##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546603_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1178200
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.411442878967918	31.0	31.0	33.0	30.0	34.0
2	31.75253182821253	31.0	31.0	34.0	30.0	34.0
3	31.812736377525038	31.0	31.0	34.0	30.0	34.0
4	35.58114751315566	37.0	35.0	37.0	33.0	37.0
5	35.49402478356816	37.0	35.0	37.0	33.0	37.0
6	35.55252843320319	37.0	35.0	37.0	33.0	37.0
7	35.91585723985741	37.0	35.0	37.0	35.0	37.0
8	34.573887285690034	37.0	35.0	37.0	32.0	37.0
9	36.87362586997114	39.0	37.0	39.0	33.0	39.0
10	36.92086063486675	39.0	37.0	39.0	32.0	39.0
11	36.80478102189781	39.0	35.0	39.0	33.0	39.0
12	36.03289594296384	37.0	35.0	39.0	32.0	39.0
13	35.62944491597352	37.0	35.0	39.0	31.0	39.0
14	36.60332286538788	38.0	35.0	41.0	31.0	41.0
15	36.943338142929896	38.0	35.0	41.0	32.0	41.0
16	37.122786453912745	38.0	35.0	41.0	32.0	41.0
17	37.01409607876422	38.0	35.0	41.0	32.0	41.0
18	36.93654303174334	38.0	35.0	40.0	32.0	41.0
19	36.82681293498557	37.0	35.0	40.0	32.0	41.0
20	36.581175521982686	37.0	35.0	40.0	32.0	41.0
21	36.48377270412494	37.0	35.0	40.0	32.0	41.0
22	36.38906212867085	37.0	35.0	40.0	31.0	41.0
23	36.33073077575963	37.0	35.0	40.0	32.0	41.0
24	36.20261755219827	37.0	35.0	40.0	31.0	41.0
25	36.12944067221185	36.0	35.0	40.0	31.0	41.0
26	36.01356391105075	36.0	35.0	40.0	31.0	41.0
27	35.90971736547276	36.0	35.0	40.0	31.0	41.0
28	35.96201408928874	36.0	35.0	40.0	31.0	41.0
29	35.94542522491937	36.0	35.0	40.0	31.0	41.0
30	35.89542946868104	36.0	35.0	40.0	31.0	41.0
31	35.7088142929893	36.0	35.0	40.0	30.0	41.0
32	35.41071804447462	36.0	34.0	40.0	30.0	41.0
33	35.16881089797997	36.0	34.0	40.0	29.0	41.0
34	34.959373620777455	36.0	34.0	40.0	27.0	41.0
35	34.771407231369885	36.0	34.0	40.0	26.0	41.0
36	34.54664573077576	36.0	34.0	40.0	24.0	41.0
37	34.43767187234765	36.0	34.0	40.0	24.0	41.0
38	34.35087081989475	35.0	34.0	40.0	24.0	41.0
39	34.295864878628414	36.0	34.0	40.0	23.0	41.0
40	34.082366321507386	35.0	34.0	40.0	23.0	41.0
41	34.06016720420981	36.0	34.0	40.0	23.0	41.0
42	34.00794941436089	35.0	34.0	40.0	22.0	41.0
43	33.85432354438975	35.0	33.0	40.0	22.0	41.0
44	33.730935325072146	35.0	33.0	40.0	21.0	41.0
45	33.61141402138856	35.0	33.0	40.0	21.0	41.0
46	33.55040485486335	35.0	33.0	40.0	20.0	41.0
47	33.49655746053302	35.0	33.0	40.0	20.0	41.0
48	33.499040061110165	35.0	33.0	40.0	20.0	41.0
49	33.508462909523004	35.0	33.0	40.0	20.0	41.0
50	33.34534968596164	35.0	33.0	39.0	20.0	41.0
51	32.95292564929554	35.0	33.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	5.0
14	19.0
15	28.0
16	99.0
17	282.0
18	651.0
19	1305.0
20	2297.0
21	3930.0
22	6253.0
23	8805.0
24	12738.0
25	17450.0
26	21852.0
27	23998.0
28	24624.0
29	26507.0
30	30680.0
31	36886.0
32	46194.0
33	60658.0
34	109292.0
35	205682.0
36	80855.0
37	94861.0
38	134899.0
39	227137.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.93914445764726	25.112374809030726	29.07867934136819	14.869801391953827
2	29.739093532507216	28.89543371244271	28.774656255304702	12.590816499745374
3	27.55457477508063	26.65693430656934	32.77431675437107	13.01417416397895
4	23.659650314038362	29.339246307927347	32.57222882362926	14.428874554405024
5	23.516211169580718	31.69309115600068	30.952384994058736	13.838312680359872
6	19.42132065863181	41.27669326090647	28.193345781700902	11.108640298760822
7	79.65260566966559	6.201154303174334	11.418774401629603	2.72746562553047
8	75.82371414021388	8.98226107621796	9.307163469699542	5.886861313868613
9	73.30826684773383	7.100237650653539	13.834153793922932	5.757341707689696
10	49.084960108640296	19.756832456289256	16.913597012391783	14.244610422678663
11	44.05270751994568	21.237735528772706	22.51145815650993	12.198098794771687
12	38.23162451196741	20.914870140892887	25.365472755050078	15.48803259208963
13	19.909268375488033	40.201748429808184	28.351043965370902	11.53793922933288
14	13.280597521643184	43.90315735868274	28.53191308776099	14.284332031913088
15	10.327873026650822	24.906976744186046	51.550925140044136	13.214225089118994
16	11.36131386861314	19.714479714819216	48.86139874384654	20.0628076727211
17	12.35664573077576	21.83092853505347	29.982430826684777	35.829994907486
18	17.807672721099983	24.43532507214395	36.238414530639965	21.51858767611611
19	24.078254965201154	27.364963503649637	27.58606348667459	20.970718044474623
20	26.13766762858598	24.872941775589883	26.238669156340176	22.75072143948396
21	18.66576133084366	31.957222882362927	29.66661008317773	19.710405703615685
22	18.912663384824306	25.717789848922084	26.743846545578	28.62570022067561
23	15.916313019860803	32.98260057715159	24.28017314547615	26.820913257511457
24	17.810813104736038	26.074775080631476	37.25819046002377	18.856221354608728
25	14.425055168901716	27.412154133423865	35.32235613647938	22.840434561195046
26	13.244355796978441	33.72899337973179	31.473349176710236	21.553301646579527
27	16.754625700220675	33.63647937531829	29.44517059921915	20.163724325241894
28	14.449414360889493	30.217450347988457	38.246647428280426	17.086487862841622
29	14.74113053810898	25.062468171787472	37.3152266168732	22.88117467323035
30	17.770412493634357	29.633084365982004	34.26141571889323	18.33508742149041
31	26.698438295705312	27.02130368358513	25.65124766593108	20.629010354778476
32	28.405703615684942	27.937362077745714	26.964691902902732	16.69224240366661
33	25.779154642675266	27.801137328127652	27.030215583092854	19.389492446104224
34	20.15625530470209	27.809709726701747	29.75131556611781	22.282719402478357
35	19.887879816669496	26.612374809030726	30.44669835342047	23.05304702087931
36	28.136649125785095	22.857324732643015	30.340349685961638	18.665676455610253
37	20.911135630622983	30.861483619079955	30.24435579697844	17.983024953318623
38	20.57808521473434	30.75564420302156	24.98472245798676	23.681548124257343
39	19.85494822610762	31.659141062637925	27.0295365812256	21.45637413002886
40	21.887879816669496	26.150314038363604	27.848497708368697	24.113308436598203
41	15.814547615005942	25.673909353250725	27.913342386691564	30.598200645051776
42	23.322101510779156	25.111271430996435	25.92199966049907	25.644627397725344
43	22.77355287727041	25.021133933118318	28.353250721439483	23.852062468171788
44	21.42743167543711	29.435409947377355	27.553810897979968	21.583347479205568
45	17.586233237141403	32.8706501442879	25.383805805465965	24.159310813104735
46	23.836105924291292	29.280852147343406	27.77609913427262	19.106942794092685
47	21.987353590222373	26.00458326260397	28.483788830419282	23.524274316754372
48	23.174927856051607	22.000679001867255	30.78585978611441	24.038533355966727
49	22.53191308776099	20.407231369886265	32.153963673400106	24.90689186895264
50	20.038448480733322	27.609319300628076	29.434476319809878	22.91775589882872
51	18.14029876082159	29.463249023934818	25.471736547275505	26.924715667968087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4374.0
1	4118.5
2	3863.0
3	8190.5
4	12518.0
5	9127.0
6	5736.0
7	6724.0
8	7712.0
9	8811.0
10	9910.0
11	10443.5
12	10977.0
13	10851.0
14	10725.0
15	10527.0
16	10329.0
17	9858.5
18	9388.0
19	8936.0
20	8484.0
21	8160.0
22	7836.0
23	7300.0
24	6764.0
25	6950.5
26	7216.0
27	7295.0
28	7382.5
29	7470.0
30	8994.0
31	10518.0
32	12298.5
33	14079.0
34	16033.0
35	17987.0
36	18961.5
37	19936.0
38	26905.0
39	33874.0
40	54488.5
41	75103.0
42	98437.0
43	121771.0
44	137792.0
45	153813.0
46	152787.0
47	151761.0
48	134231.5
49	116702.0
50	99543.5
51	82385.0
52	72469.0
53	62553.0
54	54548.5
55	46544.0
56	41156.0
57	35768.0
58	32554.0
59	29340.0
60	25794.5
61	22249.0
62	20037.0
63	17825.0
64	15589.5
65	13354.0
66	10112.0
67	6870.0
68	5892.5
69	4915.0
70	4577.0
71	4239.0
72	3433.0
73	2627.0
74	2281.0
75	1326.0
76	717.0
77	612.0
78	507.0
79	364.0
80	221.0
81	134.5
82	48.0
83	36.0
84	24.0
85	16.0
86	8.0
87	5.5
88	3.0
89	3.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1178200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.123182535066746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6433032863324	21.066597319796095
2	8.137555282361552	4.251576840606549
3	2.83974214505385	2.225493072232912
4	1.4451374970354667	1.510063624933079
5	0.8760473018630868	1.1442571787961067
6	0.6332827441975968	0.9926016433789082
7	0.4413990854964536	0.8071524216864775
8	0.34369564666825353	0.7182739291538074
9	0.2696926485131966	0.6340707256928247
>10	2.5540090618648166	15.249253845693259
>50	1.1153530799442106	21.275388225861537
>100	0.6907279563840371	25.755485867773242
>500	0.00435684785686192	0.7851465501245464
>1k	0.005362274285368516	2.918209724437041
>5k	3.3514214283553227E-4	0.6664290298336359
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7612	0.6460702766932609	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3640	0.3089458496010864	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3602	0.3057205907316245	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG	2944	0.24987268714988967	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	2771	0.23518927177049737	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	2742	0.2327278900016975	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2579	0.21889322695637414	No Hit
GAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	2516	0.21354608725173996	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2150	0.18248175182481752	No Hit
AAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	1698	0.14411814632490239	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC	1690	0.14343914445764724	No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT	1351	0.11466644033271091	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT	1312	0.11135630622984213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6975046681378373E-4	0.0	0.0	0.2422339161432694	0.0
2	1.6975046681378373E-4	0.0	0.0	0.8603802410456629	0.0
3	1.6975046681378373E-4	0.0	0.0	1.2099813274486504	0.0
4	1.6975046681378373E-4	0.0	0.0	2.034713970463419	0.0
5	1.6975046681378373E-4	0.0	0.0	3.311916482770328	0.0
6	1.6975046681378373E-4	0.0	0.0	4.928789679171618	0.0
7	1.6975046681378373E-4	0.0	0.0	5.777117637073502	0.0
8	1.6975046681378373E-4	0.0	0.0	8.21464946528603	0.0
9	1.6975046681378373E-4	0.0	0.0	9.325157019181804	0.0
10	1.6975046681378373E-4	0.0	0.0	11.349091835002547	0.0
11	1.6975046681378373E-4	0.0	0.0	12.760566966559159	0.0
12	1.6975046681378373E-4	0.0	0.0	14.128331352911221	0.0
13	1.6975046681378373E-4	0.0	0.0	14.61195043286369	0.0
14	1.6975046681378373E-4	0.0	0.0	14.804107961296893	0.0
15	1.6975046681378373E-4	0.0	0.0	15.07044644372772	0.0
16	1.6975046681378373E-4	0.0	0.0	15.603717535223222	0.0
17	1.6975046681378373E-4	0.0	0.0	16.141911390256322	0.0
18	1.6975046681378373E-4	0.0	0.0	17.051349516211168	0.0
19	1.6975046681378373E-4	0.0	0.0	17.406127991851978	0.0
20	1.6975046681378373E-4	0.0	0.0	17.760057715158716	0.0
21	1.6975046681378373E-4	0.0	0.0	18.115854693600408	0.0
22	1.6975046681378373E-4	0.0	0.0	18.443303344084196	0.0
23	1.6975046681378373E-4	0.0	0.0	18.79876082159226	0.0
24	1.6975046681378373E-4	0.0	0.0	19.065353929723308	0.0
25	1.6975046681378373E-4	0.0	0.0	19.263367849261584	0.0
26	1.6975046681378373E-4	0.0	0.0	19.465201154303173	0.0
27	1.6975046681378373E-4	0.0	0.0	19.697674418604652	0.0
28	1.6975046681378373E-4	0.0	0.0	19.886861313868614	0.0
29	1.6975046681378373E-4	0.0	0.0	20.09039212357834	0.0
30	1.6975046681378373E-4	0.0	0.0	20.416313019860805	0.0
31	2.5462570022067563E-4	0.0	0.0	20.67229672381599	0.0
32	2.5462570022067563E-4	0.0	0.0	20.88007129519606	0.0
33	2.5462570022067563E-4	0.0	0.0	21.096078764216603	0.0
34	2.5462570022067563E-4	0.0	0.0	21.30071295196062	0.0
35	2.5462570022067563E-4	0.0	0.0	21.561619419453404	0.0
36	2.5462570022067563E-4	0.0	0.0	21.773468002037006	0.0
37	2.5462570022067563E-4	0.0	0.0	21.975640808012223	0.0
38	2.5462570022067563E-4	0.0	0.0	22.167204209811576	0.0
39	2.5462570022067563E-4	0.0	0.0	22.38117467323035	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	20	7.033358E-4	45.000004	18
AGCGTTG	20	7.033358E-4	45.000004	1
CGGGTAT	20	7.033358E-4	45.000004	6
CGGGTAC	55	1.8189894E-12	45.000004	6
CGTGGAC	20	7.033358E-4	45.000004	25
CCTTACG	20	7.033358E-4	45.000004	44
CTACTTT	20	7.033358E-4	45.000004	21
CGAGTTA	20	7.033358E-4	45.000004	29
CGTGCTA	20	7.033358E-4	45.000004	24
CTATCCG	20	7.033358E-4	45.000004	18
TTGTCGA	20	7.033358E-4	45.000004	16
CCGTGTT	20	7.033358E-4	45.000004	19
CGCGCCA	20	7.033358E-4	45.000004	38
TATGCGC	20	7.033358E-4	45.000004	17
GTTCGCG	20	7.033358E-4	45.000004	1
TGATTCG	40	6.8157533E-9	45.000004	15
TACGAGT	20	7.033358E-4	45.000004	42
GGACGTA	20	7.033358E-4	45.000004	28
CGTACTG	20	7.033358E-4	45.000004	30
CGGCTAA	20	7.033358E-4	45.000004	33
>>END_MODULE