##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546588_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1631920
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266567601353007	31.0	31.0	33.0	30.0	34.0
2	31.597108314133045	31.0	31.0	34.0	30.0	34.0
3	31.69132126574832	31.0	31.0	34.0	30.0	34.0
4	35.452370214226185	37.0	35.0	37.0	33.0	37.0
5	35.36322184911025	37.0	35.0	37.0	33.0	37.0
6	35.415796117456736	37.0	35.0	37.0	33.0	37.0
7	35.85609221040247	37.0	35.0	37.0	35.0	37.0
8	34.53075886072847	37.0	35.0	37.0	32.0	37.0
9	36.79480244129614	39.0	37.0	39.0	32.0	39.0
10	36.74799499975489	39.0	37.0	39.0	32.0	39.0
11	36.611696039021524	39.0	35.0	39.0	32.0	39.0
12	35.82681810382862	37.0	35.0	39.0	32.0	39.0
13	35.40743725182607	37.0	35.0	39.0	30.0	39.0
14	36.366275307613115	38.0	35.0	41.0	31.0	41.0
15	36.73166638070494	38.0	35.0	41.0	32.0	41.0
16	36.91560738271484	38.0	35.0	41.0	32.0	41.0
17	36.85143450659346	38.0	35.0	41.0	32.0	41.0
18	36.81275430168146	38.0	35.0	40.0	32.0	41.0
19	36.72158622971714	38.0	35.0	40.0	32.0	41.0
20	36.522327687631744	37.0	35.0	40.0	31.0	41.0
21	36.37799095543899	37.0	35.0	40.0	31.0	41.0
22	36.277987278788174	37.0	35.0	40.0	31.0	41.0
23	36.270744276680226	37.0	35.0	40.0	31.0	41.0
24	36.09822233933036	37.0	35.0	40.0	31.0	41.0
25	36.00258774939948	36.0	35.0	40.0	31.0	41.0
26	35.8487009167116	36.0	35.0	40.0	30.0	41.0
27	35.7529535761557	36.0	34.0	40.0	30.0	41.0
28	35.788559488210204	36.0	35.0	40.0	30.0	41.0
29	35.79426993970293	36.0	35.0	40.0	30.0	41.0
30	35.73664211480955	36.0	35.0	40.0	30.0	41.0
31	35.59719471542723	36.0	34.0	40.0	30.0	41.0
32	35.387773910485805	36.0	34.0	40.0	29.0	41.0
33	35.238751286827785	36.0	34.0	40.0	29.0	41.0
34	35.146880974557575	36.0	34.0	40.0	28.0	41.0
35	35.01545051228001	36.0	34.0	40.0	27.0	41.0
36	34.861398230305404	36.0	34.0	40.0	26.0	41.0
37	34.757118608755334	36.0	34.0	40.0	26.0	41.0
38	34.70558912201579	36.0	34.0	40.0	26.0	41.0
39	34.65041178489142	36.0	34.0	40.0	25.0	41.0
40	34.458108853375165	36.0	34.0	40.0	24.0	41.0
41	34.41527096916516	36.0	34.0	40.0	24.0	41.0
42	34.32337063091328	36.0	34.0	40.0	23.0	41.0
43	34.16791693220256	35.0	33.0	40.0	23.0	41.0
44	34.03719912740821	35.0	33.0	40.0	23.0	41.0
45	33.90124393352615	35.0	33.0	40.0	23.0	41.0
46	33.85903720770626	35.0	33.0	40.0	23.0	41.0
47	33.8057508946517	35.0	33.0	40.0	23.0	41.0
48	33.780456149811265	35.0	33.0	40.0	23.0	41.0
49	33.777616549830874	35.0	33.0	40.0	23.0	41.0
50	33.59517378302858	35.0	33.0	39.0	23.0	41.0
51	33.217504534536005	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	3.0
13	4.0
14	19.0
15	77.0
16	174.0
17	447.0
18	1063.0
19	1985.0
20	3258.0
21	5167.0
22	7692.0
23	11102.0
24	16045.0
25	22131.0
26	27998.0
27	31813.0
28	34650.0
29	38920.0
30	46018.0
31	54942.0
32	68345.0
33	87554.0
34	152197.0
35	270929.0
36	112172.0
37	132727.0
38	187463.0
39	316725.0
40	296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.82758958772489	24.789450463258003	28.456235599784304	13.926724349232805
2	29.969116133143782	27.090114711505464	29.807956272366294	13.13281288298446
3	28.372898181283396	26.275920388254328	31.140374528163147	14.210806902299133
4	24.408365606157165	29.139970096573357	30.837418500906903	15.614245796362566
5	22.17271679984313	31.983982057944015	31.104772292759446	14.738528849453406
6	19.703600666699348	41.04502671699593	27.3047085641453	11.94666405215942
7	82.20219128388646	5.649357811657434	9.446602774645815	2.7018481298102848
8	78.478234227168	7.439273984018824	8.143965390460316	5.9385263983528604
9	75.5282734447767	6.396759645080641	13.284352174126182	4.7906147360164715
10	49.478650914260506	21.26341977547919	15.563814402666798	13.69411490759351
11	43.998909260257854	20.18646747389578	21.82931761360851	13.985305652237855
12	37.98164125692436	20.326915535075248	25.4534536006667	16.23798960733369
13	20.024327172900634	40.747034168341585	26.397678807784697	12.830959850973086
14	13.488283739398991	43.47186136575322	27.58100887298397	15.458846021863817
15	10.893977645963037	23.723895779204863	51.69940928476886	13.682717290063238
16	12.212669738712682	19.176736604735524	47.75454679150939	20.856046865042405
17	11.922827099367616	21.442350115201727	29.13139124466886	37.5034315407618
18	18.555321339281335	22.99726702289328	36.71387077797931	21.73354085984607
19	25.10760331388794	27.85038482278543	25.708245502230504	21.33376636109613
20	27.31114270307368	24.35885337516545	25.24848031766263	23.08152360409824
21	18.95264473748713	31.969765674787983	27.703992842786413	21.373596744938478
22	19.295124761017696	26.55552968282759	24.785038482278544	29.364307073876173
23	16.774351683906076	32.76055198784254	23.40727486641502	27.05782146183636
24	18.297710672091767	24.794781606941516	38.279511250551494	18.627996470415216
25	15.604073729104368	26.4894725231629	34.44513211431933	23.461321633413405
26	14.947485170841707	34.07832491788813	27.961909897543997	23.012280013726162
27	17.912642776606695	32.903634982106965	26.815468895534096	22.368253345752244
28	13.924457081229471	29.5635202706015	36.56606941516741	19.945953233001617
29	13.780148536692973	25.293396735134078	35.03921760870631	25.88723711946664
30	17.547428795529193	29.743614883082504	30.40859846070886	22.300357860679444
31	25.787783714887986	27.856941516741017	23.040345114956615	23.31492965341438
32	25.838153831070148	26.29497769498505	24.8131648610226	23.053703612922202
33	22.960561792244718	28.572907985685575	24.824439923525663	23.642090298544048
34	16.20379675474288	25.816216481200062	29.652740330408356	28.327246433648707
35	16.339281337320458	24.444151674101672	31.02909456345899	28.187472425118877
36	26.439715182116768	22.20298789156331	29.469030344624734	21.88826658169518
37	17.387555762537378	28.94008284719839	31.367652826118924	22.3047085641453
38	17.82415804696309	29.783567821952055	27.75828471983921	24.63398941124565
39	17.93053581057895	27.430450022059905	29.209826462081473	25.42918770527967
40	21.805419383303104	22.179150938771507	30.013297220451985	26.002132457473405
41	14.783016324329623	22.380937791068188	29.189114662483455	33.64693122211873
42	21.546399333300652	24.801583410951515	25.566326780724545	28.085690475023284
43	20.854698759743126	24.070542673660473	28.213147703318793	26.861610863277612
44	19.401563802147166	28.53650914260503	27.936050786803275	24.125876268444532
45	16.516863571743713	32.86135349772047	25.089955389970097	25.531827540565715
46	22.514338938183247	28.33515123290357	28.3678734251679	20.78263640374528
47	21.02210892690818	24.790676013530074	28.509363204078632	25.677851855483112
48	21.352701112799647	21.24711995686063	31.596217951860382	25.803960978479335
49	20.569329378891123	19.692203049169077	32.92226334624247	26.816204225697337
50	18.267255747830777	27.543874699740183	30.011275062503064	24.177594489925976
51	16.847762145203195	28.42633217314574	26.80315211529977	27.922753566351293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3834.0
1	3852.0
2	3870.0
3	8017.5
4	12165.0
5	8995.0
6	5825.0
7	6688.5
8	7552.0
9	8770.0
10	9988.0
11	10522.0
12	11056.0
13	11060.5
14	11065.0
15	11025.0
16	10985.0
17	10871.5
18	10758.0
19	10199.5
20	9641.0
21	9424.0
22	9207.0
23	8758.5
24	8310.0
25	7975.5
26	8267.0
27	8893.0
28	8788.0
29	8683.0
30	10649.5
31	12616.0
32	15441.5
33	18267.0
34	23760.0
35	29253.0
36	31242.0
37	33231.0
38	41606.5
39	49982.0
40	73331.5
41	96681.0
42	122479.0
43	148277.0
44	161610.5
45	174944.0
46	184291.5
47	193639.0
48	192371.5
49	191104.0
50	175068.0
51	159032.0
52	132468.0
53	105904.0
54	88587.0
55	71270.0
56	62508.0
57	53746.0
58	48125.0
59	42504.0
60	37540.0
61	32576.0
62	29148.0
63	25720.0
64	21861.5
65	18003.0
66	15357.5
67	12712.0
68	10174.0
69	7636.0
70	7320.5
71	7005.0
72	5279.5
73	3554.0
74	2822.0
75	1619.0
76	1148.0
77	990.0
78	832.0
79	631.5
80	431.0
81	280.0
82	129.0
83	103.5
84	78.0
85	68.0
86	58.0
87	35.5
88	13.0
89	9.5
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1631920.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.10706903142403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69252088544677	20.259526921905373
2	8.251485583894379	4.143412363332728
3	2.9563119361147105	2.2267298357536465
4	1.4078467312560663	1.4138762026924292
5	0.8658002050311078	1.0868852757568552
6	0.5661203094401582	0.8528173013523112
7	0.4123339819628788	0.7246748424400764
8	0.3237913894980116	0.6503562214325828
9	0.26949232784697535	0.6089546230823849
>10	2.307280130303358	13.262264370175645
>50	1.0970689860586091	20.452226213467164
>100	0.8419030726161406	30.9174321400912
>500	0.0037708408737679123	0.5894450689853084
>1k	0.0040222302653524404	2.3567704118119086
>5k	2.5138939158452753E-4	0.45462820772043855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7203	0.44138193048678853	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4717	0.289046031668219	No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	3919	0.24014657581253981	TruSeq Adapter, Index 27 (100% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3623	0.22200843178587187	TruSeq Adapter, Index 27 (96% over 25bp)
GAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	3166	0.19400460806902298	TruSeq Adapter, Index 27 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2947	0.18058483258983285	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2879	0.1764179616647875	TruSeq Adapter, Index 27 (96% over 25bp)
AAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	2787	0.17078043041325555	TruSeq Adapter, Index 27 (100% over 22bp)
TAGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	1831	0.1121991274082063	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	1715	0.10509093583018776	No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1694	0.103804108044512	TruSeq Adapter, Index 27 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.127751360360802E-5	0.0	0.0	0.25957154762488355	0.0
2	6.127751360360802E-5	0.0	0.0	0.9140153929114172	0.0
3	6.127751360360802E-5	0.0	0.0	1.3193048678856807	0.0
4	6.127751360360802E-5	0.0	0.0	2.2164689445561057	0.0
5	6.127751360360802E-5	0.0	0.0	3.4892641796166477	0.0
6	6.127751360360802E-5	0.0	0.0	5.220599048972989	0.0
7	6.127751360360802E-5	0.0	0.0	6.087430756409628	0.0
8	6.127751360360802E-5	0.0	0.0	8.612186871905486	0.0
9	6.127751360360802E-5	0.0	0.0	9.61768959262709	0.0
10	6.127751360360802E-5	0.0	0.0	11.268076866513065	0.0
11	6.127751360360802E-5	0.0	0.0	12.500612775136036	0.0
12	6.127751360360802E-5	0.0	0.0	13.642764351193685	0.0
13	6.127751360360802E-5	0.0	0.0	14.09866905240453	0.0
14	6.127751360360802E-5	0.0	0.0	14.260380410804451	0.0
15	6.127751360360802E-5	0.0	0.0	14.490600029413207	0.0
16	6.127751360360802E-5	0.0	0.0	14.989215157605765	0.0
17	6.127751360360802E-5	0.0	0.0	15.539671062306976	0.0
18	6.127751360360802E-5	0.0	0.0	16.47133437913623	0.0
19	6.127751360360802E-5	0.0	0.0	16.765527721947155	0.0
20	6.127751360360802E-5	0.0	0.0	17.066829256336096	0.0
21	6.127751360360802E-5	0.0	0.0	17.42953085935585	0.0
22	6.127751360360802E-5	0.0	0.0	17.74762243247218	0.0
23	6.127751360360802E-5	0.0	0.0	18.161490759350947	0.0
24	6.127751360360802E-5	0.0	0.0	18.45231383891367	0.0
25	6.127751360360802E-5	0.0	0.0	18.66727535663513	0.0
26	6.127751360360802E-5	0.0	0.0	18.893757046914065	0.0
27	6.127751360360802E-5	0.0	0.0	19.11460120594147	0.0
28	6.127751360360802E-5	0.0	0.0	19.32374135987058	0.0
29	6.127751360360802E-5	0.0	0.0	19.544769351438795	0.0
30	6.127751360360802E-5	0.0	0.0	19.880876513554586	0.0
31	6.127751360360802E-5	0.0	0.0	20.155277219471543	0.0
32	6.127751360360802E-5	0.0	0.0	20.375569880876515	0.0
33	6.127751360360802E-5	0.0	0.0	20.60156135104662	0.0
34	6.127751360360802E-5	0.0	0.0	20.82510172067258	0.0
35	6.127751360360802E-5	0.0	0.0	21.133940389234766	0.0
36	6.127751360360802E-5	0.0	0.0	21.360789744595323	0.0
37	6.127751360360802E-5	0.0	0.0	21.591989803421736	0.0
38	6.127751360360802E-5	0.0	0.0	21.799475464483553	0.0
39	6.127751360360802E-5	0.0	0.0	22.013027599392128	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2123564E-7	45.0	1
TAATAGT	40	6.8175723E-9	45.0	35
CTCGTGA	20	7.034159E-4	45.0	32
GTCGACG	35	1.2123564E-7	45.0	1
CAACGAA	20	7.034159E-4	45.0	22
GCGGTAT	25	3.8915285E-5	45.0	28
GACGTAT	20	7.034159E-4	45.0	9
CGCGCGA	20	7.034159E-4	45.0	14
CGACAAC	20	7.034159E-4	45.0	36
TATGCGT	20	7.034159E-4	45.0	10
GTAATCG	55	1.8189894E-12	45.0	1
CGTTAGT	20	7.034159E-4	45.0	12
TGCGCAC	20	7.034159E-4	45.0	28
CGGATTA	25	3.8915285E-5	45.0	20
CGAATCG	25	3.8915285E-5	45.0	36
GCGATCG	20	7.034159E-4	45.0	9
CTACCGT	20	7.034159E-4	45.0	11
ATGCGTA	20	7.034159E-4	45.0	26
CGTAAAC	20	7.034159E-4	45.0	35
CGTCGAA	20	7.034159E-4	45.0	23
>>END_MODULE