FastQCFastQC Report
Thu 26 May 2016
SRR1546587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546587_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences887301
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78730.8872975461540108No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14950.16848848361491758No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9370.1056011432422594No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACA207.0324156E-445.00000413
TCTCGTC207.0324156E-445.00000441
GGACGTT207.0324156E-445.0000048
ATCGACT207.0324156E-445.00000433
AATCGAC207.0324156E-445.00000432
CAGGTCG253.890085E-545.02
ACGTTAG551.8189894E-1245.01
ACGCTAG351.2115015E-745.01
CGTCGTT253.890085E-545.024
ATCGCCG253.890085E-545.044
CATACGA1250.044.99999618
CGTTTTT53000.043.047171
AGGGTCG650.041.5384646
TGCGTAG650.041.5384641
TATTACG501.0804797E-940.51
GCGGCTA2000.040.524
TAGGGCG3700.040.1351365
GATTCGC451.9274012E-840.01
AGGGCGC1300.039.8076936
CGGCTAC2050.039.51219625
Produced by FastQC (version 0.11.5)