##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546605_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3076481 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.944693628857127 31.0 31.0 33.0 30.0 34.0 2 31.314974153911564 31.0 31.0 34.0 30.0 34.0 3 31.47455485666903 31.0 31.0 34.0 30.0 34.0 4 35.254428680040604 37.0 35.0 37.0 33.0 37.0 5 35.048040927280226 37.0 35.0 37.0 32.0 37.0 6 35.116559146635396 37.0 35.0 37.0 32.0 37.0 7 35.64618536568241 37.0 35.0 37.0 35.0 37.0 8 35.67471471463663 37.0 35.0 37.0 35.0 37.0 9 37.43116827310164 39.0 37.0 39.0 35.0 39.0 10 36.68936294422101 39.0 37.0 39.0 32.0 39.0 11 36.276639121125726 38.0 35.0 39.0 31.0 39.0 12 35.709347140450404 37.0 35.0 39.0 30.0 39.0 13 35.512520961449134 37.0 35.0 39.0 30.0 39.0 14 36.408298312260015 38.0 35.0 40.0 30.0 41.0 15 36.62191250327891 38.0 35.0 40.0 31.0 41.0 16 36.689869692028005 38.0 35.0 40.0 31.0 41.0 17 36.59651530433635 38.0 35.0 40.0 31.0 41.0 18 36.51776461483104 38.0 35.0 40.0 31.0 41.0 19 36.47051420112785 38.0 35.0 40.0 31.0 41.0 20 36.33018113877511 38.0 35.0 40.0 30.0 41.0 21 36.205066112873766 38.0 34.0 40.0 30.0 41.0 22 36.116950502863496 38.0 34.0 40.0 30.0 41.0 23 35.92751848621851 38.0 34.0 40.0 30.0 41.0 24 35.86878969835991 37.0 34.0 40.0 30.0 41.0 25 35.69157521206859 37.0 34.0 40.0 29.0 41.0 26 35.540144405247425 37.0 34.0 40.0 29.0 41.0 27 35.34822870675944 37.0 34.0 40.0 28.0 41.0 28 35.30409354063945 37.0 34.0 40.0 27.0 41.0 29 35.177090318451505 37.0 34.0 40.0 27.0 41.0 30 35.12724050627974 37.0 34.0 40.0 27.0 41.0 31 35.02104709894194 37.0 34.0 40.0 27.0 41.0 32 34.80983077743695 37.0 33.0 40.0 25.0 41.0 33 34.59516961099386 37.0 33.0 40.0 24.0 41.0 34 34.4687921037055 37.0 33.0 40.0 24.0 41.0 35 34.23872372363099 37.0 33.0 40.0 23.0 41.0 36 34.014960274417426 36.0 33.0 40.0 23.0 41.0 37 34.08403529877155 37.0 33.0 40.0 23.0 41.0 38 34.01948329926302 36.0 33.0 40.0 23.0 41.0 39 33.924588190208226 36.0 33.0 40.0 23.0 41.0 40 33.86140236198436 36.0 33.0 40.0 22.0 41.0 41 33.87681087580258 36.0 33.0 40.0 22.0 41.0 42 33.619943370363735 36.0 32.0 40.0 21.0 41.0 43 33.649417630078 36.0 33.0 40.0 21.0 41.0 44 33.44665154766111 35.0 32.0 40.0 20.0 41.0 45 33.17970564420843 35.0 32.0 40.0 20.0 41.0 46 33.26643460499187 35.0 32.0 40.0 20.0 41.0 47 33.12917095863748 35.0 32.0 40.0 20.0 41.0 48 33.17810283892538 35.0 32.0 40.0 20.0 41.0 49 33.09552862507521 35.0 32.0 39.0 20.0 41.0 50 32.595187488562416 35.0 31.0 39.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 2.0 12 4.0 13 14.0 14 32.0 15 137.0 16 348.0 17 1061.0 18 2428.0 19 4578.0 20 7991.0 21 12430.0 22 18466.0 23 26010.0 24 35939.0 25 48327.0 26 60593.0 27 71141.0 28 79425.0 29 89929.0 30 104000.0 31 123984.0 32 148736.0 33 184286.0 34 268305.0 35 336616.0 36 251241.0 37 299294.0 38 392121.0 39 509042.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.293623461350805 23.681407426211962 28.968454542706425 14.056514569730805 2 30.810949263135377 26.564344132143187 28.74771532799975 13.876991276721684 3 28.46502221206632 26.11724239480107 31.8769074146728 13.540827978459804 4 24.88671309850443 29.102178755532705 31.069328885827673 14.941779260135199 5 22.471973660815717 33.60323044413406 30.014552340807565 13.910243554242655 6 21.218788609453462 41.571912844577945 26.906033224323505 10.303265321645087 7 88.09295425520261 3.862594958330638 6.746084243653707 1.2983665428130386 8 88.21088119835618 3.2608034959422794 6.492060246755953 2.036255058945594 9 82.46928877506475 5.792722269372052 9.048097485406217 2.689891470156975 10 43.12989418754739 30.34948046160532 14.582667664776736 11.937957686070547 11 33.918785781547165 25.211727294919093 26.528914041724942 14.340572881808795 12 31.481618121483606 23.317192597646468 28.356066557862704 16.84512272300723 13 22.71319081769073 32.01671000080937 29.514207953827764 15.755891227672137 14 18.66151619333908 33.456406849254066 30.857625969411156 17.024450987995703 15 17.61509334853685 24.450045360267136 41.10732359471747 16.827537696478544 16 20.147337168667708 22.27050971548337 39.217274541919814 18.364878573929108 17 20.467475664566106 23.272628694927743 30.37132359991822 25.88857204058793 18 22.391849648998317 24.08160492458754 33.93094252816773 19.595602898246405 19 26.687471822514098 25.60782920486101 28.206512570693594 19.498186401931296 20 27.885333925351723 25.821027336102514 27.69511659587691 18.59852214266885 21 24.079947186412006 28.69408262232076 29.3073157285873 17.918654462679925 22 23.68082234215001 25.356145544211067 27.80563247424574 23.15739963939319 23 20.79983591642529 29.360070808173365 27.95681169492027 21.883281580481075 24 20.496469830302868 25.74379623992477 35.35523216298102 18.40450176679134 25 20.189300697777753 26.54841684379003 32.801080195197045 20.46120226323517 26 19.47257272188582 30.82183832762172 28.951097048868498 20.754491901623968 27 19.44500876163383 31.438354405569218 29.494542628412134 19.622094204384815 28 18.639055466294117 29.687100294134762 33.10636405685587 18.56748018271525 29 19.17674771922856 26.198179023371182 33.211094103945385 21.413979153454875 30 21.18547132259227 29.245784388071954 30.426939090473827 19.141805198861945 31 25.50215652233835 27.285882799211176 27.68764052175196 19.52432015669851 32 26.454965917228158 26.936132548843954 27.732431957161445 18.876469576766443 33 25.63363791292714 27.348844345211297 26.377344765009113 20.64017297685245 34 21.26266991409991 28.280720732551252 28.488003013833012 21.96860633951583 35 20.533915210267836 27.609239257450312 30.024726302551517 21.83211922973033 36 26.264065989681068 27.970008590984307 26.391094240464994 19.374831178869627 37 20.856751593785237 31.902683618068828 27.39688624763163 19.843678540514308 38 21.21137754466873 31.40204668905805 26.4604917111466 20.92608405512662 39 21.692999241666048 30.47530603959524 27.022204915291205 20.80948980344751 40 24.441724164719368 26.652691825498025 27.52011145201287 21.385472557769738 41 19.057455579930448 26.083177500527388 29.03193616342828 25.827430756113884 42 21.431011600591717 27.012713551619527 27.02018962574448 24.536085222044278 43 21.237608813446272 26.278855614580426 28.592830574932854 23.890704997040448 44 20.475374299402464 28.947066469775045 29.017601603910443 21.559957626912045 45 19.770315500079473 31.49292974668135 26.345717720993562 22.39103703224561 46 21.794478821744715 30.524843156840557 27.016061532640702 20.664616488774023 47 20.940971194036305 27.976932085717415 28.331363008580258 22.750733711666022 48 22.050355584838652 26.741364565553955 30.144408497890936 21.063871351716458 49 22.268364407256215 26.048462512851533 30.338136331737463 21.34503674815479 50 20.847682790824972 30.012569555930945 28.096094206335092 21.043653446908987 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3120.0 1 4179.0 2 5238.0 3 15454.0 4 25670.0 5 19800.0 6 13930.0 7 14965.5 8 16001.0 9 17240.0 10 18479.0 11 19256.0 12 20033.0 13 19887.0 14 19741.0 15 19596.0 16 19451.0 17 19534.5 18 19618.0 19 19559.5 20 19501.0 21 19987.0 22 20473.0 23 22975.5 24 25478.0 25 27876.5 26 30275.0 27 34237.0 28 38199.0 29 46346.0 30 54493.0 31 63833.0 32 73173.0 33 84245.0 34 95317.0 35 103616.0 36 111915.0 37 118560.0 38 125205.0 39 137252.0 40 149299.0 41 183645.0 42 217991.0 43 235058.0 44 252125.0 45 252551.5 46 252978.0 47 247665.0 48 242352.0 49 235659.5 50 228967.0 51 204789.5 52 180612.0 53 161757.5 54 142903.0 55 132577.5 56 122252.0 57 117300.0 58 112348.0 59 106945.5 60 101543.0 61 94287.5 62 87032.0 63 79465.0 64 71898.0 65 60404.0 66 48910.0 67 42004.5 68 35099.0 69 29841.0 70 24583.0 71 21682.5 72 18782.0 73 16074.0 74 13366.0 75 10520.0 76 7674.0 77 6209.0 78 4744.0 79 3826.5 80 2909.0 81 2357.0 82 1805.0 83 1146.5 84 488.0 85 405.0 86 322.0 87 226.0 88 130.0 89 76.0 90 22.0 91 21.5 92 21.0 93 17.5 94 14.0 95 8.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3076481.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.591030823381168 #Duplication Level Percentage of deduplicated Percentage of total 1 81.26296732119165 17.545512322312657 2 6.473061284653523 2.795201314371791 3 2.1842075851492266 1.4147787988685971 4 1.2012269953434633 1.0374291632935306 5 0.805910217914245 0.8700216177932149 6 0.5884334960714037 0.7622931450712572 7 0.4670741944570008 0.7059229330528924 8 0.37221619798330063 0.6429225122895353 9 0.32672644436746046 0.6348924658036414 >10 4.10593647233472 20.94327369523283 >50 1.3195648937544526 20.51016921096265 >100 0.8873479223490071 30.776266829992128 >500 0.0044137788138432004 0.6164468371150578 >1k 7.609963472143448E-4 0.32358611893341727 >5k 0.0 0.0 >10k+ 1.5219926944286897E-4 0.4212830349067136 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12820 0.41670987079068583 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 4081 0.13265155871269804 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03250467010847784 0.0 2 0.0 0.0 0.0 0.11373384070956395 0.0 3 0.0 0.0 0.0 0.17149463949232907 0.0 4 0.0 0.0 0.0 0.31549032807288585 0.0 5 0.0 0.0 0.0 0.511070928115597 0.0 6 0.0 0.0 0.0 0.9727022529961993 0.0 7 0.0 0.0 0.0 1.2059557656946363 0.0 8 0.0 0.0 0.0 1.8992803791084685 0.0 9 0.0 0.0 0.0 2.225692276337803 0.0 10 3.2504670108477834E-5 0.0 0.0 2.7011705906846166 0.0 11 3.2504670108477834E-5 0.0 0.0 3.0115576855504713 0.0 12 3.2504670108477834E-5 0.0 0.0 3.290090203710018 0.0 13 3.2504670108477834E-5 0.0 0.0 3.43740136864164 0.0 14 3.2504670108477834E-5 0.0 0.0 3.5053036244982496 0.0 15 3.2504670108477834E-5 0.0 0.0 3.56423459140492 0.0 16 3.2504670108477834E-5 0.0 0.0 3.6731252362683207 0.0 17 3.2504670108477834E-5 0.0 0.0 3.78952445992678 0.0 18 3.2504670108477834E-5 0.0 0.0 3.9682676408532997 0.0 19 3.2504670108477834E-5 0.0 0.0 4.046506381804406 0.0 20 3.2504670108477834E-5 0.0 0.0 4.136186766633696 0.0 21 6.500934021695567E-5 0.0 0.0 4.239584122248764 0.0 22 6.500934021695567E-5 0.0 0.0 4.354813177783318 0.0 23 6.500934021695567E-5 0.0 0.0 4.485221914258531 0.0 24 6.500934021695567E-5 0.0 0.0 4.588749288554033 0.0 25 6.500934021695567E-5 0.0 0.0 4.681875168414822 0.0 26 6.500934021695567E-5 0.0 0.0 4.771620562584329 0.0 27 6.500934021695567E-5 0.0 0.0 4.866014124579349 0.0 28 6.500934021695567E-5 0.0 0.0 4.962000415409684 0.0 29 6.500934021695567E-5 0.0 0.0 5.069883415499722 0.0 30 6.500934021695567E-5 0.0 0.0 5.22558078531933 0.0 31 6.500934021695567E-5 0.0 0.0 5.348643466350028 0.0 32 6.500934021695567E-5 0.0 0.0 5.47586674515461 0.0 33 6.500934021695567E-5 0.0 0.0 5.600554659690731 0.0 34 6.500934021695567E-5 0.0 0.0 5.721634555844811 0.0 35 6.500934021695567E-5 0.0 0.0 5.8684581507248055 0.0 36 6.500934021695567E-5 0.0 0.0 5.998151784457632 0.0 37 6.500934021695567E-5 0.0 0.0 6.141074818924609 0.0 38 6.500934021695567E-5 0.0 0.0 6.301225328549079 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTAG 180 0.0 41.555557 1 CGTTTTT 11870 0.0 41.03454 1 TATTGCG 175 0.0 38.971428 1 TAATGCG 205 0.0 38.634148 1 ACGGGTA 245 0.0 38.612244 5 GTATCGC 40 4.1275416E-7 38.500004 28 CGGTCTA 115 0.0 38.260868 31 TACGGGA 1000 0.0 38.06 4 CGTTAGG 405 0.0 37.48148 2 GGGCGAT 7620 0.0 37.446194 7 TAGTACG 100 0.0 37.4 1 AGGGCGA 3495 0.0 36.886982 6 CACTACG 30 1.3016029E-4 36.666664 10 CGCGTAG 30 1.3016029E-4 36.666664 33 GGCGATA 1755 0.0 36.35328 8 GGCGATT 2235 0.0 36.026844 8 AACGGGA 1165 0.0 35.87983 4 TATGGGA 3260 0.0 35.834354 4 ATGGGAC 2100 0.0 35.828575 5 TGGGCGA 2455 0.0 35.7556 6 >>END_MODULE