##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546596_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1026168
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.397432973937992	31.0	31.0	33.0	30.0	34.0
2	31.752874772941663	31.0	31.0	34.0	30.0	34.0
3	31.84812330924371	31.0	31.0	34.0	30.0	34.0
4	35.61722057207007	37.0	35.0	37.0	33.0	37.0
5	35.51971412088469	37.0	35.0	37.0	33.0	37.0
6	35.58592160348013	37.0	35.0	37.0	33.0	37.0
7	35.93168662441238	37.0	35.0	37.0	35.0	37.0
8	34.58796610301627	37.0	35.0	37.0	33.0	37.0
9	36.85442539623141	39.0	37.0	39.0	33.0	39.0
10	36.817488949177914	39.0	37.0	39.0	32.0	39.0
11	36.72214003944773	39.0	35.0	39.0	32.0	39.0
12	36.15742159178614	38.0	35.0	39.0	32.0	39.0
13	35.863046791558496	38.0	35.0	39.0	31.0	39.0
14	36.92544398188211	39.0	35.0	41.0	31.0	41.0
15	37.165207841211185	39.0	35.0	41.0	32.0	41.0
16	37.30069150470488	39.0	35.0	41.0	32.0	41.0
17	37.17111720498008	39.0	35.0	41.0	32.0	41.0
18	37.144457827568196	39.0	35.0	41.0	32.0	41.0
19	37.06444266435905	38.0	35.0	41.0	32.0	41.0
20	36.82519821315808	38.0	35.0	41.0	32.0	41.0
21	36.756901404058596	38.0	35.0	41.0	31.0	41.0
22	36.65010797452269	38.0	35.0	41.0	31.0	41.0
23	36.54905239687849	38.0	35.0	41.0	31.0	41.0
24	36.441289340536834	38.0	35.0	40.0	31.0	41.0
25	36.37424768653866	38.0	35.0	40.0	31.0	41.0
26	36.21645773401626	38.0	35.0	40.0	31.0	41.0
27	36.064024604158384	38.0	35.0	40.0	30.0	41.0
28	36.13037826164916	38.0	35.0	40.0	30.0	41.0
29	36.091663353369036	38.0	35.0	40.0	30.0	41.0
30	36.0538079534735	37.0	35.0	40.0	30.0	41.0
31	35.93093041295383	37.0	35.0	40.0	30.0	41.0
32	35.730327782585306	38.0	35.0	40.0	30.0	41.0
33	35.561276516126014	38.0	35.0	40.0	29.0	41.0
34	35.38514843573372	37.0	35.0	40.0	28.0	41.0
35	35.241058968901775	37.0	34.0	40.0	27.0	41.0
36	35.08197000880947	37.0	34.0	40.0	26.0	41.0
37	34.9764824083386	37.0	34.0	40.0	26.0	41.0
38	34.95061724785805	37.0	34.0	40.0	26.0	41.0
39	34.89302044109736	37.0	34.0	40.0	25.0	41.0
40	34.768643146151504	37.0	34.0	40.0	24.0	41.0
41	34.72508595083846	37.0	34.0	40.0	24.0	41.0
42	34.62119945272119	36.0	34.0	40.0	24.0	41.0
43	34.52022378401977	36.0	34.0	40.0	24.0	41.0
44	34.4378230465187	36.0	34.0	40.0	23.0	41.0
45	34.326805162507505	36.0	34.0	40.0	23.0	41.0
46	34.265422425957546	36.0	34.0	40.0	23.0	41.0
47	34.14832756429746	35.0	33.0	40.0	23.0	41.0
48	34.12106497181748	35.0	34.0	40.0	23.0	41.0
49	34.105901762674335	36.0	34.0	40.0	24.0	41.0
50	33.9400254149418	35.0	33.0	40.0	23.0	41.0
51	33.59180660476647	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	4.0
13	5.0
14	16.0
15	33.0
16	89.0
17	211.0
18	544.0
19	1048.0
20	2032.0
21	3380.0
22	5258.0
23	7137.0
24	10002.0
25	12970.0
26	16568.0
27	18656.0
28	20176.0
29	21998.0
30	25915.0
31	31153.0
32	38915.0
33	50098.0
34	86141.0
35	146596.0
36	74763.0
37	92123.0
38	133360.0
39	226730.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.04689290642468	24.04167738615899	26.78333372313305	14.128095984283275
2	31.90325560726899	27.184340186012427	28.207272103593173	12.705132103125413
3	28.475454311574712	27.090495903204932	30.925735357173483	13.50831442804687
4	25.781645890341544	27.01701865581464	32.41048249409453	14.790852959749282
5	24.172942442173213	31.073664351256326	29.508521021898947	15.244872184671515
6	20.485729432217724	41.90736799432452	27.024522300442033	10.582380273015724
7	83.39784518714285	4.985538430354484	9.556427407597976	2.060188974904694
8	80.82185373155272	8.427177616140828	7.479477044694435	3.2714916076120084
9	78.23855353119568	6.139345604228548	10.24081826757412	5.38128259700166
10	47.21234729595933	22.764011350967873	16.287781337948562	13.73586001512423
11	41.67124681338728	22.92421903625917	21.38636168736503	14.018172462988515
12	36.052283836564776	21.335687723647588	25.676302515767397	16.93572592402024
13	23.00217898043985	34.62873525582555	26.86402226536006	15.505063498374536
14	16.78565303147243	39.96996593150439	27.852846707361756	15.391534329661418
15	15.751124572194804	23.465163598942862	44.760312151616496	16.02339967724583
16	17.180325248887122	19.642105386252542	42.175550202306056	21.00201916255428
17	17.15810666479563	21.218650357446343	29.55022959203561	32.07301338572242
18	21.59909488504806	23.55023738803003	33.64488076026538	21.205786966656532
19	26.93301681595996	24.566445260425194	26.63462512960841	21.86591279400644
20	30.242318996499602	23.127986840361423	25.464933617107533	21.16476054603145
21	23.284101628583233	27.87087494445354	27.727136297370414	21.117887129592816
22	22.165668779381154	24.255287633214053	27.089229054111996	26.489814533292794
23	21.370964598389346	28.86086878561795	25.04745811601999	24.720708499972712
24	21.034469989319486	24.79291889827007	34.894188865760775	19.278422246649672
25	19.37129203015491	26.274742537284347	32.564258483990926	21.789706948569826
26	17.66523610169095	31.197620662503606	28.67415471930522	22.462988516500225
27	20.308273109276453	30.724111451536203	28.189341316431616	20.77827412275573
28	17.386919100965923	29.001391585003628	34.920792696712425	18.690896617318025
29	19.038403068503403	24.601429785376276	34.28541915163989	22.074747994480436
30	20.197862338330566	28.803958026366054	30.736487592674884	20.2616920426285
31	27.51186940150151	27.023352901279324	24.346305868044997	21.118471829174172
32	28.921482642218425	26.800387460922575	25.492804297152123	18.78532559970687
33	26.471883746131237	26.960595146213873	25.10758472296934	21.459936384685548
34	21.250126684909294	27.8748703915928	27.094393900413966	23.78060902308394
35	22.621247203187004	25.776383594109348	29.097867015927214	22.504502186776435
36	28.52203537822267	26.24005036212394	25.045996367066603	20.19191789258679
37	21.55875061393456	30.399700633814348	28.194798512524265	19.84675023972683
38	21.989089505811915	30.453005745647886	24.07383586313352	23.48406888540668
39	21.43547645219886	29.796875365437238	25.620950955399113	23.146697226964786
40	22.499824590125595	26.703424780347856	26.470714346968528	24.32603628255802
41	18.33237832401712	25.485885352106084	27.489163567758883	28.692572756117908
42	23.042328351692902	27.16416805045568	25.376741430253137	24.41676216759829
43	22.837391148427937	25.61734530798076	26.27756858526089	25.26769495833041
44	21.64908669925392	28.06616460462614	27.779369459971466	22.505379236148467
45	19.50372648533184	32.04874835309618	24.87770033756656	23.569824824005426
46	23.388470503855117	29.7639372890209	26.624197987074243	20.22339422004974
47	22.803186222918665	27.843783863850753	27.412373022740915	21.940656890489667
48	23.180707252613608	25.014714939464106	28.769558200996325	23.03501960692596
49	22.045902815133584	23.787138168409072	30.57384365912794	23.593115357329403
50	21.036029188203102	29.052552798372194	27.4160761200895	22.495341893335205
51	20.261009893116917	30.036212394071924	25.46844181459566	24.234335898215498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1481.0
1	1952.0
2	2423.0
3	6541.0
4	10659.0
5	7638.5
6	4618.0
7	4756.5
8	4895.0
9	5268.0
10	5641.0
11	5886.5
12	6132.0
13	6016.0
14	5900.0
15	5723.5
16	5547.0
17	5419.0
18	5291.0
19	4870.0
20	4449.0
21	4471.0
22	4493.0
23	4766.5
24	5040.0
25	5558.0
26	7094.0
27	8112.0
28	9330.0
29	10548.0
30	11644.5
31	12741.0
32	16024.5
33	19308.0
34	20141.0
35	20974.0
36	23152.0
37	25330.0
38	30435.0
39	35540.0
40	46412.0
41	57284.0
42	68360.5
43	79437.0
44	82099.5
45	84762.0
46	83569.5
47	82377.0
48	87609.0
49	92841.0
50	89905.0
51	86969.0
52	78768.5
53	70568.0
54	64185.0
55	57802.0
56	53599.5
57	49397.0
58	44430.0
59	39463.0
60	37106.5
61	34750.0
62	30028.0
63	25306.0
64	22512.0
65	19718.0
66	16701.5
67	13685.0
68	11197.0
69	8709.0
70	7764.5
71	6820.0
72	5917.0
73	5014.0
74	3775.5
75	2112.5
76	1688.0
77	1414.0
78	1140.0
79	752.5
80	365.0
81	314.5
82	264.0
83	151.0
84	38.0
85	25.0
86	12.0
87	12.5
88	13.0
89	9.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1026168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.833064890164817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16316957922815	20.155302568524238
2	6.450618562675609	3.2037725869725016
3	2.0409919692274827	1.5205225803639404
4	1.179426917688464	1.1715514072065882
5	0.7782142604629062	0.9662722614263488
6	0.5665191580474807	0.8441044207988774
7	0.43535909472613815	0.7567910454900321
8	0.32820008332359424	0.6520171172905852
9	0.2972202237187054	0.6642800192048324
>10	4.781899564503224	30.936613737647328
>50	1.7836930726650335	30.3001511218786
>100	0.1882248576757094	6.594935250590943
>500	0.004039160035959429	0.6433544950841885
>1k	0.0020195800179797145	0.9768676999727022
>5k	4.039160035959429E-4	0.6134636875483157
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6116	0.5960037732612984	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	2717	0.26477146042363436	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	2367	0.23066398484458683	RNA PCR Primer, Index 34 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1846	0.17989257119691904	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	1693	0.16498273187236398	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1116	0.10875412213204856	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.744993022584996E-5	0.0	0.0	0.07065119941374122	0.0
2	9.744993022584996E-5	0.0	0.0	0.263017361679569	0.0
3	9.744993022584996E-5	0.0	0.0	0.38395272508984885	0.0
4	9.744993022584996E-5	0.0	0.0	0.651452783559807	0.0
5	9.744993022584996E-5	0.0	0.0	1.2411223113564251	0.0
6	9.744993022584996E-5	0.0	0.0	1.7729065805988884	0.0
7	9.744993022584996E-5	0.0	0.0	2.0982918976230014	0.0
8	9.744993022584996E-5	0.0	0.0	2.9112192155670416	0.0
9	9.744993022584996E-5	0.0	0.0	3.2715890575422346	0.0
10	9.744993022584996E-5	0.0	0.0	4.157116573504533	0.0
11	9.744993022584996E-5	0.0	0.0	4.662589361586017	0.0
12	9.744993022584996E-5	0.0	0.0	5.294162359379751	0.0
13	9.744993022584996E-5	0.0	0.0	5.504556758737361	0.0
14	9.744993022584996E-5	0.0	0.0	5.5921642460104	0.0
15	9.744993022584996E-5	0.0	0.0	5.737169742186465	0.0
16	9.744993022584996E-5	0.0	0.0	5.975434821588668	0.0
17	9.744993022584996E-5	0.0	0.0	6.218962197223067	0.0
18	9.744993022584996E-5	0.0	0.0	6.6076899688939825	0.0
19	9.744993022584996E-5	0.0	0.0	6.803661778578166	0.0
20	9.744993022584996E-5	0.0	0.0	6.971275658566628	0.0
21	9.744993022584996E-5	0.0	0.0	7.154968777042356	0.0
22	9.744993022584996E-5	0.0	0.0	7.350843136796314	0.0
23	1.9489986045169991E-4	0.0	0.0	7.556949739223986	0.0
24	1.9489986045169991E-4	0.0	0.0	7.716572724933929	0.0
25	1.9489986045169991E-4	0.0	0.0	7.834097340786304	0.0
26	1.9489986045169991E-4	0.0	0.0	7.961561849521716	0.0
27	2.9234979067754987E-4	0.0	0.0	8.107346945139588	0.0
28	2.9234979067754987E-4	0.0	0.0	8.233447154851838	0.0
29	2.9234979067754987E-4	0.0	0.0	8.374554653818867	0.0
30	2.9234979067754987E-4	0.0	0.0	8.56916216447989	0.0
31	2.9234979067754987E-4	0.0	0.0	8.728297900538703	0.0
32	2.9234979067754987E-4	0.0	0.0	8.87515494538906	0.0
33	2.9234979067754987E-4	0.0	0.0	9.02425333863461	0.0
34	2.9234979067754987E-4	0.0	0.0	9.17851657818213	0.0
35	2.9234979067754987E-4	0.0	0.0	9.365230644494858	0.0
36	2.9234979067754987E-4	0.0	0.0	9.52085818306554	0.0
37	2.9234979067754987E-4	0.0	0.0	9.676875521357127	0.0
38	2.9234979067754987E-4	0.0	0.0	9.827728013346743	0.0
39	2.9234979067754987E-4	0.0	0.0	9.985012200731264	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.1652722E-6	45.000004	1
CGAACCC	40	6.8139343E-9	45.000004	34
CGGCATA	20	7.0329313E-4	45.000004	14
TAACGCC	60	0.0	45.000004	12
TAACACG	20	7.0329313E-4	45.000004	1
ACGCTAG	20	7.0329313E-4	45.000004	1
TACGTAT	20	7.0329313E-4	45.000004	45
TACGTAG	60	0.0	45.000004	1
CAAACGA	25	3.8905127E-5	45.0	15
CGTTCGA	25	3.8905127E-5	45.0	14
TACGACG	35	1.2117562E-7	45.0	1
CGGTCTA	75	0.0	45.0	31
TTCGCTA	25	3.8905127E-5	45.0	1
AATGTCG	25	3.8905127E-5	45.0	1
ATAGTAG	110	0.0	42.954544	1
GCGATAA	150	0.0	42.0	9
TGATTCG	145	0.0	41.896553	15
CGTTCGG	140	0.0	41.785713	45
TCGATAG	70	0.0	41.785713	1
GTGTAAG	185	0.0	41.351353	1
>>END_MODULE