##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546591_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3001620
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42767039132202	31.0	31.0	33.0	30.0	34.0
2	31.78485651081749	31.0	31.0	34.0	30.0	34.0
3	31.855342448411193	31.0	31.0	34.0	30.0	34.0
4	35.63808610017257	37.0	35.0	37.0	33.0	37.0
5	35.575012160100215	37.0	35.0	37.0	33.0	37.0
6	35.62161366195588	37.0	35.0	37.0	33.0	37.0
7	35.99005403748642	37.0	35.0	37.0	35.0	37.0
8	34.660651248325905	37.0	35.0	37.0	33.0	37.0
9	36.93688141736796	39.0	37.0	39.0	33.0	39.0
10	36.88326037273206	39.0	37.0	39.0	32.0	39.0
11	36.8482326210513	39.0	37.0	39.0	32.0	39.0
12	36.60448291256055	39.0	35.0	39.0	32.0	39.0
13	36.53603853918884	39.0	35.0	39.0	32.0	39.0
14	37.629613675282016	40.0	36.0	41.0	32.0	41.0
15	37.77714933935675	40.0	36.0	41.0	33.0	41.0
16	37.827792991784435	40.0	36.0	41.0	33.0	41.0
17	37.71406273945403	40.0	36.0	41.0	33.0	41.0
18	37.639814167016475	39.0	36.0	41.0	33.0	41.0
19	37.54568433046155	39.0	36.0	41.0	33.0	41.0
20	37.384880497864486	39.0	35.0	41.0	32.0	41.0
21	37.28610783510238	39.0	35.0	41.0	32.0	41.0
22	37.20041844070868	39.0	35.0	41.0	32.0	41.0
23	37.12026572317615	39.0	35.0	41.0	32.0	41.0
24	36.98679413116917	39.0	35.0	41.0	32.0	41.0
25	36.869180975606504	39.0	35.0	41.0	31.0	41.0
26	36.76898474823595	38.0	35.0	41.0	31.0	41.0
27	36.6449930370933	38.0	35.0	41.0	31.0	41.0
28	36.66040937893538	38.0	35.0	41.0	31.0	41.0
29	36.575899347685585	38.0	35.0	41.0	31.0	41.0
30	36.505109241009855	38.0	35.0	41.0	31.0	41.0
31	36.36203783290357	38.0	35.0	41.0	30.0	41.0
32	36.25918603953865	38.0	35.0	41.0	30.0	41.0
33	36.063881170834414	38.0	35.0	41.0	30.0	41.0
34	35.89543846322986	38.0	35.0	41.0	29.0	41.0
35	35.73610783510238	38.0	35.0	41.0	28.0	41.0
36	35.6292058954831	38.0	35.0	41.0	27.0	41.0
37	35.59040984535018	38.0	35.0	41.0	27.0	41.0
38	35.495718978418324	38.0	35.0	40.0	27.0	41.0
39	35.4139727880278	38.0	35.0	40.0	27.0	41.0
40	35.275492900500396	38.0	34.0	40.0	26.0	41.0
41	35.182219268261804	38.0	34.0	40.0	26.0	41.0
42	35.10086619891925	38.0	34.0	40.0	26.0	41.0
43	34.99626335112373	38.0	34.0	40.0	25.0	41.0
44	34.85868897462037	37.0	34.0	40.0	24.0	41.0
45	34.7277117023474	37.0	34.0	40.0	24.0	41.0
46	34.66151344940399	37.0	34.0	40.0	24.0	41.0
47	34.59004370973008	37.0	34.0	40.0	24.0	41.0
48	34.508218561976534	37.0	34.0	40.0	24.0	41.0
49	34.4561350204223	36.0	34.0	40.0	24.0	41.0
50	34.34148326570319	36.0	34.0	40.0	24.0	41.0
51	33.9716226570985	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	5.0
12	8.0
13	18.0
14	38.0
15	96.0
16	196.0
17	575.0
18	1159.0
19	2452.0
20	4214.0
21	6824.0
22	10535.0
23	15530.0
24	22950.0
25	33731.0
26	45656.0
27	53277.0
28	56986.0
29	61673.0
30	70763.0
31	85355.0
32	104880.0
33	134737.0
34	211377.0
35	304766.0
36	249901.0
37	320801.0
38	456386.0
39	746147.0
40	577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.13182881244128	23.291156109034457	26.33721123926413	15.239803839260132
2	34.94509631465675	23.98254942331141	27.543992910495003	13.528361351536835
3	29.565501295966847	24.97261478801447	31.884515694858113	13.577368221160574
4	26.042570345346842	27.205075925666804	31.225671470739137	15.526682258247213
5	22.88754072800688	31.197186852433017	29.94356380887654	15.971708610683564
6	20.956117030137058	40.4188071774575	27.864219987873216	10.76085580453222
7	83.76626621624322	4.214790679699629	9.667146407606559	2.351796696450583
8	80.6157008548717	6.595271886514616	9.241309692765906	3.5477175658477758
9	77.0893717392608	5.717745750627994	12.525336318388069	4.667546191723136
10	39.36447651601468	25.731538302649902	19.070335352243124	15.83364982909229
11	31.46161073020569	24.545412144108848	26.58874207927719	17.404235046408274
12	27.90679699628867	21.447751547497685	31.38948301250658	19.255968443707065
13	23.955697256814652	25.129463423084868	32.921055963113254	17.99378335698723
14	20.012793091730465	28.680445892551354	32.71173566274212	18.595025352976062
15	18.35458852219801	23.470359339290116	39.410385058734946	18.76466707977692
16	21.735029750601342	21.12562549556573	36.65250764587123	20.4868371079617
17	21.043803013039625	22.620818091563887	30.893284293148366	25.442094602248122
18	23.694438336631553	22.507279402456007	32.60089551642113	21.197386744491308
19	26.436057862087807	24.95432466468107	27.972594798808643	20.63702267442248
20	27.4089658251211	23.956863293821336	26.895043343261303	21.739127537796257
21	25.094082528767796	25.653780291975664	29.372005783543553	19.880131395712983
22	23.765300071294835	22.70074159953625	28.835861967870684	24.698096361298234
23	22.25101778373012	26.0922102064885	28.032995515754827	23.623776494026558
24	22.691479934168882	24.346919330228342	31.984561670031514	20.977039065571258
25	22.673123180149386	24.41531572950607	29.99303709330295	22.9185239970416
26	20.445892551355602	26.72070415309066	29.012333339996406	23.821069955557334
27	21.35027085373898	25.36093842658298	30.353842258513737	22.934948461164304
28	21.302596597837166	25.227210639587955	31.52650901846336	21.943683744111514
29	21.791365995695656	24.91604533551882	30.407213438076774	22.885375230708753
30	23.190177304255702	25.582418827166663	30.27718365416009	20.95022021441755
31	25.869297246153742	25.246800061300227	27.243088732084676	21.64081396046135
32	26.14927938912987	25.3570738467894	27.35869297246154	21.134953791619193
33	24.478881404041815	25.407846429594688	27.384878832097336	22.728393334266165
34	22.080809696097443	26.178396998953897	29.142929484744904	22.597863820203756
35	21.596437923521297	25.719678040524784	30.080156715373697	22.60372732058022
36	24.886094842118588	26.111932889572962	27.520438962960004	21.481533305348446
37	22.16636349704493	26.991424630699424	28.82756644745171	22.014645424803938
38	22.699075832383848	27.19318234819864	27.606892278169788	22.500849541247725
39	21.97603294221121	25.616833576535335	27.670524583391636	24.73660889786182
40	23.454034821196554	24.0638055450057	29.283120448291257	23.199039185506493
41	20.598110353742314	23.90822289297113	29.38786388683444	26.105802866452116
42	23.1693218995076	25.744398025066463	27.328609217689113	23.757670857736823
43	22.6598303582732	24.404055143555816	28.804512230062436	24.131602268108555
44	23.050552701541168	25.69309239677241	28.15436331047901	23.101991591207415
45	21.24522757710836	26.565221447085243	28.454867704772756	23.734683271033642
46	23.63543686409339	25.599109814033756	28.112319347552322	22.653133974320532
47	22.299858076638614	25.69086026878819	29.382000386457978	22.62728126811522
48	22.00701621124593	25.06743025432933	29.96242029304176	22.963133241382984
49	22.890705685596444	23.67428255408746	29.41511583744778	24.01989592286832
50	21.582778632871584	25.312531233134106	30.095415142489724	23.00927499150459
51	21.73626241829412	26.33617846362964	28.006176664601117	23.921382453475122
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3275.0
1	4669.5
2	6064.0
3	25705.5
4	45347.0
5	30872.0
6	16397.0
7	16543.0
8	16689.0
9	17493.0
10	18297.0
11	18198.5
12	18100.0
13	17801.5
14	17503.0
15	16814.0
16	16125.0
17	15853.0
18	15581.0
19	14865.5
20	14150.0
21	14049.0
22	13948.0
23	15133.5
24	16319.0
25	18194.0
26	21405.0
27	22741.0
28	27259.5
29	31778.0
30	35621.5
31	39465.0
32	43775.0
33	48085.0
34	54900.5
35	61716.0
36	71088.0
37	80460.0
38	88459.0
39	96458.0
40	114882.5
41	133307.0
42	157071.0
43	180835.0
44	202918.5
45	225002.0
46	242527.5
47	260053.0
48	254248.5
49	248444.0
50	228905.0
51	209366.0
52	197118.0
53	184870.0
54	173096.5
55	161323.0
56	155392.0
57	149461.0
58	142625.0
59	135789.0
60	129000.5
61	122212.0
62	112147.5
63	102083.0
64	93213.5
65	84344.0
66	72657.5
67	60971.0
68	52447.0
69	43923.0
70	37577.0
71	31231.0
72	25864.0
73	20497.0
74	17499.0
75	11095.0
76	7689.0
77	5859.0
78	4029.0
79	2778.0
80	1527.0
81	1239.5
82	952.0
83	678.0
84	404.0
85	276.0
86	148.0
87	105.5
88	63.0
89	38.5
90	14.0
91	9.5
92	5.0
93	5.5
94	6.0
95	5.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3001620.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.37290218108332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.13361695526203	19.287159052859177
2	7.236223418489699	3.5273553107862754
3	2.4690892624114555	1.8053661320735275
4	1.3713532962643813	1.3369543898223175
5	0.8740305883428606	1.0651331016477623
6	0.6552943897571648	0.9582855636818439
7	0.5170508801924738	0.8821421367901937
8	0.4455331604123807	0.8687148909727888
9	0.3482487190324811	0.7639048767299608
>10	5.003916491865564	30.079826183633386
>50	1.6939259537940454	28.515238895246082
>100	0.2437908787687865	8.042166010747877
>500	0.004953753379165291	0.8756562298746782
>1k	0.0028307162166658806	1.0388931223962263
>5k	0.0	0.0
>10k+	1.4153581083329404E-4	0.9532041027378313
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27632	0.92056955910475	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.663068609617474E-5	0.0	0.0	0.09161719338224025	0.0
2	6.663068609617474E-5	0.0	0.0	0.28777793324937867	0.0
3	6.663068609617474E-5	0.0	0.0	0.4740773315742832	0.0
4	6.663068609617474E-5	0.0	0.0	0.7598896595838247	0.0
5	6.663068609617474E-5	0.0	0.0	1.2930017790393187	0.0
6	6.663068609617474E-5	0.0	0.0	2.1908502741852733	0.0
7	6.663068609617474E-5	0.0	0.0	2.658930844010901	0.0
8	6.663068609617474E-5	0.0	0.0	3.806344573930078	0.0
9	6.663068609617474E-5	0.0	0.0	4.443433879038652	0.0
10	6.663068609617474E-5	0.0	0.0	5.39418713894497	0.0
11	6.663068609617474E-5	0.0	0.0	6.041670831084548	0.0
12	6.663068609617474E-5	0.0	0.0	6.605966111633052	0.0
13	6.663068609617474E-5	0.0	0.0	6.871022980923635	0.0
14	6.663068609617474E-5	0.0	0.0	6.995189264463856	0.0
15	6.663068609617474E-5	0.0	0.0	7.130416241896043	0.0
16	6.663068609617474E-5	0.0	0.0	7.379481746523544	0.0
17	6.663068609617474E-5	0.0	0.0	7.65040211619059	0.0
18	6.663068609617474E-5	0.0	0.0	8.06514482179623	0.0
19	6.663068609617474E-5	0.0	0.0	8.255941791432626	0.0
20	6.663068609617474E-5	0.0	0.0	8.459665114171681	0.0
21	6.663068609617474E-5	0.0	0.0	8.686575915672204	0.0
22	6.663068609617474E-5	0.0	0.0	8.923481320087152	0.0
23	6.663068609617474E-5	0.0	0.0	9.197100232541095	0.0
24	6.663068609617474E-5	0.0	0.0	9.415648882936548	0.0
25	6.663068609617474E-5	0.0	0.0	9.596651141716807	0.0
26	6.663068609617474E-5	0.0	0.0	9.764427209306975	0.0
27	6.663068609617474E-5	0.0	0.0	9.962953338530527	0.0
28	6.663068609617474E-5	0.0	0.0	10.149019529454094	0.0
29	6.663068609617474E-5	0.0	0.0	10.357906730365602	0.0
30	6.663068609617474E-5	0.0	0.0	10.628493946602168	0.0
31	9.99460291442621E-5	0.0	0.0	10.839180176038273	0.0
32	9.99460291442621E-5	0.0	0.0	11.054264030756725	0.0
33	9.99460291442621E-5	0.0	0.0	11.273778826100573	0.0
34	9.99460291442621E-5	0.0	0.0	11.497857823442008	0.0
35	9.99460291442621E-5	0.0	0.0	11.743725055136892	0.0
36	9.99460291442621E-5	0.0	0.0	11.95157948041391	0.0
37	9.99460291442621E-5	0.0	0.0	12.164797675921669	0.0
38	9.99460291442621E-5	0.0	0.0	12.406500489735542	0.0
39	9.99460291442621E-5	0.0	0.0	12.722429887860555	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	355	0.0	42.464787	25
CGTTTTT	15160	0.0	41.645775	1
CGGTCTA	370	0.0	39.527027	31
GCGTAAG	215	0.0	38.720932	1
ACGACGG	385	0.0	38.57143	27
TAACGGG	700	0.0	37.92857	3
TGATTCG	670	0.0	37.611942	15
TAGGGAC	2145	0.0	37.55245	5
GGGCGAT	6180	0.0	37.06311	7
CGACGGT	395	0.0	37.025314	28
AGGGCGA	3310	0.0	36.8429	6
CACGACG	405	0.0	36.666668	26
CATATGC	1695	0.0	36.63717	33
CGTAAGG	400	0.0	36.5625	2
GTATGCG	130	0.0	36.346153	1
ATAGGGC	1640	0.0	36.219513	4
GGCGATT	1585	0.0	36.19874	8
ATGGGAC	1505	0.0	36.1794	5
TAGGGCG	1345	0.0	36.133827	5
CGTTCGG	1230	0.0	36.036587	45
>>END_MODULE