FastQCFastQC Report
Thu 26 May 2016
SRR1546589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546589_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1937246
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243161.2551839053997271No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24820.12812002192803598No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA207.034485E-445.00000444
GCCGAAC302.1662763E-644.99999643
CGTTTTT122550.042.631581
TATCGCG556.184564E-1140.9090921
TCACGAC2650.040.7547225
TAACGGG4800.040.3124963
ATACTAT19200.040.31249645
ACGGGTA5750.040.3043485
CGCGATA451.929402E-840.016
TCGCTAT900.040.023
GCTAACG451.929402E-840.01
ATGTTCG451.929402E-840.01
CGACGGT2650.039.90566328
ACGGGAT4800.039.8437465
GCGATCG850.039.7058839
CGGGTCC2950.039.661026
ATAGGGT17250.039.6521764
GCGATAC2000.039.3759
CGTTCGA1200.039.37499614
CGTTCGG7900.039.30379545
Produced by FastQC (version 0.11.5)