FastQCFastQC Report
Thu 26 May 2016
SRR1546595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546595_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3434931
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286630.8344563544362318No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36880.10736751335034096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA2700.041.66666831
CGTTTTT174350.041.154291
TATCACG501.0822987E-940.5000041
TCGATAG1950.039.230771
TCACGAC2700.039.16666825
CGACGGT2750.038.4545428
TACGGGA9750.038.0769234
GCGATCG2700.037.5000049
CCGCGTA301.1403946E-437.50000414
TAGGGCG16350.037.431195
AGGGCGA38250.037.352946
ACGGGAT9950.037.3115585
CGTTCGA2500.036.89999814
CACGACC5750.036.78260827
TAGGGAC23100.036.720785
GGGCGAT80000.036.7031257
ACGGGTA2900.036.4655155
CGCATCG1550.036.2903221
GTAGGGC16150.036.083594
GCCAGTA20000.036.025
Produced by FastQC (version 0.11.5)