088	5.697034418737208	0.9534133947622743
8	90.55877994899056	2.8763126728672783	5.146013173641284	1.4188942045008768
9	85.40294930165452	5.427116028040693	6.915105363861672	2.254829306443121
10	43.69355729324379	35.07373254136284	11.563948413939487	9.668761751453877
11	34.30457283012084	24.112999536466116	28.291018088591724	13.291409544821326
12	33.75114899652578	23.083034701248476	27.770609134890016	15.39520716733573
13	21.038680432117967	35.70925862332508	28.95612086345717	14.29594008109979
14	17.346398996215097	37.90041933454098	28.746805696454487	16.006375972789442
15	15.44118774033652	25.66482316327287	43.77089578855233	15.123093307838278
16	17.765443569616863	22.83660368432804	41.61511112178928	17.782841624265814
17	17.843154880382162	22.677452908229817	30.647046079576217	28.832346131811804
18	20.11455376268142	25.519880798471288	34.944282729986746	19.42128270886054
19	25.76863155601159	25.831678792144196	28.670834191154082	19.72885546069013
20	27.65797744300791	25.617102784807187	28.369475030270543	18.355444741914358
21	21.754693228801404	29.6983135899933	29.941927779018197	18.6050654021871
22	22.29444716231515	25.814322161434895	27.635981331058883	24.255249345191075
23	18.513767039219772	31.308503547753308	27.719284873675626	22.458444539351294
24	17.960923140779673	25.876085255438653	38.92479193790717	17.238199665874504
25	17.60202612773569	27.25330697666134	35.60702434029269	19.53764255531028
26	18.029231217246608	33.440386601343874	29.00483541647422	19.5255467649353
27	17.502732944417772	34.74080833847336	29.56923659407397	18.1872221230349
28	16.26096750581903	30.542574903803256	35.9780585676513	17.218399022726416
29	16.865964144266325	25.916266476889792	36.071096736083526	21.146672642760358
30	18.60610100067811	31.615289244729738	31.69784715643295	18.080762598159204
31	25.17096695488063	28.34884590832965	28.468105430554214	18.012081706235506
32	26.681076674469555	27.187028673236778	28.855543538010398	17.276351114283266
33	25.94708588798221	28.095455669549636	27.31494579884618	18.642512643621977
34	20.126898096694244	27.909089365107704	29.442562186653788	22.521450351544264
35	20.096410077118946	26.94859247666693	32.60362302060882	20.351374425605297
36	27.094114776623478	26.827303181399987	27.757104930567262	18.32147711140927
37	20.467320032658634	32.47404893741381	29.876519378326083	17.18211165160147
38	20.4206766766236	32.630465733495775	26.916944586781703	20.031913003098925
39	20.498470835268176	31.886988864830784	28.53981027007166	19.07473002982938
40	23.62212585172798	27.45723703151078	27.720817559442317	21.19981955731893
41	18.274626635676036	26.000812737695746	29.844581520863372	25.879979105764843
42	20.604184729230347	26.85468440550226	27.93796185103703	24.603169014230367
43	21.1134506431688	26.82353360289272	28.54921350437002	23.513802249568467
44	19.6444334717031	29.712439153410664	30.361842255152418	20.281285119733827
45	18.087100460924177	34.89411833908228	26.398358286424173	20.620422913569364
46	21.091703074857616	32.67930455833174	27.59865836144293	18.630334005367715
47	20.488363393995932	28.35746208777484	28.855129298613996	22.29904521961523
48	21.159886879505628	26.5229200729227	32.38614733749699	19.931045710074674
49	21.109639640721888	26.3920204236592	32.16784317559235	20.330496760026563
50	19.978766088540358	31.88752737604611	28.76407947928451	19.369627056129023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2579.0
1	3081.0
2	3583.0
3	10079.0
4	16575.0
5	13440.0
6	10305.0
7	11104.0
8	11903.0
9	12774.0
10	13645.0
11	14138.5
12	14632.0
13	14644.0
14	14656.0
15	14079.5
16	13503.0
17	13575.0
18	13647.0
19	14754.0
20	15861.0
21	15907.0
22	15953.0
23	17431.0
24	18909.0
25	22283.5
26	25658.0
27	31053.5
28	36449.0
29	43537.0
30	50625.0
31	57413.0
32	64201.0
33	74366.0
34	84531.0
35	94706.0
36	104881.0
37	110280.5
38	115680.0
39	129947.0
40	144214.0
41	169752.5
42	195291.0
43	215504.5
44	235718.0
45	235036.0
46	234354.0
47	218428.0
48	202502.0
49	188310.5
50	174119.0
51	153115.0
52	132111.0
53	118505.0
54	104899.0
55	91953.5
56	79008.0
57	73558.0
58	68108.0
59	63351.5
60	58595.0
61	52370.5
62	46146.0
63	38936.5
64	31727.0
65	26446.5
66	21166.0
67	17229.5
68	13293.0
69	11160.0
70	9027.0
71	7824.5
72	6622.0
73	5282.0
74	3942.0
75	3082.0
76	2222.0
77	1924.0
78	1626.0
79	1250.5
80	875.0
81	667.0
82	459.0
83	298.0
84	137.0
85	94.0
86	51.0
87	46.0
88	41.0
89	36.5
90	32.0
91	17.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2414063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.327403577123977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.93334738896712	16.451648152231076
2	6.495513407312033	2.640738449421027
3	2.1877813718003902	1.3341574464930137
4	1.1949167329431716	0.9715821868637725
5	0.8002549309968642	0.8133552473478377
6	0.601067639348754	0.7330886689314795
7	0.4728818878092255	0.6728722684467272
8	0.39908407639729926	0.6489874465705343
9	0.33817911697221825	0.6186873052844727
>10	4.103068494723807	19.776053939077315
>50	1.4622089929707025	21.452464041763474
>100	1.0061201715491872	32.67865035862742
>500	0.004543234837148387	0.552391899895234
>1k	8.26042697663343E-4	0.3257093055161578
>5k	2.0651067441583576E-4	0.3296132835304641
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7852	0.3252607740560209	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3667	0.15190158666116005	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021167633156218377	0.0
2	0.0	0.0	0.0	0.09999739029180266	0.0
3	0.0	0.0	0.0	0.16026922246851055	0.0
4	0.0	0.0	0.0	0.2792387771155931	0.0
5	0.0	0.0	0.0	0.4586458596979449	0.0
6	0.0	0.0	0.0	0.7927299328973602	0.0
7	0.0	0.0	0.0	0.9688645242481244	0.0
8	0.0	0.0	0.0	1.420344042388289	0.0
9	0.0	0.0	0.0	1.671207420850243	0.0
10	0.0	0.0	0.0	2.0653976304678046	0.0
11	0.0	0.0	0.0	2.380385267493019	0.0
12	0.0	0.0	0.0	2.643551556028157	0.0
13	0.0	0.0	0.0	2.780167708962028	0.0
14	0.0	0.0	0.0	2.8425521620603935	0.0
15	0.0	0.0	0.0	2.897563153902777	0.0
16	0.0	0.0	0.0	3.004809733631641	0.0
17	0.0	0.0	0.0	3.1241106797958462	0.0
18	2.0711969820174535E-4	0.0	0.0	3.293161777468111	0.0
19	2.0711969820174535E-4	0.0	0.0	3.3708730882334055	0.0
20	2.0711969820174535E-4	0.0	0.0	3.453596695695183	0.0
21	2.0711969820174535E-4	0.0	0.0	3.556866577218573	0.0
22	2.0711969820174535E-4	0.0	0.0	3.667799887575428	0.0
23	2.0711969820174535E-4	0.0	0.0	3.7914089234622295	0.0
24	2.0711969820174535E-4	0.0	0.0	3.885358418566541	0.0
25	2.0711969820174535E-4	0.0	0.0	3.9652651981327747	0.0
26	2.0711969820174535E-4	0.0	0.0	4.046456119827859	0.0
27	2.0711969820174535E-4	0.0	0.0	4.13439914368432	0.0
28	2.485436378420944E-4	0.0	0.0	4.218903980550632	0.0
29	2.485436378420944E-4	0.0	0.0	4.318155739928908	0.0
30	2.485436378420944E-4	0.0	0.0	4.456843089844797	0.0
31	2.485436378420944E-4	0.0	0.0	4.566616529891722	0.0
32	2.485436378420944E-4	0.0	0.0	4.6707977380872	0.0
33	2.485436378420944E-4	0.0	0.0	4.77402619567095	0.0
34	2.485436378420944E-4	0.0	0.0	4.8762604787033315	0.0
35	2.485436378420944E-4	0.0	0.0	5.004384724010931	0.0
36	2.485436378420944E-4	0.0	0.0	5.120330331064268	0.0
37	2.485436378420944E-4	0.0	0.0	5.241785322089772	0.0
38	2.485436378420944E-4	0.0	0.0	5.363033193417073	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGA	30	2.5288791E-6	44.000004	18
ATCGGAT	30	2.5288791E-6	44.000004	10
TCGTTGC	20	7.8582065E-4	44.0	16
TCGACTA	20	7.8582065E-4	44.0	44
CTTACCG	25	4.444301E-5	44.0	44
CGTTTTT	8410	0.0	41.69798	1
GCGTAAG	160	0.0	41.25	1
TCGTTAG	135	0.0	40.74074	1
GTTTACG	200	0.0	40.699997	1
TACGGGA	1010	0.0	38.9901	4
GCACGAG	165	0.0	38.666668	1
CGCTATA	40	4.1270505E-7	38.5	16
CCGACCA	40	4.1270505E-7	38.5	36
CGCGTAA	40	4.1270505E-7	38.5	31
TTCGAAT	40	4.1270505E-7	38.5	16
GCGTAAC	40	4.1270505E-7	38.5	32
GGCGATA	805	0.0	38.26087	8
AGGGCGA	2265	0.0	37.686535	6
AGGGACC	2390	0.0	37.648533	6
GCGGGAT	1695	0.0	37.640118	5
>>END_MODULE