FastQCFastQC Report
Thu 26 May 2016
SRR1546602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546602_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3198307
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT548381.714594627720228No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT55610.17387323981093747No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT41960.13119441004256313No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC41940.13113187695865344No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39260.12275244371475283No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT37910.11853146055084768TruSeq Adapter, Index 16 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35360.11055849235236018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT372300.043.9907341
CGACGGT2600.041.5384628
TAACGCG650.041.538461
TACGGGA5200.039.3754
CACGACG2750.039.27272826
AGGGCGA31500.039.2142876
CTTGCGG3200.038.671882
CGAGGGT7400.038.614874
TCACGAC2800.038.5714325
AGTACGG2450.038.571432
AAGGGCG12950.038.5714265
GTTTTTT416200.038.5235442
CGTTCGG3700.038.31081445
TATAGCG1000.038.251
TGCGGGT7500.038.1000024
ATACGAA4600.037.66304419
GCGGGTA5450.037.568815
TCTTGCG1500.037.51
AGCGGGT7900.037.3101274
CGGTCTA2900.037.2413831