FastQCFastQC Report
Thu 26 May 2016
SRR1546601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546601_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2611617
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441171.6892599489128766No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT30000.11487136130604143No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC27470.10518387650256526No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT280650.043.0197751
TATTACG850.042.352941
CTATACG501.0822987E-940.51
GGGCGAT56100.038.262037
AGGGCGA25950.037.8901756
CACGACC9700.037.8092827
GCGCGAC11650.037.6609469
TAGGGCG12700.037.381895
TCAGACG11950.037.28033422
ACACGAC9850.037.23350526
CGTTCGG7400.037.09459745
CGACCCG12150.037.03703732
GGCGATT13050.036.896558
GTTTTTT325100.036.8748052
CGCTGAA12200.036.7008237
CGCGACC12150.036.66666410
ACCCGCT12050.036.5975134
GTTGATC18750.036.4816
AACACGT9400.036.3829841
TAGGGAC20050.036.3591045
Produced by FastQC (version 0.11.5)