FastQCFastQC Report
Thu 26 May 2016
SRR1546593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546593_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1298616
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60470.46564958386466826No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG43020.33127575819179805No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC40370.31086941790336786No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC24440.18820036099971046No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22850.17595655682665237No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC16740.12890646657672478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG453.8562575E-1045.01
CGTAAAT207.0336263E-445.016
TTCGACT207.0336263E-445.024
ACGTGGT302.1657215E-644.99999613
CTAGCGG950.042.631582
TCACGAC1600.042.187525
CGTTCAT750.042.017
TGCGTTG650.041.538461
CGACGGT1600.040.7812528
CGTTTTT44250.040.7796631
CGGTCTA1550.040.64516431
TTTACGG1450.040.3448262
TTCGGCA1250.039.60000218
ATTTACG800.039.3751
TTAGGCG403.458681E-739.3751
AGGTACG403.458681E-739.3751
CCGATCG1200.039.37499640
ACGGGTA1100.038.8636365
GGGCGAT25150.038.737577
AACCCGA356.2489144E-638.5714324
Produced by FastQC (version 0.11.5)