FastQCFastQC Report
Thu 26 May 2016
SRR1546586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546586_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2018525
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224971.1145266964739104No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT207.034556E-445.042
CGTTTTT151600.042.4175451
TAGTACG1550.042.0967751
TACGTAG700.041.7857171
TAACGCG403.4599543E-739.3751
TACGGGA4750.039.315794
TGATTCG3400.039.0441215
AGTACGG2200.038.8636362
CGTTCGG6150.038.41463545
ACGGGAT4950.038.1818165
ACGTTAG1150.037.1739161
ATAACGG850.037.0588262
ACTACGG1100.036.818182
CAATGCG552.752131E-936.818181
GGCGATA8750.036.7714278
GGGCGAT46500.036.5322577
TAGGGAC13750.036.1636355
CTACGGG3050.036.147543
TGGGCGA13800.036.0326086
CTCGTTA250.002107485236.041
Produced by FastQC (version 0.11.5)