##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546585_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1413308
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42263469816912	31.0	31.0	33.0	30.0	34.0
2	31.767468237638223	31.0	31.0	34.0	30.0	34.0
3	31.864622573423485	31.0	31.0	34.0	30.0	34.0
4	35.61202724388456	37.0	35.0	37.0	33.0	37.0
5	35.54026793876494	37.0	35.0	37.0	33.0	37.0
6	35.58653669263883	37.0	35.0	37.0	33.0	37.0
7	35.97031644906843	37.0	35.0	37.0	35.0	37.0
8	34.639794722735594	37.0	35.0	37.0	33.0	37.0
9	36.91141562914807	39.0	37.0	39.0	33.0	39.0
10	36.86017838999001	39.0	37.0	39.0	32.0	39.0
11	36.730349647776706	39.0	35.0	39.0	32.0	39.0
12	36.0651146105449	37.0	35.0	39.0	32.0	39.0
13	35.73918494765472	37.0	35.0	39.0	31.0	39.0
14	36.731435752150276	39.0	35.0	41.0	31.0	41.0
15	37.05751683284889	39.0	35.0	41.0	32.0	41.0
16	37.186667732723514	39.0	35.0	41.0	32.0	41.0
17	37.10922530686871	38.0	35.0	41.0	32.0	41.0
18	37.06877340254212	38.0	35.0	41.0	32.0	41.0
19	37.00366232979648	38.0	35.0	41.0	32.0	41.0
20	36.843317946265074	38.0	35.0	41.0	32.0	41.0
21	36.70317085872294	38.0	35.0	41.0	32.0	41.0
22	36.606291056160444	38.0	35.0	41.0	31.0	41.0
23	36.577172137991155	38.0	35.0	40.0	31.0	41.0
24	36.437669637474634	38.0	35.0	40.0	31.0	41.0
25	36.34177122042754	38.0	35.0	40.0	31.0	41.0
26	36.22302781842316	37.0	35.0	40.0	31.0	41.0
27	36.099487160618914	37.0	35.0	40.0	31.0	41.0
28	36.14569930970461	37.0	35.0	40.0	31.0	41.0
29	36.150049387677704	37.0	35.0	40.0	31.0	41.0
30	36.12180713616565	37.0	35.0	40.0	31.0	41.0
31	35.97674958324725	37.0	35.0	40.0	30.0	41.0
32	35.82941510272354	37.0	35.0	40.0	30.0	41.0
33	35.69291831646039	37.0	35.0	40.0	30.0	41.0
34	35.56550518358348	37.0	35.0	40.0	29.0	41.0
35	35.44928989293204	37.0	35.0	40.0	29.0	41.0
36	35.26101812202294	37.0	34.0	40.0	27.0	41.0
37	35.19310228202204	37.0	34.0	40.0	27.0	41.0
38	35.12219841676407	37.0	34.0	40.0	27.0	41.0
39	35.03158759449462	36.0	34.0	40.0	27.0	41.0
40	34.87372603848559	36.0	34.0	40.0	26.0	41.0
41	34.82220860562595	36.0	34.0	40.0	26.0	41.0
42	34.76009900177456	36.0	34.0	40.0	26.0	41.0
43	34.66508078918396	36.0	34.0	40.0	26.0	41.0
44	34.555010655851376	36.0	34.0	40.0	26.0	41.0
45	34.44558440198456	35.0	34.0	40.0	25.0	41.0
46	34.381945053732096	35.0	34.0	40.0	25.0	41.0
47	34.313487222884184	35.0	34.0	40.0	24.0	41.0
48	34.27379523783917	35.0	34.0	40.0	24.0	41.0
49	34.22879443122094	35.0	34.0	40.0	24.0	41.0
50	34.09809468282922	35.0	34.0	40.0	24.0	41.0
51	33.722087471379204	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	7.0
13	9.0
14	12.0
15	47.0
16	116.0
17	305.0
18	704.0
19	1339.0
20	2341.0
21	3629.0
22	5491.0
23	7954.0
24	11373.0
25	15819.0
26	20922.0
27	24805.0
28	27041.0
29	30190.0
30	35873.0
31	43786.0
32	54566.0
33	71086.0
34	127135.0
35	218649.0
36	100374.0
37	126266.0
38	180898.0
39	302279.0
40	287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.176626750856855	24.227132373127443	28.40583934995061	13.19040152606509
2	31.42761521197078	26.114265255697976	29.470646171959686	12.987473360371554
3	29.50000990583794	25.869025010825663	31.298697806847482	13.332267276488919
4	26.15028005218962	28.140433649282393	30.49377771865722	15.21550857987077
5	23.327045484777557	32.93379786996182	28.515440371100993	15.223716274159631
6	21.23797502030697	41.406614835548936	26.461960167210545	10.89344997693355
7	84.92508356281859	5.26127355112969	7.57294234519298	2.240700540858751
8	82.46645458739354	7.329187976010891	6.7886830046953675	3.4156744319001944
9	79.5514495071138	6.517829093162991	10.052090556340161	3.8786308433830414
10	49.167202053621715	23.08215901983149	14.803637989737553	12.947000936809244
11	43.9043011148313	21.149742306701725	20.706880595029535	14.239075983437438
12	38.71109482151095	21.20224324775633	24.845115148290393	15.241546782442327
13	22.45632233030592	36.80924469400866	25.61083642065282	15.12359655503259
14	16.7721402553442	40.14213462316778	27.561295910021027	15.524429211466998
15	15.159469839553728	23.371621755484295	46.21674822473233	15.252160180229646
16	16.628859385215396	18.267992539488915	44.894955664299644	20.208192410996045
17	16.601759842865107	20.05493494694716	29.195122365400888	34.14818284478684
18	21.004904804897446	23.081805239905243	34.273350182691956	21.639939772505357
19	28.11779173400278	24.555227876726093	26.617481822787393	20.709498566483738
20	29.628432019064494	23.370489659720317	24.685914181480612	22.31516413973458
21	22.557361877241195	28.13010327543607	27.8646268187826	21.447908028540134
22	22.383372909514414	25.075850416186707	25.62173284238114	26.919043831917744
23	19.77679316893416	29.517911170105883	24.627894273576604	26.077401387383357
24	20.286023994769717	24.430768098673468	36.31105180187192	18.972156104684895
25	19.030671304485647	24.961013452127915	33.07658344819388	22.931731795192555
26	17.576352783681973	31.58752373863305	27.79655956097326	23.039563916711714
27	19.144871464677195	31.238413707415507	28.437042739445328	21.179672088461963
28	17.171770060029377	28.493010723777125	35.544622969657006	18.790596246536495
29	17.67781686652874	24.924857143665783	34.12087103448081	23.27645495532467
30	20.5829868648589	28.595394634432125	30.289151409317718	20.53246709139126
31	28.377112419939603	26.75736640562425	23.75349180787203	21.112029366564116
32	28.951297240233554	26.031693020912638	26.120562538385123	18.89644720046869
33	27.39445329680438	26.616137459067662	24.66390907006824	21.325500174059727
34	21.310287637231234	26.680879185570305	28.738321724634687	23.27051145256377
35	22.96774659168419	24.392842890580113	29.056440634313258	23.58296988342244
36	29.745108638739755	24.720372346296774	26.014782340438174	19.519736674525298
37	22.840385818236363	29.58866715535467	28.706835311198976	18.86411171520999
38	22.445638176533354	31.69231335278651	24.100337647561606	21.761710823118527
39	22.555663733595225	29.410928120409707	26.614368559436443	21.41903958655863
40	24.922663708123068	24.627115957738866	26.52705567363943	23.92316466049863
41	19.167088844045317	23.48900593501204	27.843541535178463	29.50036368576418
42	23.434099290458978	25.31861420157531	25.53795775584657	25.70932875211914
43	22.771752512545035	24.938796072759793	27.37492464487571	24.91452676981946
44	21.499489141786505	28.60657408010144	27.70691172766304	22.187025050449016
45	19.390960781372495	32.67412340409875	24.484259623521552	23.450656191007198
46	23.815757074890964	29.8273978495841	26.29073068290847	20.06611439261647
47	23.610069425772725	26.72354504467533	27.433156820735466	22.23322870881648
48	23.887149864006997	23.98408556379784	29.165192583640646	22.963571988554513
49	22.325777537521898	22.437005946333	31.594528581172682	23.64268793497242
50	21.092500714635452	28.705561703464493	28.006421813221184	22.195515768678874
51	20.781598915452257	29.6835509315733	25.1728568719628	24.36199328101164
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1799.0
1	2385.5
2	2972.0
3	7232.5
4	11493.0
5	8350.5
6	5208.0
7	5620.0
8	6032.0
9	6419.5
10	6807.0
11	7149.5
12	7492.0
13	7453.0
14	7414.0
15	7440.0
16	7466.0
17	7119.5
18	6773.0
19	6741.0
20	6709.0
21	6131.0
22	5553.0
23	6054.0
24	6555.0
25	7063.0
26	8925.5
27	10280.0
28	12537.0
29	14794.0
30	16696.0
31	18598.0
32	21026.0
33	23454.0
34	26016.0
35	28578.0
36	29447.5
37	30317.0
38	38964.0
39	47611.0
40	65419.0
41	83227.0
42	98821.5
43	114416.0
44	118628.0
45	122840.0
46	121546.0
47	120252.0
48	121838.5
49	123425.0
50	122655.5
51	121886.0
52	114024.0
53	106162.0
54	90445.5
55	74729.0
56	68385.0
57	62041.0
58	58055.5
59	54070.0
60	50524.5
61	46979.0
62	41137.5
63	35296.0
64	31409.5
65	27523.0
66	23637.5
67	19752.0
68	16255.5
69	12759.0
70	10632.0
71	8505.0
72	7923.5
73	7342.0
74	5652.5
75	3203.5
76	2444.0
77	1689.0
78	934.0
79	825.0
80	716.0
81	500.5
82	285.0
83	242.0
84	199.0
85	120.0
86	41.0
87	30.0
88	19.0
89	16.0
90	13.0
91	9.0
92	5.0
93	5.5
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1413308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.953093370266945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.28339509260437	18.65705357012846
2	7.263289655945101	3.3342993129640397
3	2.3326714520567324	1.6062607692364308
4	1.1532739746930492	1.0588482089051374
5	0.7229830138304644	0.8297348310783823
6	0.504538362922135	0.6948429691820036
7	0.37243867152388	0.5984033721530059
8	0.3138398429028765	0.5762876173967707
9	0.249633589525405	0.5156876779858363
>10	3.314705440745061	19.14475241994688
>50	1.7868462853190457	29.730290993424223
>100	0.6963350677320406	21.51243155377662
>500	0.004139165926161405	0.6296749227725068
>1k	0.0015919868946774633	0.6496994038356064
>5k	3.183973789354927E-4	0.4617323772141015
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6318	0.44703631480186906	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4111	0.2908778553577847	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10379903035997814	0.0
2	0.0	0.0	0.0	0.32774172367240545	0.0
3	0.0	0.0	0.0	0.4812114556770357	0.0
4	0.0	0.0	0.0	0.7849669003501006	0.0
5	0.0	0.0	0.0	1.2711312749945518	0.0
6	0.0	0.0	0.0	1.9687852895476428	0.0
7	0.0	0.0	0.0	2.346905274717188	0.0
8	0.0	0.0	0.0	3.446453285483419	0.0
9	0.0	0.0	0.0	3.9823591177577713	0.0
10	0.0	0.0	0.0	4.841690558604352	0.0
11	0.0	0.0	0.0	5.478494425843482	0.0
12	0.0	0.0	0.0	6.061169964367286	0.0
13	0.0	0.0	0.0	6.306905501136341	0.0
14	0.0	0.0	0.0	6.396270310505566	0.0
15	7.07559852487922E-5	0.0	0.0	6.520305552646699	0.0
16	7.07559852487922E-5	0.0	0.0	6.786418813167406	0.0
17	7.07559852487922E-5	0.0	0.0	7.065692686944389	0.0
18	7.07559852487922E-5	0.0	0.0	7.53721057264234	0.0
19	7.07559852487922E-5	0.0	0.0	7.706954181254193	0.0
20	7.07559852487922E-5	0.0	0.0	7.900896336821131	0.0
21	7.07559852487922E-5	0.0	0.0	8.129155145233735	0.0
22	7.07559852487922E-5	0.0	0.0	8.344607120316308	0.0
23	7.07559852487922E-5	0.0	0.0	8.578809431489809	0.0
24	7.07559852487922E-5	0.0	0.0	8.764826916708884	0.0
25	7.07559852487922E-5	0.0	0.0	8.915324897333065	0.0
26	7.07559852487922E-5	0.0	0.0	9.059313327314358	0.0
27	7.07559852487922E-5	0.0	0.0	9.204504609044879	0.0
28	7.07559852487922E-5	0.0	0.0	9.338162665179848	0.0
29	7.07559852487922E-5	0.0	0.0	9.495240952432166	0.0
30	7.07559852487922E-5	0.0	0.0	9.718617597862604	0.0
31	7.07559852487922E-5	0.0	0.0	9.892323541648388	0.0
32	7.07559852487922E-5	0.0	0.0	10.06086429851101	0.0
33	7.07559852487922E-5	0.0	0.0	10.22898051946214	0.0
34	7.07559852487922E-5	0.0	0.0	10.40537519068738	0.0
35	7.07559852487922E-5	0.0	0.0	10.612336447540097	0.0
36	7.07559852487922E-5	0.0	0.0	10.789792458544069	0.0
37	7.07559852487922E-5	0.0	0.0	10.968805101223513	0.0
38	7.07559852487922E-5	0.0	0.0	11.1335250348827	0.0
39	7.07559852487922E-5	0.0	0.0	11.314660357119608	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATA	85	0.0	45.000004	6
TGTAACG	65	0.0	45.000004	1
ACAACGC	20	7.033837E-4	45.0	39
ATTAGCG	75	0.0	45.0	1
GATCGAC	25	3.8912614E-5	45.0	10
CCGTAAG	20	7.033837E-4	45.0	1
CTACGAA	150	0.0	45.0	11
GCGTAAT	20	7.033837E-4	45.0	1
GCGTAAG	45	3.8562575E-10	45.0	1
TGCGTTA	20	7.033837E-4	45.0	22
ACGTTAG	55	1.8189894E-12	44.999996	1
CGATCTA	30	2.165858E-6	44.999996	10
CGAATAT	150	0.0	43.5	14
GCAAGCG	70	0.0	41.785717	1
GCACCGT	70	0.0	41.785717	9
CGTTAGG	140	0.0	41.785717	2
CGTAAGG	140	0.0	41.785717	2
CGGTCTA	175	0.0	41.142857	31
TCTAGCG	55	6.184564E-11	40.90909	1
TAACGTG	50	1.0822987E-9	40.5	1
>>END_MODULE