FastQCFastQC Report
Thu 26 May 2016
SRR1546600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546600_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2198952
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201370.9157544139208131No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26700.12142147714001943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA3850.042.66233431
CGTTTTT106800.041.9452251
CGTAAGG2750.040.0909082
TCACGAC4050.040.025
TAGTACG800.039.3751
CGACGGT4200.039.10714328
TACGGGA5650.038.628324
CACGACG4200.038.5714326
GTACGGG7300.038.5273973
AGGGCGA24100.038.1846476
CGTTCGG8000.037.687545
CTCACGA4300.037.6744224
TAGGGTA11350.037.6651955
ACATACG5500.037.63636417
CTTAACG601.5643309E-1037.4999961
ATCGCTA301.1402612E-437.49999642
TAGGGAC19550.037.4040955
GCGATAT2050.037.3170749
CATACGA5550.037.297318
GGCGATA9600.037.265628
Produced by FastQC (version 0.11.5)