##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546587_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	887301
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.422246791111473	31.0	31.0	33.0	30.0	34.0
2	31.77382083419268	31.0	31.0	34.0	30.0	34.0
3	31.812523596840304	31.0	31.0	34.0	30.0	34.0
4	35.599905781690765	37.0	35.0	37.0	33.0	37.0
5	35.5364707128697	37.0	35.0	37.0	33.0	37.0
6	35.59448372085684	37.0	35.0	37.0	33.0	37.0
7	36.01866108569696	37.0	35.0	37.0	35.0	37.0
8	34.73553506645434	37.0	35.0	37.0	33.0	37.0
9	37.02935982265319	39.0	37.0	39.0	34.0	39.0
10	36.85219559089869	39.0	37.0	39.0	32.0	39.0
11	36.80706997963487	39.0	37.0	39.0	32.0	39.0
12	36.43475776540317	39.0	35.0	39.0	32.0	39.0
13	36.25943394631585	39.0	35.0	39.0	32.0	39.0
14	37.356499091063796	39.0	35.0	41.0	32.0	41.0
15	37.56630500810886	40.0	35.0	41.0	33.0	41.0
16	37.631761938733305	40.0	35.0	41.0	33.0	41.0
17	37.54445785590234	39.0	35.0	41.0	33.0	41.0
18	37.45974139553545	39.0	36.0	41.0	32.0	41.0
19	37.365422782122415	39.0	36.0	41.0	32.0	41.0
20	37.19468139898411	39.0	35.0	41.0	32.0	41.0
21	37.074961033516246	39.0	35.0	41.0	32.0	41.0
22	36.98564297797478	39.0	35.0	41.0	32.0	41.0
23	36.928918146153336	38.0	35.0	41.0	32.0	41.0
24	36.790261703751035	38.0	35.0	41.0	32.0	41.0
25	36.72004765012098	38.0	35.0	41.0	31.0	41.0
26	36.60742972227012	38.0	35.0	41.0	31.0	41.0
27	36.5054710859111	38.0	35.0	40.0	31.0	41.0
28	36.50231094070671	38.0	35.0	40.0	31.0	41.0
29	36.48233012247253	38.0	35.0	40.0	31.0	41.0
30	36.41916215579606	38.0	35.0	40.0	31.0	41.0
31	36.27858979083761	38.0	35.0	40.0	30.0	41.0
32	36.066775536148384	38.0	35.0	41.0	30.0	41.0
33	35.85778783073613	38.0	35.0	41.0	29.0	41.0
34	35.677422881299584	38.0	35.0	41.0	28.0	41.0
35	35.50506986918757	38.0	35.0	41.0	27.0	41.0
36	35.34954203815842	38.0	35.0	41.0	26.0	41.0
37	35.294416438164724	38.0	35.0	41.0	26.0	41.0
38	35.212651625547586	38.0	34.0	40.0	25.0	41.0
39	35.15518860003539	38.0	35.0	40.0	25.0	41.0
40	35.0366752657779	38.0	34.0	40.0	24.0	41.0
41	34.975520144798665	38.0	34.0	40.0	24.0	41.0
42	34.918805456096635	38.0	34.0	40.0	24.0	41.0
43	34.84106069980762	38.0	34.0	40.0	24.0	41.0
44	34.73759975476191	38.0	34.0	40.0	23.0	41.0
45	34.62521624567086	37.0	34.0	40.0	23.0	41.0
46	34.56970408012614	37.0	34.0	40.0	23.0	41.0
47	34.51452551050883	37.0	34.0	40.0	23.0	41.0
48	34.47401163753901	37.0	34.0	40.0	23.0	41.0
49	34.42472396627525	37.0	34.0	40.0	23.0	41.0
50	34.31496977913921	37.0	34.0	40.0	23.0	41.0
51	33.929450096415984	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	6.0
14	12.0
15	9.0
16	70.0
17	190.0
18	430.0
19	909.0
20	1486.0
21	2270.0
22	3458.0
23	5307.0
24	8026.0
25	11741.0
26	15665.0
27	17187.0
28	17508.0
29	18303.0
30	20573.0
31	24663.0
32	30462.0
33	39430.0
34	63706.0
35	106872.0
36	64479.0
37	80629.0
38	121897.0
39	231816.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.12156866722792	24.37064761563438	28.411102883914253	15.09668083322345
2	33.255118612511424	25.457313808955472	28.53642675935224	12.751140819180865
3	27.84094687146752	24.695340138239448	33.772868507980945	13.69084448231209
4	23.83881005431077	28.012478290906916	33.63345696668887	14.515254688093442
5	21.989155878332156	31.74075088386016	32.48446693962928	13.785626298178407
6	19.09171746678974	40.70163338032979	29.64236487956173	10.56428427331875
7	85.27354302542204	3.9063406893489354	9.151347738816929	1.6687685464120967
8	83.75106080123881	5.7112524385749595	8.312399061874155	2.225287698312072
9	81.43189289767508	4.688487897568018	10.880862300391863	2.998756904365035
10	38.70546747946864	29.319588279512814	17.968648744901675	14.006295496116875
11	33.31293439317661	28.244304920201824	24.695227436912614	13.74753324970895
12	30.68564106205222	25.003803669780606	28.618022519979124	15.692532748188045
13	21.2449890172557	34.14433208122159	29.815699520230453	14.794979381292256
14	17.17726002788231	37.07152364304785	30.528648113774242	15.222568215295599
15	15.210509173324496	26.847146571456587	42.98620197655587	14.956142278663046
16	17.612850656090774	23.740872601293134	41.426528314517846	17.219748428098246
17	17.340000743828757	23.830357454798314	31.36996351858051	27.45967828279242
18	20.79350750196382	25.442437233813553	34.298958301636084	19.46509696258654
19	24.207230691726934	27.202381153633322	29.51332186033826	19.077066294301485
20	26.062970739354512	25.75743744231101	28.456070713320507	19.72352110501397
21	21.589742376036995	28.786623704920878	30.858186793433116	18.76544712560901
22	21.545901559899065	25.897750594217744	29.24486729982272	23.31148054606047
23	19.152125377972073	30.14523819988933	28.20767698898119	22.49495943315741
24	19.111665601639128	27.341567292271733	36.202145607860245	17.3446214982289
25	17.820784604097145	27.554121994678244	34.537772413194624	20.08732098802999
26	17.065347610337415	32.54059220039198	30.21545112650611	20.178609062764497
27	18.303258984268023	31.4746630512081	30.500585483392896	19.72149248113098
28	16.739302671810353	29.632447162800446	35.72834922985548	17.899900935533715
29	16.800161388300026	26.933588489137282	35.14297853828633	21.123271584276363
30	18.65646494256177	29.57711081132558	32.6454044343464	19.121019811766242
31	24.754508334826625	28.578577055587672	27.750560407347674	18.916354202238022
32	24.224023189425008	28.647099462301966	28.230329955674566	18.898547392598452
33	22.833739621616566	29.20711235533376	27.440519057230862	20.518628965818813
34	19.39995559567723	29.736132383486552	29.04842888715329	21.815483133682932
35	19.420579938487617	28.66952702634168	30.03253687305661	21.877356162114097
36	24.893130966830874	28.865289231050117	27.0355831899209	19.205996612198113
37	20.03164653257463	32.212856741962426	28.625122703569588	19.13037402189336
38	20.39510831161015	32.149518596282434	26.56685837162361	20.888514720483805
39	20.234959726180858	31.95060075442268	27.376504703589877	20.437934815806585
40	22.325456637600993	29.004362668361694	27.043923088106514	21.626257605930796
41	18.884910532051695	28.085621452021353	28.06026365348399	24.96920436244296
42	21.585797829597848	28.768816895281308	26.26121237325327	23.384172901867572
43	21.645754935472855	28.410764779933757	27.45156378726047	22.491916497332923
44	20.496201401779103	30.918482003288624	27.888056026083596	20.697260568848677
45	19.05452602893494	32.93065149256002	25.900680828715394	22.114141649789644
46	22.020486847191652	31.166424922320612	26.8992145844533	19.913873646034435
47	21.400066042977524	29.560318313627505	27.672120283872104	21.367495359522866
48	22.22549056070037	27.40941349102503	28.822575428180514	21.542520520094083
49	20.90587072481604	26.89087468626768	29.88771566807656	22.315538920839714
50	20.058131344380318	30.538453129208694	28.225934603928092	21.1774809224829
51	19.505782141573153	31.319247921505777	26.38326791021311	22.79170202670796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1140.0
1	1258.5
2	1377.0
3	6833.0
4	12289.0
5	8577.5
6	4866.0
7	5137.0
8	5408.0
9	5915.0
10	6422.0
11	6847.5
12	7273.0
13	7224.5
14	7176.0
15	7124.0
16	7072.0
17	6703.0
18	6334.0
19	6243.0
20	6152.0
21	6215.5
22	6279.0
23	6486.5
24	6694.0
25	7072.5
26	9046.5
27	10642.0
28	12197.5
29	13753.0
30	14993.5
31	16234.0
32	18911.0
33	21588.0
34	23737.0
35	25886.0
36	28599.5
37	31313.0
38	35069.0
39	38825.0
40	47558.0
41	56291.0
42	63438.0
43	70585.0
44	73978.0
45	77371.0
46	79466.0
47	81561.0
48	81103.5
49	80646.0
50	76233.0
51	71820.0
52	64325.0
53	56830.0
54	49979.5
55	43129.0
56	37758.5
57	32388.0
58	27663.0
59	22938.0
60	20072.0
61	17206.0
62	14526.5
63	11847.0
64	9945.0
65	8043.0
66	6350.5
67	4658.0
68	3649.5
69	2641.0
70	2244.0
71	1847.0
72	1555.5
73	1264.0
74	1085.5
75	735.0
76	563.0
77	464.5
78	366.0
79	231.0
80	96.0
81	90.0
82	84.0
83	56.0
84	28.0
85	18.5
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	887301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.767969394594726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16399986657107	24.83111555520336
2	6.796719918394927	4.318359807024048
3	2.8214700691335897	2.6889712441200277
4	1.638383807466128	2.0819250660873414
5	1.1645035546646672	1.8496956642241955
6	0.903842347696574	1.7227941623498042
7	0.7391589902544574	1.64371061261002
8	0.5865874956743633	1.4907754887868112
9	0.5165369612176227	1.4768397337624561
>10	6.100689761321458	44.279324025914676
>50	0.5282849218287203	10.408191868529752
>100	0.038360936757086614	1.9328934786632161
>500	7.306845096587903E-4	0.18813620324925345
>1k	3.6534225482939516E-4	0.17351241447108817
>5k	3.6534225482939516E-4	0.9137546750039311
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7873	0.8872975461540108	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1495	0.16848848361491758	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	937	0.1056011432422594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.127013268327208E-4	0.0	0.0	0.08159576062688986	0.0
2	1.127013268327208E-4	0.0	0.0	0.24580159382216407	0.0
3	1.127013268327208E-4	0.0	0.0	0.3985118916805008	0.0
4	1.127013268327208E-4	0.0	0.0	0.5839055743203265	0.0
5	1.127013268327208E-4	0.0	0.0	0.9291097384089503	0.0
6	1.127013268327208E-4	0.0	0.0	1.4905877486895653	0.0
7	1.127013268327208E-4	0.0	0.0	1.809194399645667	0.0
8	1.127013268327208E-4	0.0	0.0	2.634618917368514	0.0
9	1.127013268327208E-4	0.0	0.0	3.057812399625381	0.0
10	1.127013268327208E-4	0.0	0.0	3.7006607678792203	0.0
11	1.127013268327208E-4	0.0	0.0	4.204999205455646	0.0
12	1.127013268327208E-4	0.0	0.0	4.6449851854105875	0.0
13	1.127013268327208E-4	0.0	0.0	4.854835055973114	0.0
14	1.127013268327208E-4	0.0	0.0	4.9391356484439894	0.0
15	1.127013268327208E-4	0.0	0.0	5.047216220876568	0.0
16	1.127013268327208E-4	0.0	0.0	5.260559832570909	0.0
17	1.127013268327208E-4	0.0	0.0	5.5242809373594755	0.0
18	1.127013268327208E-4	0.0	0.0	5.92831519405478	0.0
19	1.127013268327208E-4	0.0	0.0	6.089027286118239	0.0
20	1.127013268327208E-4	0.0	0.0	6.281183048368028	0.0
21	1.127013268327208E-4	0.0	0.0	6.522927394424214	0.0
22	1.127013268327208E-4	0.0	0.0	6.768841689573211	0.0
23	1.127013268327208E-4	0.0	0.0	7.050144201347682	0.0
24	1.127013268327208E-4	0.0	0.0	7.258078149354052	0.0
25	1.127013268327208E-4	0.0	0.0	7.44313372801338	0.0
26	1.127013268327208E-4	0.0	0.0	7.619849408487086	0.0
27	1.127013268327208E-4	0.0	0.0	7.807384416336734	0.0
28	1.127013268327208E-4	0.0	0.0	7.99987828256702	0.0
29	1.127013268327208E-4	0.0	0.0	8.21029165976371	0.0
30	1.127013268327208E-4	0.0	0.0	8.484381286620888	0.0
31	1.127013268327208E-4	0.0	0.0	8.709896641613161	0.0
32	1.127013268327208E-4	0.0	0.0	8.933270671395615	0.0
33	1.127013268327208E-4	0.0	0.0	9.150333426875434	0.0
34	1.127013268327208E-4	0.0	0.0	9.382160056170342	0.0
35	1.127013268327208E-4	0.0	0.0	9.631004585816989	0.0
36	1.127013268327208E-4	0.0	0.0	9.854491316926275	0.0
37	1.127013268327208E-4	0.0	0.0	10.081584490494206	0.0
38	1.127013268327208E-4	0.0	0.0	10.322652628589397	0.0
39	1.127013268327208E-4	0.0	0.0	10.554930063191634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGACA	20	7.0324156E-4	45.000004	13
TCTCGTC	20	7.0324156E-4	45.000004	41
GGACGTT	20	7.0324156E-4	45.000004	8
ATCGACT	20	7.0324156E-4	45.000004	33
AATCGAC	20	7.0324156E-4	45.000004	32
CAGGTCG	25	3.890085E-5	45.0	2
ACGTTAG	55	1.8189894E-12	45.0	1
ACGCTAG	35	1.2115015E-7	45.0	1
CGTCGTT	25	3.890085E-5	45.0	24
ATCGCCG	25	3.890085E-5	45.0	44
CATACGA	125	0.0	44.999996	18
CGTTTTT	5300	0.0	43.04717	1
AGGGTCG	65	0.0	41.538464	6
TGCGTAG	65	0.0	41.538464	1
TATTACG	50	1.0804797E-9	40.5	1
GCGGCTA	200	0.0	40.5	24
TAGGGCG	370	0.0	40.135136	5
GATTCGC	45	1.9274012E-8	40.0	1
AGGGCGC	130	0.0	39.807693	6
CGGCTAC	205	0.0	39.512196	25
>>END_MODULE