FastQCFastQC Report
Thu 26 May 2016
SRR1546590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546590_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1372260
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60110.4380365236908458No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36330.2647457478903415No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT18070.13168058531182136RNA PCR Primer, Index 16 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA406.8175723E-945.012
CGTATCG207.033766E-445.035
CTATCGG253.891203E-545.02
CGTGAGC207.033766E-445.033
CCTGTCG253.891203E-545.017
TCGATAG406.8175723E-945.01
ACTACGG207.033766E-445.02
CGAATCG207.033766E-445.037
CGTCGTT207.033766E-445.037
CGACTAA207.033766E-445.013
GTGACCG207.033766E-445.017
TCGACAG253.891203E-545.01
TATCGTG253.891203E-545.01
GCCGTAT207.033766E-445.042
AATCGTC207.033766E-445.039
CGCATCG302.1658125E-644.99999621
ACGGATC302.1658125E-644.99999641
TGCACGG1300.041.5384642
GTAGGGT6000.040.8754
TTTAGCG501.0822987E-940.51