FastQCFastQC Report
Thu 26 May 2016
SRR1546606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546606_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1502997
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53900.358616816933101No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40280.2679978735819167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24110.16041282850198638No Hit
GAGCGAGGGATATTCTCCATTCATTTCTTTTTCATAAGTATTTCTTGCTC15590.10372608860829396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG650.044.00000433
ACGCCTA207.857257E-444.021
AATCACG254.4434957E-544.044
CAACGCG254.4434957E-544.01
GACAACG302.5282388E-644.01
ACCGAAT302.5282388E-644.036
ATGCTCG207.857257E-444.012
GTTAGGT254.4434957E-544.027
TATGCGA254.4434957E-544.043
CGTTCGA302.5282388E-644.014
TATGATC207.857257E-444.015
ATCCGTC207.857257E-444.034
ATCCGAT207.857257E-444.031
CTAACGG502.7284841E-1144.02
TACGCAA207.857257E-444.015
TATCTAG207.857257E-444.01
TATTCGA408.314601E-944.011
CGTACTA254.4434957E-544.024
GCCTACT254.4434957E-544.036
ACCGTTC351.4464786E-744.033
Produced by FastQC (version 0.11.5)