##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546583_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	877143
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38380172902252	31.0	31.0	33.0	30.0	34.0
2	31.753255740512095	31.0	31.0	34.0	30.0	34.0
3	31.872845134715774	31.0	31.0	34.0	30.0	34.0
4	35.637233609571076	37.0	35.0	37.0	33.0	37.0
5	35.55942759618443	37.0	35.0	37.0	33.0	37.0
6	35.60300315912001	37.0	35.0	37.0	33.0	37.0
7	35.954698378713616	37.0	35.0	37.0	35.0	37.0
8	34.62166260233508	37.0	35.0	37.0	33.0	37.0
9	36.90247428298464	39.0	37.0	39.0	33.0	39.0
10	36.841963055054876	39.0	37.0	39.0	32.0	39.0
11	36.77791648568135	39.0	37.0	39.0	32.0	39.0
12	36.36641345823885	38.0	35.0	39.0	32.0	39.0
13	36.22650354617206	39.0	35.0	39.0	32.0	39.0
14	37.29194213486284	39.0	35.0	41.0	32.0	41.0
15	37.494466694712266	39.0	35.0	41.0	32.0	41.0
16	37.55811880160932	39.0	35.0	41.0	33.0	41.0
17	37.46246507125976	39.0	35.0	41.0	32.0	41.0
18	37.40289667705266	39.0	35.0	41.0	32.0	41.0
19	37.353466880542854	39.0	35.0	41.0	32.0	41.0
20	37.21362537237372	39.0	35.0	41.0	32.0	41.0
21	37.10082392494724	39.0	35.0	41.0	32.0	41.0
22	36.98171107789722	39.0	35.0	41.0	32.0	41.0
23	36.912933238935956	39.0	35.0	41.0	31.0	41.0
24	36.76863863702954	38.0	35.0	41.0	31.0	41.0
25	36.66098914316138	38.0	35.0	41.0	31.0	41.0
26	36.52137906817931	38.0	35.0	41.0	31.0	41.0
27	36.4109899982101	38.0	35.0	40.0	31.0	41.0
28	36.42705123337928	38.0	35.0	40.0	31.0	41.0
29	36.37036720352326	38.0	35.0	40.0	30.0	41.0
30	36.31374473717512	38.0	35.0	40.0	30.0	41.0
31	36.215102896563046	38.0	35.0	40.0	30.0	41.0
32	36.11864200022117	38.0	35.0	40.0	30.0	41.0
33	35.998600000228016	38.0	35.0	41.0	30.0	41.0
34	35.86436077127675	38.0	35.0	41.0	29.0	41.0
35	35.75441632664229	38.0	35.0	41.0	29.0	41.0
36	35.60890527542259	38.0	35.0	40.0	28.0	41.0
37	35.555981179807624	38.0	35.0	40.0	28.0	41.0
38	35.44703657214388	38.0	34.0	40.0	27.0	41.0
39	35.37343397826808	38.0	34.0	40.0	27.0	41.0
40	35.214447359210524	38.0	34.0	40.0	27.0	41.0
41	35.1391688698422	37.0	34.0	40.0	26.0	41.0
42	35.07069428816054	37.0	34.0	40.0	26.0	41.0
43	34.97297247997191	37.0	34.0	40.0	26.0	41.0
44	34.84869171845412	37.0	34.0	40.0	26.0	41.0
45	34.71580346648152	36.0	34.0	40.0	26.0	41.0
46	34.64196943941866	36.0	34.0	40.0	25.0	41.0
47	34.56242368690168	36.0	34.0	40.0	25.0	41.0
48	34.48746555578737	36.0	34.0	40.0	25.0	41.0
49	34.41313332033659	36.0	34.0	40.0	25.0	41.0
50	34.30057356668183	35.0	34.0	40.0	24.0	41.0
51	33.94132883691713	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	3.0
13	5.0
14	8.0
15	31.0
16	75.0
17	203.0
18	430.0
19	814.0
20	1384.0
21	2255.0
22	3277.0
23	4833.0
24	7012.0
25	9457.0
26	12472.0
27	14562.0
28	16166.0
29	18074.0
30	21203.0
31	25669.0
32	31800.0
33	41378.0
34	67591.0
35	103085.0
36	71213.0
37	91488.0
38	129332.0
39	203141.0
40	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.93535261639209	23.819833254098818	27.0124711706073	14.232342958901798
2	32.41136279945231	25.605745015350973	28.434930222324066	13.547961962872645
3	29.662894191711043	25.669702659657545	30.113105844771038	14.554297303860375
4	26.25147780920557	28.233138724244505	29.60771504760341	15.90766841894651
5	22.98382361826977	32.63960380462479	28.910109298027802	15.466463279077642
6	21.55270007285015	41.173674075948846	26.411428923220043	10.862196927980957
7	85.81884595784268	4.8028656672857215	7.156985805051172	2.2213025698204283
8	82.81785296126174	6.9579304628777745	6.9708131969359615	3.2534033789245314
9	79.66488930539262	6.165927334539522	9.88903747735546	4.280145882712397
10	43.510351219812506	25.92918144475872	15.823987650816344	14.73647968461243
11	36.82808846448071	23.220501104152916	22.980517429883154	16.970893001483226
12	32.30716086202592	21.926983399514103	27.818839117452914	17.947016621007066
13	24.011934200010717	29.579783456061325	28.937014831105078	17.47126751282288
14	20.040517908710438	33.13040177029287	29.092405685275946	17.736674635720743
15	18.19828693838975	23.33325352878607	39.77629645337191	18.692163079452268
16	20.951315805974623	20.223270321942945	38.166638735075125	20.658775137007307
17	21.18753726587341	21.15139720661283	28.59225918692847	29.068806340585283
18	23.8142469357904	23.349556457726962	31.17769850526083	21.658498101221806
19	27.65375771111438	25.051331424864586	25.450012141691836	21.844898722329198
20	28.945337305319658	23.956527042910906	24.361934143007467	22.73620150876197
21	25.889165164631077	25.62535413267848	27.243106312197668	21.24237439049277
22	25.193269512496823	23.144344764764696	26.224002243647842	25.438383479090636
23	22.75717870404256	27.59436032665141	25.055777678212106	24.592683291093927
24	22.967178669840607	24.89046825888139	31.66644435399929	20.47590871727871
25	22.90652721392065	24.716494345847824	29.031412209867717	23.345566230363808
26	20.52173932870695	28.107047539568804	26.867568914076724	24.503644217647523
27	21.67651112760405	26.999474430053024	28.04240585628569	23.281608586057235
28	20.46849829503285	25.803432279571293	32.422421429573056	21.305647995822802
29	21.33506167181406	24.811347750594827	30.378284954676715	23.475305622914394
30	22.69139695579854	25.9976993489089	29.653431652535563	21.657472042756996
31	27.649767483751226	25.49128249327647	24.552324991478013	22.306625031494296
32	27.061835983414333	25.31901867768425	25.16157570658376	22.45756963231765
33	26.569897952785347	25.320386755637337	24.993188111858615	23.1165271797187
34	21.573335248642465	26.723350696522687	27.372161665771717	24.331152389063128
35	22.783628211135472	25.632764554924343	28.487601223517718	23.096006010422474
36	27.23159165609256	26.34747127891347	25.072080607152998	21.348856457840967
37	22.452439339993592	29.008154884665327	27.00369267040836	21.535713104932718
38	22.822960452286573	30.266216569020106	24.769507366529744	22.14131561216358
39	21.796673974483067	28.205321139198514	26.280549465708557	23.717455420609866
40	23.613025470191292	25.379214107619852	27.520712130177177	23.48704829201168
41	20.67006178012023	24.444588852672826	28.39799211759086	26.487357249616082
42	23.857569404304655	25.957341049292985	25.421510517669297	24.76357902873306
43	23.685761614696805	25.230093610733938	27.099686140116262	23.98445863445299
44	22.323840012403906	27.5366730396298	27.475337544733296	22.664149403232997
45	21.35056655528232	28.795646775953294	26.083888259953053	23.769898408811336
46	24.151592157721147	27.21551674014385	26.90325294735294	21.72963815478206
47	23.686445653673346	26.689262754191734	27.17994671336373	22.44434487877119
48	23.393562965217757	25.40862778361111	27.9508586399253	23.24695061124583
49	22.477178749645155	24.44094064479794	28.567633783773	24.514246821783907
50	21.576755443525172	26.884099856009797	28.58325267373735	22.955892026727685
51	21.70683685556403	28.075581746647927	26.197324723562748	24.020256674225298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	938.0
1	1248.5
2	1559.0
3	4621.0
4	7683.0
5	5487.5
6	3292.0
7	3424.5
8	3557.0
9	3903.5
10	4250.0
11	4321.0
12	4392.0
13	4453.5
14	4515.0
15	4305.0
16	4095.0
17	4158.0
18	4221.0
19	4012.0
20	3803.0
21	3817.0
22	3831.0
23	4042.5
24	4254.0
25	4518.0
26	5759.5
27	6737.0
28	7266.0
29	7795.0
30	8856.0
31	9917.0
32	12038.0
33	14159.0
34	15720.0
35	17281.0
36	19351.0
37	21421.0
38	24947.5
39	28474.0
40	35050.0
41	41626.0
42	49078.5
43	56531.0
44	61386.0
45	66241.0
46	70515.5
47	74790.0
48	72823.0
49	70856.0
50	66772.0
51	62688.0
52	58845.0
53	55002.0
54	52007.0
55	49012.0
56	47519.0
57	46026.0
58	43855.0
59	41684.0
60	39966.0
61	38248.0
62	34753.0
63	31258.0
64	28462.5
65	25667.0
66	22484.0
67	19301.0
68	16389.0
69	13477.0
70	11419.0
71	9361.0
72	7822.0
73	6283.0
74	5187.5
75	3072.5
76	2053.0
77	1542.0
78	1031.0
79	802.0
80	573.0
81	413.5
82	254.0
83	163.5
84	73.0
85	52.0
86	31.0
87	24.5
88	18.0
89	12.5
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	877143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.10229756231564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.29728201102193	21.722312198319667
2	6.1687173598949	3.4671026165117724
3	2.4824538786909747	2.0928797275109523
4	1.5286702836601964	1.718365887443532
5	1.079510578883338	1.5168363754723588
6	0.8808177702244872	1.4851801846214348
7	0.6846455289399958	1.3468078665286523
8	0.587097464246885	1.3199030110677545
9	0.5397159897608115	1.3650533409058223
>10	8.31679841986793	54.27677560416506
>50	0.39378475397823953	6.759100379438074
>100	0.03800043747994638	1.9575421157819899
>500	0.0016703489002174233	0.2924401985809435
>1k	8.351744501087116E-4	0.6797004936520163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4508	0.5139412843743837	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1284	0.14638434097974903	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1400649609014721E-4	0.0	0.0	0.042068397057264326	0.0
2	2.2801299218029442E-4	0.0	0.0	0.1228990027851787	0.0
3	2.2801299218029442E-4	0.0	0.0	0.20156348508738028	0.0
4	2.2801299218029442E-4	0.0	0.0	0.34532567665705594	0.0
5	2.2801299218029442E-4	0.0	0.0	0.6038924097895098	0.0
6	2.2801299218029442E-4	0.0	0.0	1.089560083133537	0.0
7	2.2801299218029442E-4	0.0	0.0	1.3852929339913789	0.0
8	2.2801299218029442E-4	0.0	0.0	2.2753416489671583	0.0
9	2.2801299218029442E-4	0.0	0.0	2.765569582154791	0.0
10	2.2801299218029442E-4	0.0	0.0	3.432507584282152	0.0
11	2.2801299218029442E-4	0.0	0.0	3.8343804829999213	0.0
12	2.2801299218029442E-4	0.0	0.0	4.22086250474552	0.0
13	2.2801299218029442E-4	0.0	0.0	4.401448794552314	0.0
14	2.2801299218029442E-4	0.0	0.0	4.474070932561737	0.0
15	2.2801299218029442E-4	0.0	0.0	4.546921083563341	0.0
16	2.2801299218029442E-4	0.0	0.0	4.694559496000082	0.0
17	2.2801299218029442E-4	0.0	0.0	4.842881947413364	0.0
18	2.2801299218029442E-4	0.0	0.0	5.1008786480653665	0.0
19	2.2801299218029442E-4	0.0	0.0	5.207360715413564	0.0
20	2.2801299218029442E-4	0.0	0.0	5.338126166428963	0.0
21	2.2801299218029442E-4	0.0	0.0	5.489184773748408	0.0
22	2.2801299218029442E-4	0.0	0.0	5.639901361579583	0.0
23	2.2801299218029442E-4	0.0	0.0	5.821741722843368	0.0
24	2.2801299218029442E-4	0.0	0.0	5.960031602600716	0.0
25	2.2801299218029442E-4	0.0	0.0	6.0733540597143225	0.0
26	2.2801299218029442E-4	0.0	0.0	6.190552737694994	0.0
27	2.2801299218029442E-4	0.0	0.0	6.310031545597468	0.0
28	2.2801299218029442E-4	0.0	0.0	6.429396347003852	0.0
29	2.2801299218029442E-4	0.0	0.0	6.558451700577899	0.0
30	2.2801299218029442E-4	0.0	0.0	6.747930497079723	0.0
31	2.2801299218029442E-4	0.0	0.0	6.898305065422628	0.0
32	2.2801299218029442E-4	0.0	0.0	7.03933110108614	0.0
33	2.2801299218029442E-4	0.0	0.0	7.192441825335208	0.0
34	2.2801299218029442E-4	0.0	0.0	7.345894569072546	0.0
35	3.4201948827044165E-4	0.0	0.0	7.520324508090471	0.0
36	3.4201948827044165E-4	0.0	0.0	7.663630673675786	0.0
37	3.4201948827044165E-4	0.0	0.0	7.810015014655535	0.0
38	3.4201948827044165E-4	0.0	0.0	7.9819368107594775	0.0
39	4.5602598436058885E-4	0.0	0.0	8.203565439158723	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.032372E-4	45.0	1
CGTATGT	20	7.032372E-4	45.0	18
CGTACTA	20	7.032372E-4	45.0	6
ATACCGG	20	7.032372E-4	45.0	2
TATGACG	60	3.6379788E-12	41.250004	1
ATAACGC	105	0.0	40.714287	11
CGTTTTT	2915	0.0	40.368782	1
TAGCATA	515	0.0	39.757282	30
TGGGATC	475	0.0	39.31579	6
GCGATGA	260	0.0	38.94231	9
CGGGTAT	35	6.246599E-6	38.571426	6
CGGTCTA	135	0.0	38.333332	31
TAGGGAT	770	0.0	38.27922	5
GTTGATC	560	0.0	38.16964	16
GCGATAT	130	0.0	38.076927	9
GTAGCAT	550	0.0	38.045456	29
ACGGGAT	225	0.0	38.0	5
CGTTCGG	315	0.0	37.857143	45
CATATGC	530	0.0	37.78302	33
GGTACCT	560	0.0	37.767857	8
>>END_MODULE