FastQCFastQC Report
Thu 26 May 2016
SRR1546583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546583_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences877143
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45080.5139412843743837No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12840.14638434097974903No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.032372E-445.01
CGTATGT207.032372E-445.018
CGTACTA207.032372E-445.06
ATACCGG207.032372E-445.02
TATGACG603.6379788E-1241.2500041
ATAACGC1050.040.71428711
CGTTTTT29150.040.3687821
TAGCATA5150.039.75728230
TGGGATC4750.039.315796
GCGATGA2600.038.942319
CGGGTAT356.246599E-638.5714266
CGGTCTA1350.038.33333231
TAGGGAT7700.038.279225
GTTGATC5600.038.1696416
GCGATAT1300.038.0769279
GTAGCAT5500.038.04545629
ACGGGAT2250.038.05
CGTTCGG3150.037.85714345
CATATGC5300.037.7830233
GGTACCT5600.037.7678578
Produced by FastQC (version 0.11.5)