##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546606_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1502997
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.960224804174594	31.0	31.0	33.0	30.0	34.0
2	31.3297764400062	31.0	31.0	34.0	30.0	34.0
3	31.47194505378254	31.0	31.0	34.0	30.0	34.0
4	35.23131716164437	37.0	35.0	37.0	33.0	37.0
5	35.012896898663136	37.0	35.0	37.0	32.0	37.0
6	35.068654162317024	37.0	35.0	37.0	32.0	37.0
7	35.613207478125375	37.0	35.0	37.0	35.0	37.0
8	35.656358595526136	37.0	35.0	37.0	35.0	37.0
9	37.41241599284629	39.0	37.0	39.0	35.0	39.0
10	36.725060662130396	39.0	37.0	39.0	32.0	39.0
11	36.271165544575275	38.0	35.0	39.0	32.0	39.0
12	35.10737879050989	37.0	34.0	39.0	30.0	39.0
13	34.57617613341876	35.0	33.0	39.0	27.0	39.0
14	35.41368944848193	37.0	33.0	40.0	27.0	41.0
15	35.87428052085267	37.0	34.0	40.0	30.0	41.0
16	36.087440626960664	37.0	34.0	40.0	31.0	41.0
17	36.02908788241094	37.0	34.0	40.0	31.0	41.0
18	35.969700538324425	36.0	34.0	40.0	30.0	41.0
19	35.865207981120385	36.0	34.0	40.0	30.0	41.0
20	35.658919478881195	36.0	34.0	40.0	30.0	41.0
21	35.482253790260394	35.0	34.0	40.0	30.0	41.0
22	35.35881508745526	35.0	34.0	40.0	29.0	41.0
23	35.247446934358486	35.0	34.0	40.0	29.0	41.0
24	35.1799431402724	35.0	34.0	40.0	29.0	41.0
25	35.04751107287639	35.0	34.0	40.0	29.0	41.0
26	34.88377288843557	35.0	34.0	39.0	29.0	41.0
27	34.697113167890556	35.0	33.0	39.0	27.0	41.0
28	34.730455882480136	35.0	33.0	39.0	27.0	41.0
29	34.682449133298334	35.0	34.0	39.0	27.0	41.0
30	34.62862600524153	35.0	33.0	39.0	27.0	41.0
31	34.410330825676965	35.0	33.0	39.0	26.0	41.0
32	34.117992916818864	35.0	33.0	39.0	25.0	41.0
33	33.82130436720765	35.0	33.0	39.0	23.0	41.0
34	33.72704336735203	35.0	33.0	39.0	23.0	41.0
35	33.40929023810427	35.0	32.0	39.0	23.0	41.0
36	33.05750577013793	35.0	32.0	39.0	21.0	41.0
37	33.071396682761176	35.0	32.0	39.0	21.0	41.0
38	33.06710658770443	35.0	32.0	39.0	21.0	41.0
39	32.994315357914886	35.0	32.0	39.0	21.0	41.0
40	32.86476553180079	35.0	31.0	39.0	19.0	41.0
41	32.909739008128426	35.0	32.0	39.0	19.0	41.0
42	32.68358486410818	35.0	32.0	39.0	18.0	41.0
43	32.712703352035966	35.0	32.0	39.0	18.0	41.0
44	32.54546416260312	35.0	31.0	39.0	18.0	41.0
45	32.33020824392863	35.0	31.0	38.0	18.0	41.0
46	32.40888637834939	35.0	31.0	38.0	18.0	41.0
47	32.26175967084432	35.0	31.0	38.0	18.0	40.0
48	32.31792145959041	35.0	31.0	38.0	18.0	40.0
49	32.288824927794266	35.0	31.0	38.0	18.0	40.0
50	31.73216646473679	35.0	30.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	13.0
15	65.0
16	208.0
17	611.0
18	1322.0
19	2635.0
20	4473.0
21	7196.0
22	10508.0
23	15043.0
24	20926.0
25	27967.0
26	33912.0
27	38678.0
28	43427.0
29	48598.0
30	56383.0
31	65928.0
32	80135.0
33	101844.0
34	165528.0
35	231171.0
36	99399.0
37	113434.0
38	146120.0
39	187464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.400726016086526	25.35733604258691	30.268257355137766	13.973680586188793
2	28.70750906355768	28.698127807307667	29.692607503541257	12.901755625593397
3	27.768585033769195	26.405708061958872	32.272386438562414	13.553320465709511
4	23.608629957345222	30.07544259902049	31.75329025939506	14.562637184239225
5	22.91155604435671	33.66147770088696	30.068922293258073	13.35804396149826
6	20.942091035444516	41.661427135250435	27.35554362383957	10.04093820546548
7	86.58067847108146	5.345253516806753	6.730885025053277	1.3431829870585237
8	86.6910579329167	4.5065958215485455	6.005467742117915	2.7968785034168397
9	82.02058952878815	6.472468008918182	8.633550166766799	2.8733922955268705
10	54.16091981554189	23.042095227069648	12.081527774173868	10.715457183214603
11	48.312937417706095	20.39797817294379	20.091324200913242	11.197760208436877
12	43.39117110679529	21.671101139922435	21.863383626181555	13.074344127100721
13	19.84215537356362	43.91459197856017	23.79372680051923	12.44952584735698
14	13.791444693502383	46.15152259119613	26.56691929524809	13.490113420053401
15	12.015060575636545	23.377026035314774	51.10030159740838	13.507611791640302
16	13.215062970850907	18.81846736886368	49.91666650033233	18.049803159953083
17	14.037286834238524	19.56337903535403	29.039512387582946	37.35982174282451
18	18.285864842045594	23.54861653083805	37.794952351867636	20.37056627524872
19	29.435321560854742	24.094791939039133	25.87916010477732	20.590726395328797
20	30.507446122646954	23.46924178824043	26.12899426944964	19.89431781966298
21	20.32858349018661	32.92455008226896	27.65521155398181	19.09165487356262
22	22.22965182232566	25.610763028801784	23.071503136732808	29.088082012139743
23	17.127712164428804	34.03493154011618	23.56884278544801	25.268513510007008
24	16.775748720722664	24.07915651195578	42.4634247440281	16.68167002329346
25	15.15059577630561	25.230389681416533	39.109259699121154	20.509754843156706
26	14.556383013405883	37.34019429180498	27.639842261827535	20.46358043296161
27	16.102959620012548	38.755300243446925	26.87749875748255	18.264241379057975
28	14.216661776437345	30.782829240510797	39.2367383301497	15.763770652902167
29	14.68213176739541	25.0341817049535	38.26514623781684	22.018540289834245
30	17.966037191025663	33.982769094016824	29.56260059068648	18.48859312427104
31	29.276106339533612	27.435849838688963	24.82792713491777	18.460116686859653
32	31.872319106425362	25.07110792636313	27.140306999947438	15.916265967264073
33	30.503720233639854	27.212096897066328	23.82772553770899	18.456457331584826
34	20.974958699185695	26.509301083102628	26.040105203137465	26.475635014574213
35	19.85639359226931	24.79146664963403	33.04870202668402	22.303437731412636
36	33.31576842801416	23.481350927513496	25.67210712995435	17.530773514517996
37	20.033439853838697	33.93692735248307	29.156079486519264	16.873553307158964
38	20.682609479593104	34.218498107447985	24.31115963637985	20.787732776579062
39	20.868704328751157	33.80745270948645	26.864990415815864	18.458852545946534
40	27.15654123062122	24.34662211567954	25.51116203159421	22.985674622105034
41	16.76789774031485	22.578887383008748	29.101056089932314	31.552158786744084
42	20.3625156936441	24.39845189311755	25.73371736603599	29.50531504720236
43	20.650007950781006	24.229988483010946	27.81329570185436	27.306707864353687
44	19.01327813694904	28.44676336679315	30.454751406689436	22.085207089568375
45	16.443811930429668	37.94997594805579	24.043960167585166	21.562251953929383
46	21.8151466702861	33.460346228235984	25.926066386027387	18.79844071545053
47	21.380814466030206	26.062460537180048	27.02107855172033	25.535646445069414
48	21.693988743823176	23.598716431237055	33.713706680718595	20.993588144221178
49	22.450876482122055	22.087602303930083	34.195943172208594	21.26557804173927
50	19.859254542756904	33.01230807513255	26.548822120070763	20.579615262039777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3212.0
1	3334.0
2	3456.0
3	7063.5
4	10671.0
5	8733.5
6	6796.0
7	7713.0
8	8630.0
9	9766.5
10	10903.0
11	11412.5
12	11922.0
13	12092.0
14	12262.0
15	11911.5
16	11561.0
17	11194.5
18	10828.0
19	10714.5
20	10601.0
21	10304.5
22	10008.0
23	9685.5
24	9363.0
25	9278.5
26	9194.0
27	11147.0
28	13100.0
29	15627.0
30	18154.0
31	21226.0
32	24298.0
33	26171.5
34	28045.0
35	32386.0
36	36727.0
37	40216.5
38	43706.0
39	54409.0
40	65112.0
41	94606.5
42	124101.0
43	148258.0
44	172415.0
45	173739.0
46	175063.0
47	164024.0
48	152985.0
49	135489.5
50	117994.0
51	103945.5
52	89897.0
53	76534.0
54	63171.0
55	56593.0
56	50015.0
57	46009.5
58	42004.0
59	39785.5
60	37567.0
61	36207.5
62	34848.0
63	31411.5
64	27975.0
65	23142.0
66	18309.0
67	16006.0
68	13703.0
69	11264.5
70	8826.0
71	7260.5
72	5695.0
73	5104.0
74	4513.0
75	3497.0
76	2481.0
77	1901.0
78	1321.0
79	1105.0
80	889.0
81	636.0
82	383.0
83	296.0
84	209.0
85	127.0
86	45.0
87	34.0
88	23.0
89	17.0
90	11.0
91	7.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1502997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.50388051692653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.57442873607064	18.807489585469252
2	6.451580960563391	2.9037121416359333
3	2.0861583864020767	1.4083997720092973
4	1.054476538035194	0.9491925607938536
5	0.6634078222013845	0.7464625182407199
6	0.4934517365967862	0.6662747352746387
7	0.3829368553771275	0.6032295667254116
8	0.28155316524182966	0.5068831031811686
9	0.23951479144148452	0.48510110237721715
>10	2.721935881204195	14.413990300456131
>50	1.0681406940688638	17.580105564250463
>100	0.9689730677998736	38.258168072453294
>500	0.011947879997528867	1.6926269326347851
>1k	0.0011947879997528865	0.6160567823993409
>5k	2.986969999382216E-4	0.36230726209846675
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5390	0.358616816933101	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4028	0.2679978735819167	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2411	0.16041282850198638	No Hit
GAGCGAGGGATATTCTCCATTCATTTCTTTTTCATAAGTATTTCTTGCTC	1559	0.10372608860829396	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04344652717204359	0.0
2	0.0	0.0	0.0	0.203526687012682	0.0
3	0.0	0.0	0.0	0.3103133273053772	0.0
4	0.0	0.0	0.0	0.5975394495132059	0.0
5	0.0	0.0	0.0	0.9662028600190153	0.0
6	0.0	0.0	0.0	1.746909674470408	0.0
7	0.0	0.0	0.0	2.104395417954926	0.0
8	0.0	0.0	0.0	3.08390502442786	0.0
9	0.0	0.0	0.0	3.6085900371058623	0.0
10	0.0	0.0	0.0	4.426622275360496	0.0
11	0.0	0.0	0.0	5.011719916939288	0.0
12	0.0	0.0	0.0	5.506930486221862	0.0
13	0.0	0.0	0.0	5.747849130770055	0.0
14	0.0	0.0	0.0	5.844456110025503	0.0
15	0.0	0.0	0.0	5.9323471703536335	0.0
16	0.0	0.0	0.0	6.119240424298917	0.0
17	0.0	0.0	0.0	6.28344567554027	0.0
18	0.0	0.0	0.0	6.556034376648789	0.0
19	0.0	0.0	0.0	6.66508316383865	0.0
20	0.0	0.0	0.0	6.795023542961164	0.0
21	0.0	0.0	0.0	6.940399747970222	0.0
22	0.0	0.0	0.0	7.079521782145939	0.0
23	0.0	0.0	0.0	7.242529426206439	0.0
24	0.0	0.0	0.0	7.361425205772201	0.0
25	0.0	0.0	0.0	7.462290343892902	0.0
26	0.0	0.0	0.0	7.5655506963753085	0.0
27	0.0	0.0	0.0	7.670807060825803	0.0
28	0.0	0.0	0.0	7.76588376423905	0.0
29	0.0	0.0	0.0	7.866283166233865	0.0
30	0.0	0.0	0.0	8.026097191145425	0.0
31	0.0	0.0	0.0	8.149716865702327	0.0
32	0.0	0.0	0.0	8.259231389018074	0.0
33	0.0	0.0	0.0	8.357634779044801	0.0
34	0.0	0.0	0.0	8.466883167431472	0.0
35	0.0	0.0	0.0	8.613856182015	0.0
36	0.0	0.0	0.0	8.725300183566567	0.0
37	0.0	0.0	0.0	8.845327036580912	0.0
38	0.0	0.0	0.0	8.958767050100565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACCG	65	0.0	44.000004	33
ACGCCTA	20	7.857257E-4	44.0	21
AATCACG	25	4.4434957E-5	44.0	44
CAACGCG	25	4.4434957E-5	44.0	1
GACAACG	30	2.5282388E-6	44.0	1
ACCGAAT	30	2.5282388E-6	44.0	36
ATGCTCG	20	7.857257E-4	44.0	12
GTTAGGT	25	4.4434957E-5	44.0	27
TATGCGA	25	4.4434957E-5	44.0	43
CGTTCGA	30	2.5282388E-6	44.0	14
TATGATC	20	7.857257E-4	44.0	15
ATCCGTC	20	7.857257E-4	44.0	34
ATCCGAT	20	7.857257E-4	44.0	31
CTAACGG	50	2.7284841E-11	44.0	2
TACGCAA	20	7.857257E-4	44.0	15
TATCTAG	20	7.857257E-4	44.0	1
TATTCGA	40	8.314601E-9	44.0	11
CGTACTA	25	4.4434957E-5	44.0	24
GCCTACT	25	4.4434957E-5	44.0	36
ACCGTTC	35	1.4464786E-7	44.0	33
>>END_MODULE