FastQCFastQC Report
Thu 26 May 2016
SRR1546598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546598_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences536882
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50530.9411751558070489No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7220.13448020235358982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5370.10002197875883341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAT600.045.0000048
GGGCGTA351.2103192E-745.0000047
CGCATCG253.8880185E-545.021
GCGTTAG207.029925E-445.01
CGTCGGG207.029925E-445.03
GCATCGC253.8880185E-545.022
CGTTTTT39550.043.748421
CGTATGG750.042.02
CGTTAGG650.041.5384642
CGATAAG556.002665E-1140.90909210
TAGTGCG501.0786607E-940.51
CGGGGCG451.9250365E-840.06
GCGTATG451.9250365E-840.01
CTCACGA403.4536242E-739.37524
ATCGTAG403.4536242E-739.3751
TTGCGAG403.4536242E-739.3751
AGGGCGA6350.039.3307086
GGCGATA2750.039.2727288
TAGGGTG3300.038.863645
CTATGCG356.2420695E-638.571431
Produced by FastQC (version 0.11.5)