FastQCFastQC Report
Thu 26 May 2016
SRR1546584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546584_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2190578
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT575162.6256084010704024No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT63390.28937568075640313RNA PCR Primer, Index 41 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC49580.22633295869857178Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT46870.21396179455833117RNA PCR Primer, Index 41 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG24100.11001662574900324RNA PCR Primer, Index 41 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTC207.034688E-445.038
ATACTCG302.1664073E-644.99999644
CGTTTTT357150.044.2566151
ACGTTCG850.042.35294313
CGTTCGA850.042.35294314
CGGTCTA1450.041.89655331
AATACGG650.041.538462
TGTTACG1200.041.2499961
TATTACG556.184564E-1140.9090921
ACGGGTA2350.040.2127655
TTACCGG451.9297659E-840.02
GTTTTTT386900.039.6555982
CTCACGA1600.039.37524
TAGCGCG800.039.3751
TACGGGA6400.039.3754
TACGAAC403.4601362E-739.37530
CGACGGT1550.039.19354628
CGTAAGG2600.038.9423072
GCGATAC1050.038.571439
ACGCCGA1400.038.57142612
Produced by FastQC (version 0.11.5)