##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546595_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3434931
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.482555253657207	31.0	31.0	33.0	30.0	34.0
2	31.8500028093723	31.0	31.0	34.0	30.0	34.0
3	31.93461469822829	33.0	31.0	34.0	30.0	34.0
4	35.69713452759313	37.0	35.0	37.0	33.0	37.0
5	35.64075959604429	37.0	35.0	37.0	33.0	37.0
6	35.68952243873312	37.0	35.0	37.0	33.0	37.0
7	36.037189393324056	37.0	35.0	37.0	35.0	37.0
8	34.70381413775124	37.0	35.0	37.0	33.0	37.0
9	36.97688716309003	39.0	37.0	39.0	33.0	39.0
10	36.94218486484881	39.0	37.0	39.0	32.0	39.0
11	36.89864745463591	39.0	37.0	39.0	33.0	39.0
12	36.64098638371484	39.0	35.0	39.0	32.0	39.0
13	36.576270382141594	39.0	35.0	39.0	32.0	39.0
14	37.701235920022846	40.0	36.0	41.0	33.0	41.0
15	37.83850039491332	40.0	36.0	41.0	33.0	41.0
16	37.87877078171294	40.0	36.0	41.0	33.0	41.0
17	37.780249443147476	40.0	36.0	41.0	33.0	41.0
18	37.68701001563059	39.0	36.0	41.0	33.0	41.0
19	37.60774554132237	39.0	36.0	41.0	33.0	41.0
20	37.45208477259077	39.0	35.0	41.0	33.0	41.0
21	37.35332645692155	39.0	35.0	41.0	32.0	41.0
22	37.265988166865654	39.0	35.0	41.0	32.0	41.0
23	37.19406765376073	39.0	35.0	41.0	32.0	41.0
24	37.06500858386966	39.0	35.0	41.0	32.0	41.0
25	36.96192645500012	39.0	35.0	41.0	32.0	41.0
26	36.86491053240953	39.0	35.0	41.0	31.0	41.0
27	36.73444706749568	38.0	35.0	41.0	31.0	41.0
28	36.73507298982134	38.0	35.0	41.0	31.0	41.0
29	36.69352572147737	38.0	35.0	41.0	31.0	41.0
30	36.62306520858789	38.0	35.0	41.0	31.0	41.0
31	36.52253451379373	38.0	35.0	41.0	31.0	41.0
32	36.40489488726266	39.0	35.0	41.0	30.0	41.0
33	36.227950430445325	39.0	35.0	41.0	30.0	41.0
34	36.0395486838018	39.0	35.0	41.0	30.0	41.0
35	35.89797961007077	38.0	35.0	41.0	29.0	41.0
36	35.780109993475854	38.0	35.0	41.0	28.0	41.0
37	35.73747594929854	38.0	35.0	41.0	28.0	41.0
38	35.631151833908746	38.0	35.0	41.0	28.0	41.0
39	35.54523045732214	38.0	35.0	41.0	27.0	41.0
40	35.41865265998065	38.0	35.0	40.0	27.0	41.0
41	35.325743952353044	38.0	35.0	40.0	26.0	41.0
42	35.25363537142376	38.0	34.0	40.0	26.0	41.0
43	35.16467812599438	38.0	34.0	40.0	26.0	41.0
44	35.04346375516713	38.0	34.0	40.0	26.0	41.0
45	34.93190518237484	37.0	34.0	40.0	25.0	41.0
46	34.87212086647447	37.0	34.0	40.0	25.0	41.0
47	34.81528013226467	37.0	34.0	40.0	25.0	41.0
48	34.731262432928055	37.0	34.0	40.0	24.0	41.0
49	34.66412105512454	37.0	34.0	40.0	24.0	41.0
50	34.56030354030401	37.0	34.0	40.0	24.0	41.0
51	34.20166198389429	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	6.0
12	11.0
13	20.0
14	41.0
15	80.0
16	225.0
17	593.0
18	1290.0
19	2650.0
20	4634.0
21	7470.0
22	11472.0
23	16984.0
24	25264.0
25	37029.0
26	50583.0
27	58766.0
28	61648.0
29	67315.0
30	76403.0
31	92904.0
32	115293.0
33	149024.0
34	238906.0
35	356814.0
36	274894.0
37	355311.0
38	522229.0
39	906381.0
40	684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.31268342799317	23.623676865707054	26.59718637725183	15.46645332904795
2	34.27448760979478	24.85118332799116	27.443695375540294	13.430633686673765
3	29.89425406216311	24.38922353898812	31.75787228331515	13.958650115533617
4	25.831290351975046	27.32546883765642	30.942950527972762	15.900290282395774
5	22.885991014084418	31.54508780525722	30.065290976732868	15.503630203925495
6	20.85287884967704	40.25303565049778	28.26388652348475	10.630198976340427
7	84.4838513495613	4.235863835401642	9.103268741060592	2.1770160739764495
8	81.14940300110833	6.68301634006622	8.894705599617577	3.2728750592078852
9	77.82194169256967	5.869812232036102	12.110083142863715	4.198162932530523
10	39.401752174934515	27.086482959919717	18.410675498285116	15.101089366860645
11	32.086961863280514	24.896569974768052	26.263933686004172	16.75253447594726
12	28.37352482480725	22.520423263232946	30.870925791522453	18.235126120437354
13	24.077892685471703	26.529237414084882	31.462582508935405	17.93028739150801
14	19.86956943239908	29.891109894201662	31.959914187504783	18.279406485894476
15	18.803114240140488	23.94411998377842	39.0545836291908	18.19818214689029
16	21.89537431756271	21.79840002608495	36.53753161271653	19.76869404363581
17	21.663171691076183	22.106470260974675	30.94073796533322	25.289620082615926
18	23.15071248883893	22.80104025379258	33.34809345515237	20.700153802216114
19	26.55011119582897	24.803846132571515	28.482668210802487	20.16337446079703
20	27.418949609177012	23.936987380532535	27.830166020802167	20.81389698948829
21	25.060823638087637	26.18655221895287	29.53293676059286	19.219687382366633
22	24.04368530255775	23.173187467230054	28.97819490406066	23.80493232615153
23	21.89878049952095	26.710784001192454	28.282664193254536	23.107771306032056
24	21.82649374907385	24.59257551316169	32.75736834306133	20.823562394703124
25	22.120793692799072	24.975203286470673	30.970025307640825	21.933977713089433
26	20.294381459190884	27.67531574870063	29.674569882189772	22.355732909918714
27	20.669148812596237	26.349233798291728	30.44002921747191	22.54158817164013
28	20.20346260230555	26.290076860350325	33.045729302859364	20.46073123448477
29	21.078560238910185	24.773627184941997	31.773796911786583	22.374015664361234
30	21.895985683555217	26.265738671315376	31.041205776768148	20.797069868361255
31	25.02932373314049	25.750153350969786	27.766059929588106	21.454462986301618
32	25.38985499272038	25.763807191469056	28.448140588559127	20.398197227251437
33	24.474610989274602	26.72251640571528	27.161156949004216	21.6417156560059
34	21.174719375731275	26.74117762481983	29.474769653305994	22.609333346142908
35	21.351404147565116	26.76729168649967	29.837717264189585	22.043586901745623
36	24.762477033745363	26.90840078010301	27.202496935164056	21.126625250987573
37	21.837702125603105	28.760635948727938	27.733220841990715	21.668441083678243
38	22.686161672534325	29.242392350821607	26.49278835586508	21.57865762077899
39	22.006148012871293	27.255249086517313	27.35137911067209	23.387223789939306
40	23.756110384750087	25.007110768746156	28.332912655305158	22.9038661911986
41	20.751100968258168	25.076544477894895	29.160673096490147	25.011681457356786
42	22.8928033780009	25.988789876710772	27.399152996086386	23.71925374920195
43	21.81569294987294	26.004306927853865	28.772106339253977	23.407893783019222
44	21.92626285651735	26.847235068186233	28.740926673636242	22.485575401660178
45	21.007205093785	28.117013121952084	28.110724786029177	22.765056998233735
46	22.81396627763411	27.15073461446533	28.291776457809487	21.74352265009108
47	21.99863694496338	26.579485876135507	28.780811026480592	22.64106615242053
48	21.97217353128782	25.88069454670269	29.68522511805914	22.461906803950356
49	22.218524913600884	24.572953576068922	29.88784927557497	23.320672234755225
50	21.484827497262682	26.802750914064937	29.036070884684438	22.67635070398794
51	21.172535925758044	27.445907938179836	28.03410024830193	23.34745588776019
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3622.0
1	5022.5
2	6423.0
3	27886.0
4	49349.0
5	34033.0
6	18717.0
7	18748.0
8	18779.0
9	19706.0
10	20633.0
11	20690.0
12	20747.0
13	20765.0
14	20783.0
15	20045.5
16	19308.0
17	18493.0
18	17678.0
19	17131.0
20	16584.0
21	17485.5
22	18387.0
23	19222.0
24	20057.0
25	21947.0
26	28013.0
27	32189.0
28	36658.0
29	41127.0
30	47522.0
31	53917.0
32	61918.0
33	69919.0
34	77789.5
35	85660.0
36	94878.0
37	104096.0
38	112321.0
39	120546.0
40	144082.5
41	167619.0
42	192715.0
43	217811.0
44	241182.5
45	264554.0
46	278095.0
47	291636.0
48	279985.5
49	268335.0
50	252731.5
51	237128.0
52	222082.0
53	207036.0
54	193546.5
55	180057.0
56	173794.5
57	167532.0
58	157082.0
59	146632.0
60	136068.0
61	125504.0
62	115740.0
63	105976.0
64	97561.0
65	89146.0
66	75880.5
67	62615.0
68	53282.5
69	43950.0
70	36242.5
71	28535.0
72	24321.0
73	20107.0
74	16786.5
75	10467.0
76	7468.0
77	5654.0
78	3840.0
79	2921.5
80	2003.0
81	1493.0
82	983.0
83	667.0
84	351.0
85	231.0
86	111.0
87	96.5
88	82.0
89	82.0
90	82.0
91	42.0
92	2.0
93	4.5
94	7.0
95	6.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3434931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.289586995400278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97349612995039	17.82576199314837
2	7.008384460004646	3.1242799023697
3	2.3682781912014295	1.5836382831628044
4	1.2934564359225125	1.153224390130209
5	0.8346394440707486	0.9301884249203738
6	0.5930677068504546	0.7931540547603456
7	0.46598495809580764	0.727062858341712
8	0.36799240279826556	0.6561918940654657
9	0.3108660802462859	0.6236168885611806
>10	4.333180140894926	23.812400815860265
>50	1.8640441646322978	29.629229109126154
>100	0.5789942650387445	16.687801952569124
>500	0.005439728781102342	0.8340739890287142
>1k	0.0020398982929133785	0.7505332158871553
>5k	0.0	0.0
>10k+	1.3599321952755854E-4	0.868842228068398
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28663	0.8344563544362318	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3688	0.10736751335034096	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9112666309745377E-5	0.0	0.0	0.061806190575589434	0.0
2	2.9112666309745377E-5	0.0	0.0	0.19784968024102959	0.0
3	2.9112666309745377E-5	0.0	0.0	0.32416953935901477	0.0
4	2.9112666309745377E-5	0.0	0.0	0.5079578017724373	0.0
5	2.9112666309745377E-5	0.0	0.0	0.8400750990340127	0.0
6	2.9112666309745377E-5	0.0	0.0	1.4600002154337306	0.0
7	2.9112666309745377E-5	0.0	0.0	1.8115065484575963	0.0
8	2.9112666309745377E-5	0.0	0.0	2.69953020890376	0.0
9	2.9112666309745377E-5	0.0	0.0	3.1948240008314577	0.0
10	2.9112666309745377E-5	0.0	0.0	3.9334705704423176	0.0
11	2.9112666309745377E-5	0.0	0.0	4.406638735974609	0.0
12	2.9112666309745377E-5	0.0	0.0	4.836370803372761	0.0
13	2.9112666309745377E-5	0.0	0.0	5.034948300271534	0.0
14	2.9112666309745377E-5	0.0	0.0	5.120306637891708	0.0
15	2.9112666309745377E-5	2.9112666309745377E-5	0.0	5.218503661354479	0.0
16	2.9112666309745377E-5	2.9112666309745377E-5	0.0	5.407066401042699	0.0
17	2.9112666309745377E-5	2.9112666309745377E-5	0.0	5.6156004298194055	0.0
18	2.9112666309745377E-5	2.9112666309745377E-5	0.0	5.9311235072844255	0.0
19	2.9112666309745377E-5	2.9112666309745377E-5	0.0	6.0661771662953345	0.0
20	2.9112666309745377E-5	2.9112666309745377E-5	0.0	6.22449184568773	0.0
21	2.9112666309745377E-5	2.9112666309745377E-5	0.0	6.405630855466966	0.0
22	2.9112666309745377E-5	2.9112666309745377E-5	0.0	6.601646437730482	0.0
23	2.9112666309745377E-5	2.9112666309745377E-5	0.0	6.816527027762712	0.0
24	2.9112666309745377E-5	2.9112666309745377E-5	0.0	6.985758957021262	0.0
25	2.9112666309745377E-5	2.9112666309745377E-5	0.0	7.130594471912245	0.0
26	2.9112666309745377E-5	2.9112666309745377E-5	0.0	7.272373156840705	0.0
27	2.9112666309745377E-5	2.9112666309745377E-5	0.0	7.418489629049317	0.0
28	2.9112666309745377E-5	2.9112666309745377E-5	0.0	7.5674591425562845	0.0
29	2.9112666309745377E-5	2.9112666309745377E-5	0.0	7.728073722587149	0.0
30	2.9112666309745377E-5	2.9112666309745377E-5	0.0	7.949475549872763	0.0
31	2.9112666309745377E-5	2.9112666309745377E-5	0.0	8.123773083069208	0.0
32	2.9112666309745377E-5	2.9112666309745377E-5	0.0	8.29748836293946	0.0
33	2.9112666309745377E-5	2.9112666309745377E-5	0.0	8.472397262128409	0.0
34	2.9112666309745377E-5	2.9112666309745377E-5	0.0	8.649285822626423	0.0
35	2.9112666309745377E-5	2.9112666309745377E-5	0.0	8.84611655954661	0.0
36	2.9112666309745377E-5	2.9112666309745377E-5	0.0	9.02559614734619	0.0
37	2.9112666309745377E-5	2.9112666309745377E-5	0.0	9.211189395070818	0.0
38	2.9112666309745377E-5	2.9112666309745377E-5	0.0	9.408544159984581	0.0
39	2.9112666309745377E-5	2.9112666309745377E-5	0.0	9.639320265821933	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	270	0.0	41.666668	31
CGTTTTT	17435	0.0	41.15429	1
TATCACG	50	1.0822987E-9	40.500004	1
TCGATAG	195	0.0	39.23077	1
TCACGAC	270	0.0	39.166668	25
CGACGGT	275	0.0	38.45454	28
TACGGGA	975	0.0	38.076923	4
GCGATCG	270	0.0	37.500004	9
CCGCGTA	30	1.1403946E-4	37.500004	14
TAGGGCG	1635	0.0	37.43119	5
AGGGCGA	3825	0.0	37.35294	6
ACGGGAT	995	0.0	37.311558	5
CGTTCGA	250	0.0	36.899998	14
CACGACC	575	0.0	36.782608	27
TAGGGAC	2310	0.0	36.72078	5
GGGCGAT	8000	0.0	36.703125	7
ACGGGTA	290	0.0	36.465515	5
CGCATCG	155	0.0	36.29032	21
GTAGGGC	1615	0.0	36.08359	4
GCCAGTA	2000	0.0	36.0	25
>>END_MODULE