FastQCFastQC Report
Thu 26 May 2016
SRR1546588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546588_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1631920
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72030.44138193048678853No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47170.289046031668219No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT39190.24014657581253981TruSeq Adapter, Index 27 (100% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC36230.22200843178587187TruSeq Adapter, Index 27 (96% over 25bp)
GAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT31660.19400460806902298TruSeq Adapter, Index 27 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29470.18058483258983285No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC28790.1764179616647875TruSeq Adapter, Index 27 (96% over 25bp)
AAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT27870.17078043041325555TruSeq Adapter, Index 27 (100% over 22bp)
TAGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT18310.1121991274082063No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT17150.10509093583018776No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC16940.103804108044512TruSeq Adapter, Index 27 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG351.2123564E-745.01
TAATAGT406.8175723E-945.035
CTCGTGA207.034159E-445.032
GTCGACG351.2123564E-745.01
CAACGAA207.034159E-445.022
GCGGTAT253.8915285E-545.028
GACGTAT207.034159E-445.09
CGCGCGA207.034159E-445.014
CGACAAC207.034159E-445.036
TATGCGT207.034159E-445.010
GTAATCG551.8189894E-1245.01
CGTTAGT207.034159E-445.012
TGCGCAC207.034159E-445.028
CGGATTA253.8915285E-545.020
CGAATCG253.8915285E-545.036
GCGATCG207.034159E-445.09
CTACCGT207.034159E-445.011
ATGCGTA207.034159E-445.026
CGTAAAC207.034159E-445.035
CGTCGAA207.034159E-445.023