SRR1546588_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546588_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1631920
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7203	0.44138193048678853	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4717	0.289046031668219	No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	3919	0.24014657581253981	TruSeq Adapter, Index 27 (100% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3623	0.22200843178587187	TruSeq Adapter, Index 27 (96% over 25bp)
GAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	3166	0.19400460806902298	TruSeq Adapter, Index 27 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2947	0.18058483258983285	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2879	0.1764179616647875	TruSeq Adapter, Index 27 (96% over 25bp)
AAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	2787	0.17078043041325555	TruSeq Adapter, Index 27 (100% over 22bp)
TAGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	1831	0.1121991274082063	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	1715	0.10509093583018776	No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1694	0.103804108044512	TruSeq Adapter, Index 27 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2123564E-7	45.0	1
TAATAGT	40	6.8175723E-9	45.0	35
CTCGTGA	20	7.034159E-4	45.0	32
GTCGACG	35	1.2123564E-7	45.0	1
CAACGAA	20	7.034159E-4	45.0	22
GCGGTAT	25	3.8915285E-5	45.0	28
GACGTAT	20	7.034159E-4	45.0	9
CGCGCGA	20	7.034159E-4	45.0	14
CGACAAC	20	7.034159E-4	45.0	36
TATGCGT	20	7.034159E-4	45.0	10
GTAATCG	55	1.8189894E-12	45.0	1
CGTTAGT	20	7.034159E-4	45.0	12
TGCGCAC	20	7.034159E-4	45.0	28
CGGATTA	25	3.8915285E-5	45.0	20
CGAATCG	25	3.8915285E-5	45.0	36
GCGATCG	20	7.034159E-4	45.0	9
CTACCGT	20	7.034159E-4	45.0	11
ATGCGTA	20	7.034159E-4	45.0	26
CGTAAAC	20	7.034159E-4	45.0	35
CGTCGAA	20	7.034159E-4	45.0	23