FastQCFastQC Report
Thu 26 May 2016
SRR1546603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546603_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1178200
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76120.6460702766932609No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36400.3089458496010864No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36020.3057205907316245No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG29440.24987268714988967No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC27710.23518927177049737No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT27420.2327278900016975No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC25790.21889322695637414No Hit
GAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT25160.21354608725173996No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC21500.18248175182481752No Hit
AAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT16980.14411814632490239No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC16900.14343914445764724No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT13510.11466644033271091No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT13120.11135630622984213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA207.033358E-445.00000418
AGCGTTG207.033358E-445.0000041
CGGGTAT207.033358E-445.0000046
CGGGTAC551.8189894E-1245.0000046
CGTGGAC207.033358E-445.00000425
CCTTACG207.033358E-445.00000444
CTACTTT207.033358E-445.00000421
CGAGTTA207.033358E-445.00000429
CGTGCTA207.033358E-445.00000424
CTATCCG207.033358E-445.00000418
TTGTCGA207.033358E-445.00000416
CCGTGTT207.033358E-445.00000419
CGCGCCA207.033358E-445.00000438
TATGCGC207.033358E-445.00000417
GTTCGCG207.033358E-445.0000041
TGATTCG406.8157533E-945.00000415
TACGAGT207.033358E-445.00000442
GGACGTA207.033358E-445.00000428
CGTACTG207.033358E-445.00000430
CGGCTAA207.033358E-445.00000433