FastQCFastQC Report
Thu 26 May 2016
SRR1546582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546582_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2928545
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304641.040243533905062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT183600.042.1323551
TACGGGA6850.041.3868644
CGTTCGG7950.039.90566345
GCGATAT2650.039.0566029
GGGCGAT61050.038.1449627
CGGTCTA2500.037.80000331
AGGGCGA30700.037.524436
ACGGGAT6100.037.25415
GGCGATT16200.037.222228
AGGGTAC12400.036.4717756
TTACGGG7150.036.1888123
GGCGATA12150.036.111118
TACGAAC250.002107639836.034
GCGTAAG1500.036.01
GTACGGG9450.035.952383
CTCACGA2650.035.66037824
CGTAAGG3300.035.4545442
TAAGGGA20900.035.203354
TAGCGGG15550.035.1607743
TAGGGAC16200.035.138895
Produced by FastQC (version 0.11.5)