##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546589_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1937246
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.356185017287427	31.0	31.0	33.0	30.0	34.0
2	31.722254685259383	31.0	31.0	34.0	30.0	34.0
3	31.802491784729455	31.0	31.0	34.0	30.0	34.0
4	35.60766934090972	37.0	35.0	37.0	33.0	37.0
5	35.54532516778974	37.0	35.0	37.0	33.0	37.0
6	35.59921042552159	37.0	35.0	37.0	33.0	37.0
7	35.996058838165105	37.0	35.0	37.0	35.0	37.0
8	34.692661644416866	37.0	35.0	37.0	33.0	37.0
9	36.974616027081744	39.0	37.0	39.0	33.0	39.0
10	36.633388841685566	39.0	35.0	39.0	32.0	39.0
11	36.762919629205584	39.0	35.0	39.0	32.0	39.0
12	36.52505928519145	39.0	35.0	39.0	32.0	39.0
13	36.40828010484987	39.0	35.0	39.0	32.0	39.0
14	37.50974114800082	40.0	36.0	41.0	32.0	41.0
15	37.69383960529535	40.0	36.0	41.0	33.0	41.0
16	37.76813063493227	40.0	36.0	41.0	33.0	41.0
17	37.6702845172993	40.0	35.0	41.0	33.0	41.0
18	37.5927404160339	39.0	36.0	41.0	33.0	41.0
19	37.49517356081778	39.0	36.0	41.0	33.0	41.0
20	37.315221195449624	39.0	35.0	41.0	32.0	41.0
21	37.19525140328074	39.0	35.0	41.0	32.0	41.0
22	37.11135085580251	39.0	35.0	41.0	32.0	41.0
23	37.053916745730795	39.0	35.0	41.0	32.0	41.0
24	36.922435250866435	38.0	35.0	41.0	32.0	41.0
25	36.835780793972475	38.0	35.0	41.0	32.0	41.0
26	36.74664549571918	38.0	35.0	41.0	31.0	41.0
27	36.655462961337896	38.0	35.0	41.0	31.0	41.0
28	36.666908074658565	38.0	35.0	41.0	31.0	41.0
29	36.62405548908089	38.0	35.0	41.0	31.0	41.0
30	36.568656226416266	38.0	35.0	41.0	31.0	41.0
31	36.449000281843404	38.0	35.0	41.0	31.0	41.0
32	36.280590590972956	38.0	35.0	41.0	30.0	41.0
33	36.107443246753384	38.0	35.0	41.0	30.0	41.0
34	35.90852839546449	38.0	35.0	41.0	29.0	41.0
35	35.76640292456405	38.0	35.0	41.0	29.0	41.0
36	35.61804644324985	38.0	35.0	41.0	27.0	41.0
37	35.56523487466228	38.0	35.0	41.0	27.0	41.0
38	35.4774886617394	38.0	35.0	41.0	27.0	41.0
39	35.4219897731109	38.0	35.0	40.0	27.0	41.0
40	35.29655913601061	38.0	35.0	40.0	26.0	41.0
41	35.23735550363764	38.0	35.0	40.0	25.0	41.0
42	35.18026827775099	38.0	35.0	40.0	25.0	41.0
43	35.09422293296773	38.0	34.0	40.0	25.0	41.0
44	34.974665581965326	38.0	34.0	40.0	25.0	41.0
45	34.856399755116286	38.0	34.0	40.0	24.0	41.0
46	34.81039217528388	37.0	34.0	40.0	24.0	41.0
47	34.742456559466376	37.0	34.0	40.0	24.0	41.0
48	34.68855168625977	37.0	34.0	40.0	24.0	41.0
49	34.6334838218791	37.0	34.0	40.0	24.0	41.0
50	34.5254665643909	37.0	34.0	40.0	24.0	41.0
51	34.18152418433178	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	5.0
14	18.0
15	46.0
16	122.0
17	320.0
18	776.0
19	1502.0
20	2561.0
21	4455.0
22	6749.0
23	10033.0
24	14947.0
25	23001.0
26	31478.0
27	35955.0
28	36356.0
29	38379.0
30	44169.0
31	53435.0
32	66438.0
33	85820.0
34	135738.0
35	218016.0
36	150780.0
37	186594.0
38	279556.0
39	509563.0
40	429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.157275844162285	23.299209289888843	26.829065591050387	16.71444927489849
2	33.5815895348345	24.364897385257215	28.998588718211316	13.054924361696965
3	27.683629234490613	24.628209323957826	34.28712718983547	13.401034251716096
4	24.10741846931159	27.405863788078538	32.87729075192309	15.609426990686778
5	21.476518728132618	31.4033426833763	32.16137754317211	14.958761045318974
6	18.905084847252233	40.55277440242489	29.468740676197037	11.073400074125846
7	85.29923406733063	3.3912058664723013	9.6708420097396	1.638718056457466
8	83.10870173431769	5.336544765094366	8.8371327131402	2.717620787447748
9	79.76457300724843	3.8864966039418842	13.006040533829982	3.3428898549796977
10	32.988634380971746	21.64474723395996	31.326635853164753	14.039982531903537
11	32.93727280892566	23.562779326941442	25.864397190651054	17.63555067348184
12	29.697364196390136	22.558776737698775	29.344853467241638	18.39900559866945
13	21.687178603027185	30.976912586217754	29.969916056092	17.36599275466306
14	18.118504309726283	33.976376774039025	29.610281812428575	18.294837103806124
15	16.31016401634072	25.280733577459962	40.66029817586409	17.748804230335228
16	17.994926818793278	21.659975036727396	39.88605473956328	20.45904340491605
17	17.18377531815784	23.199325227668556	32.097317532208095	27.519581921965514
18	20.694532341272094	23.369050703937443	34.763783226291345	21.172633728499115
19	23.41850234817881	27.312174086306023	28.03866932748861	21.23065423802656
20	26.237710646969976	24.39514651211049	27.636397236076366	21.730745604843165
21	21.713298156248612	27.748360301169804	29.548338207950874	20.990003334630707
22	21.05396010625393	24.593572525120713	28.343277002507683	26.009190366117675
23	18.86595713709049	29.25235101788828	28.016782587239824	23.86490925778141
24	19.238857636046223	25.961080833306667	35.85801699938986	18.94204453125726
25	19.73564534395735	26.89787461169103	31.631346767524622	21.735133276827
26	18.19350769081469	29.344543749219255	29.6981384914461	22.763810068519952
27	18.868641359951187	28.186249965156723	29.56537269918224	23.37973597570985
28	16.61007430135357	27.45908366825896	34.190546786520656	21.740295243866807
29	17.419986929899455	25.253065434126594	33.66041277153237	23.666534864441584
30	19.693007496208534	27.207902352101904	32.650732018545916	20.44835813314365
31	23.611714774478823	26.904636788513177	26.951817167257026	22.531831269750977
32	23.12421860723935	26.914857483251996	26.97447820256178	22.986445706946874
33	21.828668119588322	27.49351398841448	26.040988083082894	24.636829808914303
34	18.696644618184784	27.255650547220128	29.397918488410866	24.64978634618422
35	18.43993999729513	25.667055190719196	30.64866310215636	25.244341709829314
36	23.385207660772046	28.19791601066669	26.981395238395123	21.435481090166142
37	18.920880466394046	29.72214163818121	29.693802439132668	21.663175456292077
38	19.73058661625834	30.729964083033334	28.285359732321037	21.25408956838729
39	19.379882575573777	30.06809666918915	27.576518418414597	22.97550233682248
40	21.680468045875433	27.171458864800858	28.962816286625447	22.185256802698262
41	17.472174416671916	26.192078858338075	29.68895019011525	26.646796534874767
42	20.329839369909656	28.38534703388212	26.965444760242118	24.31936883596611
43	20.42172238321824	26.99032544137399	27.810045807295513	24.77790636811226
44	20.70594028843007	28.196212561543554	27.95953637276835	23.138310777258024
45	18.768292720697318	30.314993552703168	26.396183035092086	24.520530691507428
46	21.52917079193866	28.775694981432405	28.12043488539917	21.574699341229767
47	20.594957997074196	28.675088243826547	28.96054502112793	21.769408737971325
48	21.150953466931924	26.110726257790702	29.426206067789018	23.312114207488364
49	20.496261187273067	25.278513931632844	29.999132789537313	24.22609209155678
50	19.497472184740605	27.68987521460878	30.6019473004461	22.210705300204516
51	18.85511700630689	28.495555030182025	28.04889002222743	24.600437941283655
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2125.0
1	2609.5
2	3094.0
3	19549.0
4	36004.0
5	23336.5
6	10669.0
7	10676.5
8	10684.0
9	11516.0
10	12348.0
11	12848.5
12	13349.0
13	13699.5
14	14050.0
15	13925.0
16	13800.0
17	13166.5
18	12533.0
19	11818.0
20	11103.0
21	10661.5
22	10220.0
23	10132.5
24	10045.0
25	11145.5
26	14332.0
27	16418.0
28	19126.0
29	21834.0
30	24917.0
31	28000.0
32	33693.5
33	39387.0
34	44557.0
35	49727.0
36	53038.5
37	56350.0
38	63797.0
39	71244.0
40	86170.0
41	101096.0
42	115895.5
43	130695.0
44	142516.0
45	154337.0
46	166560.5
47	178784.0
48	180911.0
49	183038.0
50	175415.5
51	167793.0
52	151613.0
53	135433.0
54	122628.5
55	109824.0
56	100026.0
57	90228.0
58	80226.0
59	70224.0
60	61307.5
61	52391.0
62	45388.5
63	38386.0
64	32551.0
65	26716.0
66	21288.5
67	15861.0
68	12489.5
69	9118.0
70	7283.0
71	5448.0
72	5042.0
73	4636.0
74	4310.0
75	3181.0
76	2378.0
77	1665.0
78	952.0
79	662.5
80	373.0
81	277.5
82	182.0
83	124.0
84	66.0
85	56.0
86	46.0
87	29.0
88	12.0
89	10.5
90	9.0
91	5.0
92	1.0
93	2.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1937246.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.840069720231263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.898393085008	20.128999084105875
2	7.353247090716408	3.8001683498839935
3	2.807077123042221	2.176052057084315
4	1.5718061778496355	1.624623248892992
5	1.0491112247244103	1.3554553595577985
6	0.7724147780185004	1.1975551030161002
7	0.6158961245220416	1.1140359158648854
8	0.5087925589180918	1.0517788156462693
9	0.4263941238433688	0.9916248499568615
>10	5.466482789736832	33.128336967507025
>50	1.3408406606799939	23.76143045823342
>100	0.18127625342063847	6.536501575405164
>500	0.005787606663972895	1.0511380368212093
>1k	0.0022737026179893514	0.7835464939109318
>5k	0.0	0.0
>10k+	2.0670023799903192E-4	1.298753684113035
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24316	1.2551839053997271	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2482	0.12812002192803598	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5485901119424172E-4	0.0	0.0	0.08223013494414236	0.0
2	1.5485901119424172E-4	0.0	0.0	0.26320869935981284	0.0
3	1.5485901119424172E-4	0.0	0.0	0.4220940448451049	0.0
4	1.5485901119424172E-4	0.0	0.0	0.6692490267111146	0.0
5	1.5485901119424172E-4	0.0	0.0	1.1327936668858782	0.0
6	1.5485901119424172E-4	0.0	0.0	1.9763107008609129	0.0
7	1.5485901119424172E-4	0.0	0.0	2.467936441732232	0.0
8	1.5485901119424172E-4	0.0	0.0	3.69271636126749	0.0
9	1.5485901119424172E-4	0.0	0.0	4.3113781109884854	0.0
10	1.5485901119424172E-4	0.0	0.0	5.273000950834328	0.0
11	1.5485901119424172E-4	0.0	0.0	5.8362231745477855	0.0
12	1.5485901119424172E-4	0.0	0.0	6.336779118397973	0.0
13	1.5485901119424172E-4	0.0	0.0	6.577223543112233	0.0
14	1.5485901119424172E-4	0.0	0.0	6.684850555892231	0.0
15	1.5485901119424172E-4	0.0	0.0	6.805279246930953	0.0
16	1.5485901119424172E-4	0.0	0.0	7.049027330550689	0.0
17	1.5485901119424172E-4	0.0	0.0	7.30475117770278	0.0
18	1.5485901119424172E-4	0.0	0.0	7.706094115047857	0.0
19	1.5485901119424172E-4	0.0	0.0	7.855842778872688	5.161967039808058E-5
20	1.5485901119424172E-4	0.0	0.0	8.033104727019698	5.161967039808058E-5
21	1.5485901119424172E-4	0.0	0.0	8.256153322809803	5.161967039808058E-5
22	1.5485901119424172E-4	0.0	0.0	8.49210683619943	5.161967039808058E-5
23	1.5485901119424172E-4	0.0	0.0	8.765794328650053	5.161967039808058E-5
24	1.5485901119424172E-4	0.0	0.0	8.974905613432677	5.161967039808058E-5
25	1.5485901119424172E-4	0.0	0.0	9.15960079411701	5.161967039808058E-5
26	1.5485901119424172E-4	0.0	0.0	9.340837456884671	5.161967039808058E-5
27	1.5485901119424172E-4	0.0	0.0	9.531830237357568	5.161967039808058E-5
28	1.5485901119424172E-4	0.0	0.0	9.725507240691167	5.161967039808058E-5
29	1.5485901119424172E-4	0.0	0.0	9.959292727924074	5.161967039808058E-5
30	1.5485901119424172E-4	0.0	0.0	10.252131118092384	5.161967039808058E-5
31	1.5485901119424172E-4	0.0	0.0	10.46201669793098	5.161967039808058E-5
32	1.5485901119424172E-4	0.0	0.0	10.674380021948684	5.161967039808058E-5
33	1.5485901119424172E-4	0.0	0.0	10.880600605189017	5.161967039808058E-5
34	1.5485901119424172E-4	0.0	0.0	11.086098513043774	5.161967039808058E-5
35	1.5485901119424172E-4	0.0	0.0	11.333201875239386	5.161967039808058E-5
36	1.5485901119424172E-4	0.0	0.0	11.54473928453072	5.161967039808058E-5
37	1.5485901119424172E-4	0.0	0.0	11.755141061073298	5.161967039808058E-5
38	1.5485901119424172E-4	0.0	0.0	11.977415361807433	5.161967039808058E-5
39	1.5485901119424172E-4	0.0	0.0	12.242740467653565	5.161967039808058E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGA	20	7.034485E-4	45.000004	44
GCCGAAC	30	2.1662763E-6	44.999996	43
CGTTTTT	12255	0.0	42.63158	1
TATCGCG	55	6.184564E-11	40.909092	1
TCACGAC	265	0.0	40.75472	25
TAACGGG	480	0.0	40.312496	3
ATACTAT	1920	0.0	40.312496	45
ACGGGTA	575	0.0	40.304348	5
CGCGATA	45	1.929402E-8	40.0	16
TCGCTAT	90	0.0	40.0	23
GCTAACG	45	1.929402E-8	40.0	1
ATGTTCG	45	1.929402E-8	40.0	1
CGACGGT	265	0.0	39.905663	28
ACGGGAT	480	0.0	39.843746	5
GCGATCG	85	0.0	39.705883	9
CGGGTCC	295	0.0	39.66102	6
ATAGGGT	1725	0.0	39.652176	4
GCGATAC	200	0.0	39.375	9
CGTTCGA	120	0.0	39.374996	14
CGTTCGG	790	0.0	39.303795	45
>>END_MODULE