FastQCFastQC Report
Thu 26 May 2016
SRR1546597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546597_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2107415
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122810.582751854760453No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43360.20574969808983992No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34650.1644194427770515No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT32590.15464443405783865TruSeq Adapter, Index 13 (95% over 22bp)
GAGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT28160.13362342016166726No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC28020.13295909918074988TruSeq Adapter, Index 19 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT23160.10989767084318941No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC22580.10714548392224596TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA207.034625E-445.00000416
ATCCGAT207.034625E-445.00000419
TACGATA207.034625E-445.00000427
CGCTATA207.034625E-445.00000430
CCGAAAC207.034625E-445.00000440
TCACGAC1500.045.025
AATCCCG253.891917E-545.036
CATCTCG253.891917E-545.034
GTCGACG302.1663673E-644.9999961
ACGTATC302.1663673E-644.99999634
CTCCGAT800.042.18750438
TGCGAAG2300.041.086961
CGTTTTT81400.040.853811
ACGTTAG501.0822987E-940.51
TAGGGCG8600.040.29075
ACGTAGG2850.040.2631572
CGTAAGG3650.040.0684932
TCCGATT850.039.70588339
ATTCGGC2850.039.47368617
AGGGCGA21750.038.689666