##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546602_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3198307
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31437507406262	31.0	31.0	33.0	30.0	34.0
2	31.658598752402444	31.0	31.0	34.0	30.0	34.0
3	31.60136065737279	31.0	31.0	34.0	30.0	34.0
4	35.47809544236998	37.0	35.0	37.0	33.0	37.0
5	35.44060685856611	37.0	35.0	37.0	33.0	37.0
6	35.49967498429638	37.0	35.0	37.0	33.0	37.0
7	35.95263400292718	37.0	35.0	37.0	35.0	37.0
8	34.649391693793	37.0	35.0	37.0	33.0	37.0
9	36.922560904878736	39.0	37.0	39.0	33.0	39.0
10	36.70070290312969	39.0	37.0	39.0	32.0	39.0
11	36.694729430289215	39.0	35.0	39.0	32.0	39.0
12	36.526451025495675	39.0	35.0	39.0	32.0	39.0
13	36.49235329816681	39.0	35.0	39.0	32.0	39.0
14	37.64018995049568	40.0	36.0	41.0	32.0	41.0
15	37.7886910168411	40.0	36.0	41.0	33.0	41.0
16	37.81140084425917	40.0	36.0	41.0	33.0	41.0
17	37.727974831684385	40.0	36.0	41.0	32.0	41.0
18	37.53605641984963	39.0	36.0	41.0	32.0	41.0
19	37.32371532814079	39.0	36.0	41.0	32.0	41.0
20	37.055357412531066	38.0	35.0	41.0	32.0	41.0
21	36.98562051735496	38.0	35.0	41.0	32.0	41.0
22	36.901815241626274	38.0	35.0	41.0	32.0	41.0
23	36.823671711314766	38.0	35.0	41.0	32.0	41.0
24	36.6669663043604	38.0	35.0	40.0	31.0	41.0
25	36.57348684788546	38.0	35.0	40.0	31.0	41.0
26	36.473651841427355	38.0	35.0	40.0	31.0	41.0
27	36.35724869438737	38.0	35.0	40.0	31.0	41.0
28	36.31520457542069	38.0	35.0	40.0	31.0	41.0
29	36.21973969353161	38.0	35.0	40.0	30.0	41.0
30	36.093386282179914	38.0	35.0	40.0	30.0	41.0
31	35.87734354456905	38.0	35.0	40.0	30.0	41.0
32	35.545055555955074	38.0	35.0	40.0	27.0	41.0
33	35.12348595678901	38.0	34.0	40.0	24.0	41.0
34	34.72388454266586	38.0	34.0	40.0	22.0	41.0
35	34.43772189473994	38.0	34.0	40.0	19.0	41.0
36	34.249197466034374	38.0	34.0	40.0	18.0	41.0
37	34.17277015621077	38.0	34.0	40.0	18.0	41.0
38	34.0813198983087	38.0	33.0	40.0	18.0	41.0
39	33.99364038536638	38.0	33.0	40.0	17.0	41.0
40	33.894790587645275	38.0	33.0	40.0	16.0	41.0
41	33.80852932504603	38.0	33.0	40.0	15.0	41.0
42	33.73368472757618	38.0	33.0	40.0	15.0	41.0
43	33.63354205834524	38.0	33.0	40.0	15.0	41.0
44	33.515253851490804	37.0	33.0	40.0	15.0	41.0
45	33.412512932623414	37.0	33.0	40.0	14.0	41.0
46	33.33695983531287	37.0	33.0	40.0	13.0	41.0
47	33.27821719428435	37.0	33.0	40.0	13.0	41.0
48	33.19166671617202	37.0	33.0	40.0	12.0	41.0
49	33.13615672291622	37.0	33.0	40.0	12.0	41.0
50	33.02447951369271	37.0	32.0	40.0	11.0	41.0
51	32.68779982659576	36.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	4.0
12	3.0
13	12.0
14	33.0
15	107.0
16	304.0
17	884.0
18	2011.0
19	4028.0
20	6963.0
21	11423.0
22	17318.0
23	26515.0
24	41681.0
25	66036.0
26	89691.0
27	88921.0
28	77349.0
29	73736.0
30	78794.0
31	91247.0
32	111143.0
33	139496.0
34	207578.0
35	313386.0
36	250667.0
37	300014.0
38	426086.0
39	772238.0
40	637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.355935186959854	23.237387780472606	25.611518844188502	20.79515818837904
2	37.7902121341072	23.817413400277086	26.085738486017757	12.306635979597957
3	26.17344113620112	23.04200315979673	37.90814953036091	12.876406173641241
4	22.376182148868136	25.734427620613033	37.903772214487226	13.985618016031607
5	19.966876225453028	29.82696783016765	36.986255540822064	13.219900403557258
6	17.508262965375117	38.30711060570483	34.39244575333137	9.792180675588678
7	78.28694993945234	3.657966542924116	15.941340215307662	2.1137433023158816
8	75.61728752117918	5.993202028448176	14.910794992475706	3.4787154578969437
9	72.23793713361475	5.303712245259757	18.530678887298812	3.927671733826678
10	34.30039705381628	22.447188465647606	28.243161147444567	15.009253333091538
11	28.5661445258382	25.525817252690253	30.16805453635314	15.739983685118409
12	23.516160268542073	21.996449996826446	36.22122579227072	18.266163942360755
13	19.133310216936646	28.13016386481973	37.728022982159	15.008502936084621
14	16.974511827663825	31.01578428837507	36.56271896350163	15.44698492045948
15	15.842975674317694	25.405347266538204	43.94818883865745	14.803488220486653
16	16.28680423736683	23.22581915994931	42.62692730872927	17.860449293954584
17	16.902630047709614	24.832731817177027	35.85328112654601	22.411357008567347
18	18.921729527528157	24.99860082224752	37.95576847375815	18.123901176466173
19	21.155974082538044	28.234156383361576	33.17455141110594	17.43531812299445
20	21.85884594568314	25.49695823446592	33.63079279131115	19.013403028539788
21	19.309559713936153	28.76171674576581	34.13762343639932	17.791100103898717
22	18.866231415558293	25.952261618412493	33.19199814151675	21.98950882451247
23	17.303998646784063	29.1788436819855	32.504165485051935	21.0129921861785
24	17.61816486034643	26.396027648377718	37.83776854442053	18.148038946855323
25	17.34908500028296	28.120283637562	35.57985521715082	18.950776145004216
26	16.563075402079914	30.047053018987857	34.63688757833442	18.752984000597817
27	18.572607320060268	28.698527064475048	33.63251245111867	19.09635316434601
28	17.81423734494531	27.742271145327823	36.85756245413589	17.585929055590974
29	17.782720670654818	26.615112307855377	34.86863518730378	20.733531834186024
30	18.899217617320662	28.79632880770983	33.55834821360176	18.74610536136775
31	21.296079457037738	27.208738873410216	31.002933739631622	20.492247929920424
32	20.920662087785818	27.387270827972426	31.328637307175327	20.36342977706643
33	20.616563700732918	27.84029175435629	29.831345146041325	21.711799398869463
34	17.586773252223757	29.044491351205497	30.389077721431995	22.979657675138753
35	18.83252608333096	27.142766469885476	30.30206293517164	23.722644511611925
36	21.163634385316982	28.03783376642705	29.297719074497852	21.500812773758117
37	18.16620480773109	29.120031316568422	31.271544601565765	21.44221927413472
38	18.529459492162573	30.019288329731946	30.342678173170995	21.108574004934486
39	18.236054262458232	28.804739507495686	30.762963030128127	22.196243199917955
40	20.007772862329976	27.25717074689828	30.965320089659937	21.76973630111181
41	17.42168591070213	26.65644667631969	31.567169755748903	24.354697657229277
42	19.957933994453942	28.04002242436389	29.969574528023735	22.032469053158437
43	20.04413585062347	27.472784820218948	31.204540402156518	21.278538927001065
44	20.195747312562553	29.346588679573287	29.10158405681506	21.3560799510491
45	18.510918432783345	30.214016353026775	28.98602291774992	22.28904229643996
46	21.40063477333477	29.578899086297845	27.8696510372519	21.15081510311549
47	20.218728220899372	27.673453486485194	29.838286318355305	22.269531974260133
48	20.198154836293075	26.835197496675583	30.536937198336496	22.429710468694843
49	20.08446968974523	25.595228975830025	30.860076909439897	23.460224424984844
50	18.862291831271982	27.629398928870806	31.032449355237006	22.475859884620206
51	18.260504698266928	27.90751481955922	29.58074381227318	24.25123666990067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4712.0
1	4685.5
2	4659.0
3	40758.0
4	76857.0
5	52520.0
6	28183.0
7	29529.5
8	30876.0
9	34688.5
10	38501.0
11	40951.5
12	43402.0
13	44072.5
14	44743.0
15	44152.5
16	43562.0
17	41696.5
18	39831.0
19	38151.0
20	36471.0
21	34594.5
22	32718.0
23	32279.5
24	31841.0
25	32568.0
26	34249.5
27	35204.0
28	40016.0
29	44828.0
30	50109.0
31	55390.0
32	61944.0
33	68498.0
34	76167.5
35	83837.0
36	91116.0
37	98395.0
38	111350.5
39	124306.0
40	141930.0
41	159554.0
42	185164.0
43	210774.0
44	230651.0
45	250528.0
46	279423.5
47	308319.0
48	312552.0
49	316785.0
50	291838.5
51	266892.0
52	229246.0
53	191600.0
54	164861.5
55	138123.0
56	122816.5
57	107510.0
58	92824.5
59	78139.0
60	67771.5
61	57404.0
62	47939.0
63	38474.0
64	33066.0
65	27658.0
66	22044.5
67	16431.0
68	13133.5
69	9836.0
70	8399.5
71	6963.0
72	6262.0
73	5561.0
74	4476.0
75	2610.0
76	1829.0
77	1474.0
78	1119.0
79	908.0
80	697.0
81	472.0
82	247.0
83	227.0
84	207.0
85	154.0
86	101.0
87	67.5
88	34.0
89	23.5
90	13.0
91	9.0
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3198307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.60350183364865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.16069316128223	24.225944183141987
2	7.664334238071192	4.691109338170158
3	2.9704097644244043	2.727148220167503
4	1.6247059148246552	1.9888676177390456
5	1.044168653576789	1.597760865218785
6	0.7918694372667403	1.4540386665241807
7	0.5718338806390724	1.2250083450276192
8	0.47019313539641683	1.15116451850186
9	0.39318289784988436	1.0829496181777403
>10	4.249767723268535	29.33052359181152
>50	0.8648930474541051	18.2437963651841
>100	0.18866269970978994	8.144528005417314
>500	0.002642723118101342	0.5713121737690716
>1k	0.0024313052686530698	1.6119026257200642
>5k	1.0570892472404651E-4	0.17990186853508763
>10k+	1.0570892472404651E-4	1.7740439968939283
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54838	1.714594627720228	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	5561	0.17387323981093747	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	4196	0.13119441004256313	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	4194	0.13113187695865344	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3926	0.12275244371475283	No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	3791	0.11853146055084768	TruSeq Adapter, Index 16 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3536	0.11055849235236018	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.379962586455896E-5	0.0	0.0	0.12559769903264445	0.0
2	9.379962586455896E-5	0.0	0.0	0.4029319261721905	0.0
3	9.379962586455896E-5	0.0	0.0	0.6729185159523461	0.0
4	9.379962586455896E-5	0.0	0.0	1.0763507067958142	0.0
5	9.379962586455896E-5	0.0	0.0	1.8551690003492474	0.0
6	9.379962586455896E-5	0.0	0.0	3.1239027397932717	0.0
7	9.379962586455896E-5	0.0	0.0	3.9121635290170707	0.0
8	9.379962586455896E-5	0.0	0.0	5.649363866570657	0.0
9	9.379962586455896E-5	0.0	0.0	6.599178878075182	0.0
10	9.379962586455896E-5	0.0	0.0	7.951738216500167	0.0
11	9.379962586455896E-5	0.0	0.0	8.791151068362105	0.0
12	9.379962586455896E-5	0.0	0.0	9.55277276384037	0.0
13	9.379962586455896E-5	0.0	0.0	9.953859964037223	0.0
14	9.379962586455896E-5	0.0	0.0	10.126107343666508	0.0
15	1.2506616781941197E-4	0.0	0.0	10.304232833183306	0.0
16	1.2506616781941197E-4	0.0	0.0	10.647132998802178	0.0
17	1.2506616781941197E-4	0.0	0.0	11.01714125629591	0.0
18	1.2506616781941197E-4	0.0	0.0	11.601481658890156	0.0
19	1.2506616781941197E-4	0.0	0.0	11.861869420290173	0.0
20	1.2506616781941197E-4	0.0	0.0	12.119286860204477	0.0
21	1.2506616781941197E-4	0.0	0.0	12.411535227856488	0.0
22	1.2506616781941197E-4	0.0	0.0	12.72692083655509	0.0
23	1.2506616781941197E-4	0.0	0.0	13.089237524727926	0.0
24	1.2506616781941197E-4	0.0	0.0	13.370542602695739	0.0
25	1.2506616781941197E-4	0.0	0.0	13.617579550681032	0.0
26	1.2506616781941197E-4	0.0	0.0	13.847201034797472	0.0
27	1.2506616781941197E-4	0.0	0.0	14.092612122601114	0.0
28	1.5633270977426494E-4	0.0	0.0	14.344870583092868	0.0
29	1.5633270977426494E-4	0.0	0.0	14.614888439414978	0.0
30	1.5633270977426494E-4	0.0	0.0	14.988367283065697	0.0
31	1.5633270977426494E-4	0.0	0.0	15.270454024582381	0.0
32	1.5633270977426494E-4	0.0	0.0	15.543911200519524	0.0
33	1.5633270977426494E-4	0.0	0.0	15.826029208578163	0.0
34	1.5633270977426494E-4	0.0	0.0	16.101800108619965	0.0
35	1.5633270977426494E-4	0.0	0.0	16.39701879775769	0.0
36	1.5633270977426494E-4	0.0	0.0	16.67901173964851	0.0
37	1.5633270977426494E-4	0.0	0.0	16.967132923762478	0.0
38	1.5633270977426494E-4	0.0	0.0	17.247718871265327	0.0
39	1.5633270977426494E-4	0.0	0.0	17.54687714468936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	37230	0.0	43.990734	1
CGACGGT	260	0.0	41.53846	28
TAACGCG	65	0.0	41.53846	1
TACGGGA	520	0.0	39.375	4
CACGACG	275	0.0	39.272728	26
AGGGCGA	3150	0.0	39.214287	6
CTTGCGG	320	0.0	38.67188	2
CGAGGGT	740	0.0	38.61487	4
TCACGAC	280	0.0	38.57143	25
AGTACGG	245	0.0	38.57143	2
AAGGGCG	1295	0.0	38.571426	5
GTTTTTT	41620	0.0	38.523544	2
CGTTCGG	370	0.0	38.310814	45
TATAGCG	100	0.0	38.25	1
TGCGGGT	750	0.0	38.100002	4
ATACGAA	460	0.0	37.663044	19
GCGGGTA	545	0.0	37.56881	5
TCTTGCG	150	0.0	37.5	1
AGCGGGT	790	0.0	37.310127	4
CGGTCTA	290	0.0	37.24138	31
>>END_MODULE