FastQCFastQC Report
Thu 26 May 2016
SRR1546596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546596_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1026168
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61160.5960037732612984No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG27170.26477146042363436No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC23670.23066398484458683RNA PCR Primer, Index 34 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18460.17989257119691904No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC16930.16498273187236398No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11160.10875412213204856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG302.1652722E-645.0000041
CGAACCC406.8139343E-945.00000434
CGGCATA207.0329313E-445.00000414
TAACGCC600.045.00000412
TAACACG207.0329313E-445.0000041
ACGCTAG207.0329313E-445.0000041
TACGTAT207.0329313E-445.00000445
TACGTAG600.045.0000041
CAAACGA253.8905127E-545.015
CGTTCGA253.8905127E-545.014
TACGACG351.2117562E-745.01
CGGTCTA750.045.031
TTCGCTA253.8905127E-545.01
AATGTCG253.8905127E-545.01
ATAGTAG1100.042.9545441
GCGATAA1500.042.09
TGATTCG1450.041.89655315
CGTTCGG1400.041.78571345
TCGATAG700.041.7857131
GTGTAAG1850.041.3513531