FastQCFastQC Report
Thu 26 May 2016
SRR1546599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546599_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2393155
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218630.9135638936884574No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26270.10977141054382186No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCA207.034819E-445.035
CGTTTTT134900.041.614161
CGGTCTA2200.040.90909231
TAATGCG1050.040.7142871
TCACGAC2250.040.025
TATCGTG700.038.571431
GTACGAT356.2511244E-638.5714326
GTTTACG950.037.8947331
CTCACGA2450.037.6530624
ACGTTAG1500.037.51
AGGGCGA26100.037.1551746
TAGGGCG11250.037.05
GGCGATT14650.036.860078
GGGCGAT52150.036.759357
CGTTAGG3750.036.6000022
TGATTCG3700.036.4864915
CATACGA2350.036.3829818
GTACGGG6100.036.147543
TAGGGAC15950.036.1128545
CGACGGT2500.036.028
Produced by FastQC (version 0.11.5)