FastQCFastQC Report
Thu 26 May 2016
SRR1546592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546592_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2128601
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328161.5416698573382235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT203250.043.2177121
GCGATAC700.041.7857179
TCACGAC1800.041.2525
CTATACG501.0822987E-940.4999961
CGGTCTA1850.038.9189231
GGCGATA8350.038.5329368
TACGGGT2400.038.4375044
GCGATAT2050.038.4146359
TAGGGCG8700.037.758625
TACGGGA4700.037.3404274
AGGGCGA22050.036.9387746
GTTTTTT238050.036.8714562
CGCGAAA552.752131E-936.8181836
GGGCGAT43050.036.6898967
TAGCGGG11500.036.1956523
TTACGGG4850.036.185573
TAACGCC1000.035.99999612
CGGTATG250.002107511535.9999961
CGAGGGT4950.035.9090924
GCGTAAG1450.035.6896551
Produced by FastQC (version 0.11.5)