FastQCFastQC Report
Thu 26 May 2016
SRR1546605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546605_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3076481
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128200.41670987079068583No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40810.13265155871269804No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG1800.041.5555571
CGTTTTT118700.041.034541
TATTGCG1750.038.9714281
TAATGCG2050.038.6341481
ACGGGTA2450.038.6122445
GTATCGC404.1275416E-738.50000428
CGGTCTA1150.038.26086831
TACGGGA10000.038.064
CGTTAGG4050.037.481482
GGGCGAT76200.037.4461947
TAGTACG1000.037.41
AGGGCGA34950.036.8869826
CACTACG301.3016029E-436.66666410
CGCGTAG301.3016029E-436.66666433
GGCGATA17550.036.353288
GGCGATT22350.036.0268448
AACGGGA11650.035.879834
TATGGGA32600.035.8343544
ATGGGAC21000.035.8285755
TGGGCGA24550.035.75566
Produced by FastQC (version 0.11.5)