##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546598_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	536882
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.414077953814804	31.0	31.0	33.0	30.0	34.0
2	31.775133083247344	31.0	31.0	34.0	30.0	34.0
3	31.813940493441763	31.0	31.0	34.0	30.0	34.0
4	35.612641139021235	37.0	35.0	37.0	33.0	37.0
5	35.56049187717227	37.0	35.0	37.0	33.0	37.0
6	35.62471455552617	37.0	35.0	37.0	33.0	37.0
7	36.02858728733688	37.0	35.0	37.0	35.0	37.0
8	34.71156231723172	37.0	35.0	37.0	33.0	37.0
9	37.02520293099787	39.0	37.0	39.0	34.0	39.0
10	36.79873789771309	39.0	37.0	39.0	32.0	39.0
11	36.794804072403245	39.0	37.0	39.0	32.0	39.0
12	36.47638214728749	39.0	35.0	39.0	32.0	39.0
13	36.37651662748984	39.0	35.0	39.0	32.0	39.0
14	37.50177320156012	40.0	36.0	41.0	32.0	41.0
15	37.6816302278713	40.0	36.0	41.0	33.0	41.0
16	37.728498999780214	40.0	35.0	41.0	33.0	41.0
17	37.6386114639716	40.0	35.0	41.0	33.0	41.0
18	37.52062464377647	39.0	36.0	41.0	33.0	41.0
19	37.38268371820996	39.0	36.0	41.0	32.0	41.0
20	37.18803014442652	39.0	35.0	41.0	32.0	41.0
21	37.10613691649189	39.0	35.0	41.0	32.0	41.0
22	37.02135478559534	39.0	35.0	41.0	32.0	41.0
23	36.95154987501909	38.0	35.0	41.0	32.0	41.0
24	36.81311908389553	38.0	35.0	41.0	32.0	41.0
25	36.736813676003294	38.0	35.0	41.0	31.0	41.0
26	36.61641664276321	38.0	35.0	41.0	31.0	41.0
27	36.50680223959827	38.0	35.0	40.0	31.0	41.0
28	36.5105535294534	38.0	35.0	40.0	31.0	41.0
29	36.451585264546026	38.0	35.0	40.0	31.0	41.0
30	36.386250610003685	38.0	35.0	40.0	31.0	41.0
31	36.22331909060092	38.0	35.0	40.0	30.0	41.0
32	36.02348188242482	38.0	35.0	41.0	30.0	41.0
33	35.75387701580608	38.0	35.0	41.0	29.0	41.0
34	35.50215876114304	38.0	35.0	41.0	27.0	41.0
35	35.29973066707396	38.0	35.0	41.0	25.0	41.0
36	35.14734336409118	38.0	35.0	41.0	24.0	41.0
37	35.0981817233582	38.0	35.0	41.0	24.0	41.0
38	35.01591411148073	38.0	34.0	41.0	24.0	41.0
39	34.948472848782416	38.0	34.0	40.0	23.0	41.0
40	34.82902201973618	38.0	34.0	40.0	23.0	41.0
41	34.754981541567794	38.0	34.0	40.0	23.0	41.0
42	34.696089643534336	38.0	34.0	40.0	23.0	41.0
43	34.61675563717912	38.0	34.0	40.0	23.0	41.0
44	34.50179741544697	38.0	34.0	40.0	23.0	41.0
45	34.41217809500039	37.0	34.0	40.0	23.0	41.0
46	34.34242161219784	37.0	34.0	40.0	22.0	41.0
47	34.28484285187434	37.0	34.0	40.0	22.0	41.0
48	34.21963671719298	37.0	34.0	40.0	22.0	41.0
49	34.15868663877723	37.0	34.0	40.0	22.0	41.0
50	34.06237124731319	37.0	34.0	40.0	21.0	41.0
51	33.70067910639582	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	3.0
15	17.0
16	46.0
17	127.0
18	267.0
19	535.0
20	973.0
21	1517.0
22	2282.0
23	3473.0
24	5318.0
25	8294.0
26	10830.0
27	11384.0
28	10703.0
29	10808.0
30	12383.0
31	14564.0
32	18141.0
33	23317.0
34	37835.0
35	59217.0
36	39453.0
37	50844.0
38	75132.0
39	139303.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.44958855018421	24.47036779031519	26.630805279372378	16.44923838012822
2	34.89574990407576	24.704683710759532	27.460410294999647	12.939156090165064
3	28.29802451935435	23.7992706032238	34.27922709273174	13.623477784690118
4	23.652683457445026	27.083046181470046	34.075271661184395	15.188998699900536
5	20.896025569864516	31.23498273363607	33.806497517145296	14.062494179354124
6	18.36381178732012	40.628667006902816	30.905301351134884	10.102219854642176
7	83.99871852660361	3.614202003419746	10.733829780100654	1.6532496898759876
8	81.84684902827809	5.740553790218335	10.013187255300048	2.399409926203523
9	79.3790814368893	4.876676811664389	12.680067500866112	3.0641742505802023
10	38.72508297912763	21.720042765449392	23.837454040180152	15.717420215242829
11	33.98921923253154	25.79654374704311	24.284293382903506	15.92994363752184
12	28.148457202886295	22.497681054682406	31.092493322555047	18.26136841987625
13	21.20950227424276	30.93081906266181	32.14952261390771	15.710156049187718
14	18.79556401592901	33.00948811843198	32.328891637268526	15.866056228370478
15	17.560097004555935	24.88107256343107	41.282628212530874	16.276202219482123
16	18.679337359047242	22.310675343930324	40.42825052804899	18.581736768973443
17	19.119657578387802	23.14102540222991	32.083958858743635	25.655358160638652
18	21.60158098055066	24.378541280951865	34.78082707187054	19.23905066662693
19	24.792971267429344	26.47024858348762	29.624945518754586	19.111834630328453
20	25.51081988220875	24.632787092880744	29.679333633833878	20.177059391076625
21	22.03556833717651	27.786180203471154	31.224179614887444	18.95407184446489
22	22.118268073803925	24.146087967188322	30.46665747780704	23.26898648120071
23	19.528872266159045	28.386125815356074	29.74061339363212	22.34438852485276
24	20.183392253791336	25.5048595408302	35.84418922593792	18.46755897944055
25	19.42139986067702	26.346757760550737	33.979906199127555	20.251936179644687
26	18.333637559091198	29.76613110515905	31.482709422182154	20.417521913567597
27	19.66614637853383	28.37271504725433	31.694487801788846	20.266650772422988
28	18.691816823808583	27.134640386528137	35.46887397975719	18.70466880990609
29	18.664995287605098	25.976471552408164	34.46623280348382	20.89230035650292
30	19.314858758535394	27.97188208954668	32.804973904880406	19.908285247037526
31	23.25259554240969	26.599886008471135	29.17829243669931	20.96922601241986
32	23.202863944032394	26.92044061823641	29.948107777872977	19.92858765985822
33	22.696421187523516	27.30134368445953	28.521164799713905	21.481070328303055
34	19.456975648280256	27.729184439038747	29.87267220730067	22.941167705380323
35	19.694271739413875	27.84038205788237	30.24035076609013	22.224995436613632
36	24.836556263760006	26.601748615151934	28.179562734455615	20.382132386632446
37	20.447509881128443	30.7358041431823	28.74225621272458	20.074429762964673
38	21.486285627009284	30.39848607328985	27.319224708595186	20.79600359110568
39	20.87665446038422	29.735770616262048	28.086246139747654	21.30132878360608
40	22.35239773358019	27.025677895701477	27.97728364892099	22.64464072179734
41	19.05726025458108	26.2623816779106	29.184997820750187	25.495360246758132
42	21.465424432184356	27.9413353399816	27.542737510290905	23.050502717543147
43	21.239303981135517	27.509955632708866	28.323914752217433	22.926825633938183
44	20.64475992862491	28.67240846219467	28.408477095525647	22.27435451365477
45	19.80230292690014	30.83098334457106	27.004257918872305	22.3624558096565
46	22.129443713888712	28.936153568195618	27.673306238614813	21.261096479300853
47	21.42966238391304	28.39897780145358	28.188689507191523	21.98267030744186
48	22.372700146400884	26.142802329003395	29.244414973867627	22.240082550728093
49	21.36633375676592	25.502624412813248	30.100469004362225	23.030572826058613
50	20.585156514839387	28.411643526883	29.112728681535234	21.890471276742378
51	19.859112430664467	29.339966696592544	27.390935065805895	23.40998580693709
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	698.0
1	808.0
2	918.0
3	4518.5
4	8119.0
5	5736.5
6	3354.0
7	3505.0
8	3656.0
9	3852.0
10	4048.0
11	4236.5
12	4425.0
13	4465.0
14	4505.0
15	4369.0
16	4233.0
17	4135.0
18	4037.0
19	3887.5
20	3738.0
21	3734.5
22	3731.0
23	3767.0
24	3803.0
25	4047.5
26	4615.0
27	4938.0
28	5459.5
29	5981.0
30	7285.5
31	8590.0
32	9421.0
33	10252.0
34	11917.0
35	13582.0
36	15136.5
37	16691.0
38	19387.5
39	22084.0
40	26636.5
41	31189.0
42	36180.0
43	41171.0
44	44938.0
45	48705.0
46	49515.0
47	50325.0
48	47886.5
49	45448.0
50	41839.0
51	38230.0
52	35732.0
53	33234.0
54	29754.5
55	26275.0
56	24325.0
57	22375.0
58	20133.0
59	17891.0
60	15764.5
61	13638.0
62	12103.0
63	10568.0
64	9328.0
65	8088.0
66	6674.0
67	5260.0
68	4178.5
69	3097.0
70	2581.5
71	2066.0
72	1824.5
73	1583.0
74	1289.5
75	775.5
76	555.0
77	396.0
78	237.0
79	195.5
80	154.0
81	114.0
82	74.0
83	46.0
84	18.0
85	15.0
86	12.0
87	9.5
88	7.0
89	5.5
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	536882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.490368707082816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25726972896094	28.581896910490062
2	7.433649753669871	5.722478397161375
3	3.686334990741892	4.256651789144284
4	2.4343722007326143	3.747995343058838
5	1.7673543251029469	3.401305980463497
6	1.4001380526157128	3.2335097931597425
7	1.1665745404542203	3.1431318930465046
8	1.00433972934942	3.0925925191864754
9	0.8368657028951	2.8990142516469977
>10	5.955239397951504	38.76558432467318
>50	0.04203618880084054	1.0377967987556052
>100	0.014341758531988042	0.916542844092097
>500	9.890867953095202E-4	0.23965263566187955
>1k	0.0	0.0
>5k	4.945433976547601E-4	0.9618465194594736
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5053	0.9411751558070489	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	722	0.13448020235358982	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	537	0.10002197875883341	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08176843328701651	0.0
2	0.0	0.0	0.0	0.2495892952268841	0.0
3	0.0	0.0	0.0	0.4071658204223647	0.0
4	0.0	0.0	0.0	0.6118662946420256	0.0
5	0.0	0.0	0.0	0.994818228214021	0.0
6	0.0	0.0	0.0	1.6700131500031665	0.0
7	0.0	0.0	0.0	2.0170167746357675	0.0
8	0.0	0.0	0.0	2.8821975778662723	0.0
9	0.0	0.0	0.0	3.328664399253467	0.0
10	0.0	0.0	0.0	4.001810453693735	0.0
11	0.0	0.0	0.0	4.440081805685421	0.0
12	0.0	0.0	0.0	4.848365190116264	0.0
13	0.0	0.0	0.0	5.042076284919219	0.0
14	0.0	0.0	0.0	5.117884376827683	0.0
15	0.0	0.0	0.0	5.208965843518688	0.0
16	0.0	0.0	0.0	5.402676938321642	0.0
17	0.0	0.0	0.0	5.634757730749029	0.0
18	0.0	0.0	0.0	6.003553853546962	0.0
19	0.0	0.0	0.0	6.1508860419980556	0.0
20	0.0	0.0	0.0	6.310883955878573	0.0
21	0.0	0.0	0.0	6.520240946800228	0.0
22	0.0	0.0	0.0	6.745057573172503	0.0
23	0.0	0.0	0.0	7.012155371198886	0.0
24	0.0	0.0	0.0	7.211454286044233	0.0
25	0.0	0.0	0.0	7.373314806605548	0.0
26	0.0	0.0	0.0	7.5375967158519	0.0
27	0.0	0.0	0.0	7.717897042553112	0.0
28	0.0	0.0	0.0	7.903040146624398	0.0
29	0.0	0.0	0.0	8.10699557817174	0.0
30	0.0	0.0	0.0	8.3439191479692	0.0
31	0.0	0.0	0.0	8.544521887491106	0.0
32	0.0	0.0	0.0	8.743820802336455	0.0
33	0.0	0.0	0.0	8.93734563647133	0.0
34	0.0	0.0	0.0	9.137575854657076	0.0
35	0.0	0.0	0.0	9.358294746331596	0.0
36	0.0	0.0	0.0	9.557221139840784	0.0
37	0.0	0.0	0.0	9.77402855748563	0.0
38	0.0	0.0	0.0	9.986738240432722	0.0
39	0.0	0.0	0.0	10.241729095033918	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	60	0.0	45.000004	8
GGGCGTA	35	1.2103192E-7	45.000004	7
CGCATCG	25	3.8880185E-5	45.0	21
GCGTTAG	20	7.029925E-4	45.0	1
CGTCGGG	20	7.029925E-4	45.0	3
GCATCGC	25	3.8880185E-5	45.0	22
CGTTTTT	3955	0.0	43.74842	1
CGTATGG	75	0.0	42.0	2
CGTTAGG	65	0.0	41.538464	2
CGATAAG	55	6.002665E-11	40.909092	10
TAGTGCG	50	1.0786607E-9	40.5	1
CGGGGCG	45	1.9250365E-8	40.0	6
GCGTATG	45	1.9250365E-8	40.0	1
CTCACGA	40	3.4536242E-7	39.375	24
ATCGTAG	40	3.4536242E-7	39.375	1
TTGCGAG	40	3.4536242E-7	39.375	1
AGGGCGA	635	0.0	39.330708	6
GGCGATA	275	0.0	39.272728	8
TAGGGTG	330	0.0	38.86364	5
CTATGCG	35	6.2420695E-6	38.57143	1
>>END_MODULE