FastQCFastQC Report
Thu 26 May 2016
SRR1546585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546585_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1413308
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63180.44703631480186906No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41110.2908778553577847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA850.045.0000046
TGTAACG650.045.0000041
ACAACGC207.033837E-445.039
ATTAGCG750.045.01
GATCGAC253.8912614E-545.010
CCGTAAG207.033837E-445.01
CTACGAA1500.045.011
GCGTAAT207.033837E-445.01
GCGTAAG453.8562575E-1045.01
TGCGTTA207.033837E-445.022
ACGTTAG551.8189894E-1244.9999961
CGATCTA302.165858E-644.99999610
CGAATAT1500.043.514
GCAAGCG700.041.7857171
GCACCGT700.041.7857179
CGTTAGG1400.041.7857172
CGTAAGG1400.041.7857172
CGGTCTA1750.041.14285731
TCTAGCG556.184564E-1140.909091
TAACGTG501.0822987E-940.51
Produced by FastQC (version 0.11.5)