##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546601_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2611617
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503380855615507	31.0	31.0	33.0	30.0	34.0
2	31.86398962788188	31.0	31.0	34.0	30.0	34.0
3	31.83654035028873	31.0	31.0	34.0	30.0	34.0
4	35.67927801051992	37.0	35.0	37.0	33.0	37.0
5	35.64300316623762	37.0	35.0	37.0	33.0	37.0
6	35.692920133388625	37.0	35.0	37.0	33.0	37.0
7	36.03558102126001	37.0	35.0	37.0	35.0	37.0
8	34.71221009818821	37.0	35.0	37.0	33.0	37.0
9	37.01399898989783	39.0	37.0	39.0	34.0	39.0
10	36.989166864819765	39.0	37.0	39.0	32.0	39.0
11	36.94225531538507	39.0	37.0	39.0	33.0	39.0
12	36.77058389495856	39.0	35.0	39.0	33.0	39.0
13	36.7420046660747	39.0	35.0	39.0	33.0	39.0
14	37.87434260077186	40.0	36.0	41.0	33.0	41.0
15	37.98963285964213	40.0	36.0	41.0	33.0	41.0
16	38.00703051021647	40.0	36.0	41.0	33.0	41.0
17	37.87956197252507	40.0	36.0	41.0	33.0	41.0
18	37.707699865638794	39.0	36.0	41.0	33.0	41.0
19	37.53916826242133	39.0	36.0	41.0	33.0	41.0
20	37.281962094748195	39.0	35.0	41.0	32.0	41.0
21	37.213568069131114	39.0	35.0	41.0	32.0	41.0
22	37.13311791124043	39.0	35.0	41.0	32.0	41.0
23	37.050874611399756	39.0	35.0	41.0	32.0	41.0
24	36.9134149456065	38.0	35.0	41.0	32.0	41.0
25	36.82347449874924	38.0	35.0	41.0	32.0	41.0
26	36.72671720240755	38.0	35.0	41.0	31.0	41.0
27	36.61036017149529	38.0	35.0	41.0	31.0	41.0
28	36.589364749884844	38.0	35.0	41.0	31.0	41.0
29	36.49792867790338	38.0	35.0	41.0	31.0	41.0
30	36.3751878625388	38.0	35.0	40.0	31.0	41.0
31	36.175981010998164	38.0	35.0	40.0	30.0	41.0
32	35.87976529483458	38.0	35.0	41.0	29.0	41.0
33	35.51371736361036	38.0	35.0	41.0	26.0	41.0
34	35.11372073317029	38.0	35.0	41.0	23.0	41.0
35	34.851960681830455	38.0	34.0	41.0	22.0	41.0
36	34.67679219426125	38.0	34.0	41.0	21.0	41.0
37	34.61924049353332	38.0	34.0	41.0	21.0	41.0
38	34.479001323700984	38.0	34.0	40.0	20.0	41.0
39	34.39476806897795	38.0	34.0	40.0	19.0	41.0
40	34.2558078003015	38.0	34.0	40.0	18.0	41.0
41	34.15097772759176	38.0	34.0	40.0	18.0	41.0
42	34.06333126181978	38.0	33.0	40.0	18.0	41.0
43	33.97213259065169	37.0	33.0	40.0	18.0	41.0
44	33.839040716919826	37.0	33.0	40.0	18.0	41.0
45	33.737116889651126	37.0	33.0	40.0	18.0	41.0
46	33.68084370717452	37.0	33.0	40.0	18.0	41.0
47	33.611919358772745	37.0	33.0	40.0	18.0	41.0
48	33.55518630794638	36.0	33.0	40.0	18.0	41.0
49	33.49248492409109	36.0	33.0	40.0	18.0	41.0
50	33.39664544992623	36.0	33.0	40.0	18.0	41.0
51	33.06145234925336	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	2.0
12	5.0
13	19.0
14	29.0
15	72.0
16	191.0
17	604.0
18	1365.0
19	2673.0
20	4762.0
21	7708.0
22	11869.0
23	17774.0
24	27046.0
25	42564.0
26	60234.0
27	68260.0
28	65414.0
29	61473.0
30	63661.0
31	72449.0
32	86478.0
33	108378.0
34	169280.0
35	241341.0
36	202156.0
37	255183.0
38	377848.0
39	662207.0
40	565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.50277510063688	22.10588306018838	24.511672270474577	20.87966956870016
2	38.318941866284376	23.648299118898368	25.229886311813715	12.802872703003542
3	27.423508117767653	23.789705764666106	35.904460722992695	12.882325394573554
4	23.847256316680433	25.697872237774526	35.828683914984474	14.62618753056057
5	21.986608296698943	29.116750273872473	34.664539249055274	14.23210218037331
6	19.315121627711875	38.00009725775257	32.52693637696492	10.157844737570631
7	79.7969610398462	3.465554099241964	14.86588577115251	1.871599089759333
8	77.09981976683412	6.229205890450245	13.980840222743227	2.6901341199724156
9	74.41228939771797	5.01409663055494	16.53986782901168	4.0337461427154135
10	35.70925598967995	27.65198725540537	22.850058029182687	13.78869872573199
11	27.69575324406297	24.44397474821155	31.83782308048998	16.0224489272355
12	25.3841968405015	21.60596289578449	34.79250594555021	18.217334318163804
13	22.903013726744774	24.288821829540854	35.38325106629341	17.42491337742096
14	19.1774291559597	27.42615015907769	35.88550694837719	17.51091373658542
15	18.271094115255032	23.282740156768774	40.89466411039597	17.55150161758022
16	21.788799812529938	21.81885781873835	38.29692485536738	18.095417513364325
17	20.658925102723718	22.1651566826223	35.12620725014426	22.04971096450973
18	22.279913172567035	22.31249834872418	36.36440565366208	19.043182825046706
19	24.455117270258235	24.667820740943256	32.07652576928394	18.800536219514576
20	25.424440107412384	24.378000296368114	31.248303254267373	18.949256341952132
21	23.61230609235581	24.916249204994454	33.980288840208964	17.49115586244078
22	22.156541330524345	21.699774507517755	33.8765600009496	22.267124161008294
23	20.6376739008821	25.5483863062616	32.76188660128955	21.05205319156676
24	20.793133143182942	24.62792208811629	35.82600358322066	18.752941185480108
25	21.954061410995564	24.891092376868432	33.30051841445357	19.85432779768243
26	19.419922599676752	25.85183815237839	33.09769388084087	21.63054536710398
27	20.038504880309784	24.23150102024914	34.28695708444232	21.443037014998755
28	19.23448193207503	25.53318499611543	35.53916979403947	19.693163277770058
29	20.60531846744756	24.14036974027968	34.99536876961668	20.25894302265608
30	21.911865330942476	23.886618903154634	35.634091828932036	18.56742393697085
31	23.992492007825035	23.925445423276077	32.34115109527929	19.7409114736196
32	23.824473496688068	24.257768271534456	32.30672797734124	19.611030254436237
33	23.36150362017095	24.6389114483479	30.586108146791812	21.413476784689333
34	20.25802405176563	26.862667841417785	32.64578994546291	20.233518161353675
35	20.86098382726104	26.868487990390626	31.66666475214398	20.603863430204353
36	23.035154082700487	28.67219810561809	29.079991438254538	19.212656373426885
37	21.766821092066717	28.64945357607949	29.27347310114768	20.310252230706112
38	21.781677788128963	29.800579487727337	28.340602775981317	20.077139948162383
39	21.77922719908777	27.592445599795067	28.21485692580497	22.413470275312193
40	22.54063287227798	26.38828740967761	29.883746353312908	21.187333364731504
41	20.70839636899285	26.068753572977965	29.93394513820365	23.288904919825534
42	22.321994381258815	27.273983895800953	28.682115333144175	21.721906389796054
43	21.882037067456675	26.156477002562013	29.768185763839032	22.19330016614228
44	22.154129031936918	26.547154502363863	29.73919989033614	21.55951657536308
45	21.10711486408612	26.988566853409207	29.434254716522368	22.4700635659823
46	22.41239814260667	26.562585555232637	29.362881310697546	21.662134991463144
47	21.22631304666802	27.167153529786336	30.414873237538277	21.191660186007365
48	21.485003352329226	26.371248157750543	30.68164282894467	21.462105660975556
49	21.836203394295563	25.593454170347336	30.543797195377426	22.026545239979676
50	20.784556081538756	25.976052384403992	31.220198061201167	22.019193472856088
51	20.68748978123515	26.716474888928964	30.4185108306463	22.177524499189584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3036.0
1	4029.5
2	5023.0
3	40242.0
4	75461.0
5	51956.5
6	28452.0
7	28248.5
8	28045.0
9	28664.0
10	29283.0
11	29166.5
12	29050.0
13	28375.0
14	27700.0
15	26355.5
16	25011.0
17	23716.0
18	22421.0
19	21044.0
20	19667.0
21	19419.0
22	19171.0
23	19036.0
24	18901.0
25	20543.0
26	23427.5
27	24670.0
28	26879.5
29	29089.0
30	33061.5
31	37034.0
32	43653.0
33	50272.0
34	55849.0
35	61426.0
36	68092.0
37	74758.0
38	82724.0
39	90690.0
40	103169.0
41	115648.0
42	134061.5
43	152475.0
44	173040.0
45	193605.0
46	197245.0
47	200885.0
48	198501.5
49	196118.0
50	183653.5
51	171189.0
52	164824.0
53	158459.0
54	146485.0
55	134511.0
56	128796.5
57	123082.0
58	113825.0
59	104568.0
60	96929.5
61	89291.0
62	79509.0
63	69727.0
64	63563.0
65	57399.0
66	48847.5
67	40296.0
68	34056.0
69	27816.0
70	23716.5
71	19617.0
72	17005.0
73	14393.0
74	11957.0
75	7616.0
76	5711.0
77	4501.5
78	3292.0
79	2377.0
80	1462.0
81	1097.0
82	732.0
83	500.0
84	268.0
85	179.5
86	91.0
87	78.5
88	66.0
89	40.5
90	15.0
91	15.0
92	15.0
93	12.0
94	9.0
95	10.0
96	11.0
97	5.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2611617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.54685475744449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.57509751567999	21.369499440590292
2	6.262116978354449	3.4500325375371426
3	2.5746438971549788	2.1277002446120714
4	1.6472830530136893	1.8150986802307134
5	1.142785770186043	1.5740076815094632
6	0.8557129857787168	1.4143320799983283
7	0.6952783138401766	1.3406911509149375
8	0.6108820991419437	1.3462304367188742
9	0.5421274770688139	1.3440516183750992
>10	7.422695764635572	47.743993757810806
>50	0.6178419943907753	10.590673834066301
>100	0.046646508742903436	2.2344138699017644
>500	0.004304776257519254	0.8033210835159639
>1k	0.002439373212594244	1.1021108325833835
>5k	0.0	0.0
>10k+	1.4349254191730847E-4	1.743842751634787
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44117	1.6892599489128766	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	3000	0.11487136130604143	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	2747	0.10518387650256526	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.658090753736095E-5	0.0	0.0	0.04594854452241657	0.0
2	7.658090753736095E-5	0.0	0.0	0.16529988891939362	0.0
3	7.658090753736095E-5	0.0	0.0	0.3211037453041545	0.0
4	7.658090753736095E-5	0.0	0.0	0.513015499592781	0.0
5	7.658090753736095E-5	0.0	0.0	0.9115808328709761	0.0
6	7.658090753736095E-5	0.0	0.0	1.588288022324866	0.0
7	7.658090753736095E-5	0.0	0.0	2.009789337410501	0.0
8	7.658090753736095E-5	0.0	0.0	2.8255674549522385	0.0
9	1.1487136130604143E-4	0.0	0.0	3.3177146572410887	0.0
10	1.1487136130604143E-4	0.0	0.0	4.050211037835946	0.0
11	1.1487136130604143E-4	0.0	0.0	4.433421899152901	0.0
12	1.1487136130604143E-4	0.0	0.0	4.805222205246788	0.0
13	1.1487136130604143E-4	0.0	0.0	4.989858773319365	0.0
14	1.1487136130604143E-4	0.0	0.0	5.093779064847563	0.0
15	1.1487136130604143E-4	0.0	0.0	5.184335988010493	0.0
16	1.1487136130604143E-4	0.0	0.0	5.313221655395871	0.0
17	1.1487136130604143E-4	0.0	0.0	5.449229347182225	0.0
18	1.1487136130604143E-4	0.0	0.0	5.671390559948109	0.0
19	1.1487136130604143E-4	0.0	0.0	5.775310851476307	0.0
20	1.1487136130604143E-4	0.0	0.0	5.885778810598951	0.0
21	1.1487136130604143E-4	0.0	0.0	6.006125706793913	0.0
22	1.1487136130604143E-4	0.0	0.0	6.140065714076758	0.0
23	1.1487136130604143E-4	0.0	0.0	6.290547197387672	0.0
24	1.1487136130604143E-4	0.0	0.0	6.4100517035997235	0.0
25	1.1487136130604143E-4	0.0	0.0	6.513703961951542	0.0
26	1.1487136130604143E-4	0.0	0.0	6.619232452538025	0.0
27	1.1487136130604143E-4	0.0	0.0	6.752138617569115	0.0
28	1.1487136130604143E-4	0.0	0.0	6.866588783883701	0.0
29	1.1487136130604143E-4	0.0	0.0	6.997963330764044	0.0
30	1.531618150747219E-4	0.0	0.0	7.173831384923593	0.0
31	1.531618150747219E-4	0.0	0.0	7.30712045449237	0.0
32	1.531618150747219E-4	0.0	0.0	7.441251914044058	0.0
33	1.531618150747219E-4	0.0	0.0	7.580590875308286	0.0
34	1.531618150747219E-4	0.0	0.0	7.723069653781546	0.0
35	1.531618150747219E-4	0.0	0.0	7.87703556838541	0.0
36	1.531618150747219E-4	0.0	0.0	8.013196421986837	0.0
37	1.531618150747219E-4	0.0	0.0	8.15881501766913	0.0
38	1.531618150747219E-4	0.0	0.0	8.330356250552818	0.0
39	1.531618150747219E-4	0.0	0.0	8.563238790373934	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	28065	0.0	43.019775	1
TATTACG	85	0.0	42.35294	1
CTATACG	50	1.0822987E-9	40.5	1
GGGCGAT	5610	0.0	38.26203	7
AGGGCGA	2595	0.0	37.890175	6
CACGACC	970	0.0	37.80928	27
GCGCGAC	1165	0.0	37.660946	9
TAGGGCG	1270	0.0	37.38189	5
TCAGACG	1195	0.0	37.280334	22
ACACGAC	985	0.0	37.233505	26
CGTTCGG	740	0.0	37.094597	45
CGACCCG	1215	0.0	37.037037	32
GGCGATT	1305	0.0	36.89655	8
GTTTTTT	32510	0.0	36.874805	2
CGCTGAA	1220	0.0	36.70082	37
CGCGACC	1215	0.0	36.666664	10
ACCCGCT	1205	0.0	36.59751	34
GTTGATC	1875	0.0	36.48	16
AACACGT	940	0.0	36.38298	41
TAGGGAC	2005	0.0	36.359104	5
>>END_MODULE