FastQCFastQC Report
Thu 26 May 2016
SRR1546594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546594_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1251819
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66080.5278718408971265No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29050.2320623029367664No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG17450.13939714926838465No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC15100.1206244672752211No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14530.1160710933449644No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT12890.10297015782633112Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC12520.1000144589593224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGT351.2120472E-745.00000413
CGAATAT650.045.00000414
CGTATTA207.033528E-445.014
ATTAACG406.8157533E-945.01
ATATCGA207.033528E-445.020
CTTGCGA207.033528E-445.024
CGCTAAA207.033528E-445.030
TTGCGTT207.033528E-445.011
ATACGTT207.033528E-445.011
AATGTCG253.8910053E-545.01
GTTTACG302.1656579E-644.9999961
ACGGGTA1050.042.8571475
GCGATAA900.042.59
GCTACGA700.041.78571710
CGTTTTT46950.041.2619821
CTACGGG1800.041.253
TAGTATG1750.041.142861
GTATGCG501.0822987E-940.51
GACCGAT9650.040.336799
TAACGCC950.040.26315712
Produced by FastQC (version 0.11.5)