FastQCFastQC Report
Thu 26 May 2016
SRR1546591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546591_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3001620
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276320.92056955910475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC3550.042.46478725
CGTTTTT151600.041.6457751
CGGTCTA3700.039.52702731
GCGTAAG2150.038.7209321
ACGACGG3850.038.5714327
TAACGGG7000.037.928573
TGATTCG6700.037.61194215
TAGGGAC21450.037.552455
GGGCGAT61800.037.063117
CGACGGT3950.037.02531428
AGGGCGA33100.036.84296
CACGACG4050.036.66666826
CATATGC16950.036.6371733
CGTAAGG4000.036.56252
GTATGCG1300.036.3461531
ATAGGGC16400.036.2195134
GGCGATT15850.036.198748
ATGGGAC15050.036.17945
TAGGGCG13450.036.1338275
CGTTCGG12300.036.03658745
Produced by FastQC (version 0.11.5)