##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546582_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2928545
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.398311448176482	31.0	31.0	33.0	30.0	34.0
2	31.757006636401353	31.0	31.0	34.0	30.0	34.0
3	31.814300616859224	31.0	31.0	34.0	30.0	34.0
4	35.60695942865826	37.0	35.0	37.0	33.0	37.0
5	35.54871480547507	37.0	35.0	37.0	33.0	37.0
6	35.590770502075266	37.0	35.0	37.0	33.0	37.0
7	35.97363127423345	37.0	35.0	37.0	35.0	37.0
8	34.65162836835357	37.0	35.0	37.0	33.0	37.0
9	36.91242613652855	39.0	37.0	39.0	33.0	39.0
10	36.85255749868962	39.0	37.0	39.0	32.0	39.0
11	36.79785183427265	39.0	37.0	39.0	32.0	39.0
12	36.526612362111564	39.0	35.0	39.0	32.0	39.0
13	36.450587919939764	39.0	35.0	39.0	32.0	39.0
14	37.52333667401389	40.0	36.0	41.0	32.0	41.0
15	37.67502394533804	40.0	36.0	41.0	32.0	41.0
16	37.71298477571627	40.0	36.0	41.0	33.0	41.0
17	37.608141585667966	40.0	36.0	41.0	32.0	41.0
18	37.49745624533685	39.0	36.0	41.0	32.0	41.0
19	37.40277134208284	39.0	36.0	41.0	32.0	41.0
20	37.225757500738425	39.0	35.0	41.0	32.0	41.0
21	37.11302882489427	39.0	35.0	41.0	32.0	41.0
22	37.04879283056945	39.0	35.0	41.0	32.0	41.0
23	36.96606574254451	39.0	35.0	41.0	32.0	41.0
24	36.81694937246995	38.0	35.0	41.0	31.0	41.0
25	36.707918778779224	38.0	35.0	41.0	31.0	41.0
26	36.60396954801787	38.0	35.0	41.0	31.0	41.0
27	36.472262847250086	38.0	35.0	40.0	31.0	41.0
28	36.48055536110935	38.0	35.0	40.0	31.0	41.0
29	36.41658605211803	38.0	35.0	40.0	30.0	41.0
30	36.331428747039915	38.0	35.0	40.0	30.0	41.0
31	36.18612314306251	38.0	35.0	40.0	30.0	41.0
32	36.02309884259931	38.0	35.0	41.0	30.0	41.0
33	35.82322040467194	38.0	35.0	41.0	29.0	41.0
34	35.5737955196181	38.0	35.0	41.0	27.0	41.0
35	35.4295310469875	38.0	35.0	41.0	26.0	41.0
36	35.29395382348572	38.0	35.0	41.0	25.0	41.0
37	35.24457093881091	38.0	34.0	40.0	25.0	41.0
38	35.14289655784699	38.0	34.0	40.0	25.0	41.0
39	35.0636688184747	38.0	34.0	40.0	24.0	41.0
40	34.92058172232286	38.0	34.0	40.0	24.0	41.0
41	34.82721112361258	38.0	34.0	40.0	23.0	41.0
42	34.743231877946215	38.0	34.0	40.0	23.0	41.0
43	34.64641963842113	37.0	34.0	40.0	23.0	41.0
44	34.509491573460544	37.0	34.0	40.0	23.0	41.0
45	34.39638523567164	37.0	34.0	40.0	23.0	41.0
46	34.34237308970837	37.0	34.0	40.0	23.0	41.0
47	34.26826837217799	37.0	33.0	40.0	23.0	41.0
48	34.186511390468645	36.0	33.0	40.0	23.0	41.0
49	34.11280960340374	36.0	33.0	40.0	23.0	41.0
50	34.003842181014804	36.0	33.0	40.0	23.0	41.0
51	33.63875917904625	35.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	8.0
12	9.0
13	22.0
14	42.0
15	92.0
16	268.0
17	645.0
18	1439.0
19	3070.0
20	5029.0
21	8012.0
22	11791.0
23	17217.0
24	25723.0
25	38226.0
26	51281.0
27	59466.0
28	61434.0
29	64875.0
30	73285.0
31	85588.0
32	104525.0
33	132221.0
34	207449.0
35	298482.0
36	235361.0
37	300099.0
38	428677.0
39	713681.0
40	520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.3673735592248	23.062647150718192	26.194031507113603	16.375947782943406
2	35.66617552402302	23.74097034534214	26.905920858310182	13.686933272324653
3	29.65964326995146	24.266521429583634	32.512220232231364	13.561615068233543
4	25.65731446844764	26.676762692736496	31.841580033771038	15.824342805044825
5	22.610886976297103	30.888786069532827	30.93519136636111	15.565135587808962
6	20.593878530123323	39.20352939770432	29.499905243047316	10.702686829125044
7	82.59644294350949	4.378454147025229	10.698384351273415	2.3267185581918666
8	79.7375488510506	6.518663705013923	10.233306983502047	3.510480460433423
9	76.35778176534764	5.546713470341074	13.655006154933593	4.440498609377695
10	39.94837026578045	25.252403497299852	19.72955853504044	15.069667701879261
11	32.182773356735176	24.780052893160256	26.86491073212124	16.172263017983333
12	28.453651898809817	22.01011082295133	30.973299027332686	18.562938250906168
13	23.898966893115865	26.026576337396218	32.380140991516264	17.694315777971656
14	19.852827940154583	28.940890442182038	33.31674944383644	17.889532173826936
15	19.282988651361	23.21384851521831	39.86795490593452	17.635207927486174
16	22.090833502643804	21.74929871318351	36.9907582092814	19.169109574891287
17	21.95677375625097	22.173263514817084	31.603407152698693	24.266555576233248
18	23.796902557413325	22.765059099313824	33.47150205989664	19.966536283376215
19	26.479702377801946	24.695266762163463	29.039949872718363	19.785080987316228
20	27.165059782246814	24.314121859148486	28.113756148531095	20.407062210073605
21	25.131831677505385	25.69958802067238	30.692442834240214	18.476137467582024
22	24.166096133062666	22.926504458698773	29.921479779207765	22.9859196290308
23	22.4773052829989	26.03118613509439	29.22116614223104	22.270342439675677
24	22.209561403359007	24.12877384503226	33.29742244015373	20.364242311455005
25	22.445412312257453	24.4867331729579	31.224379341959914	21.843475172824732
26	20.266617040202558	26.65262101145791	30.17006738841302	22.91069455992652
27	20.716806468741304	25.664382824918174	31.19460346349467	22.42420724284585
28	20.476857961888925	25.948619536322642	33.03848839611479	20.536034105673636
29	21.888241430471446	25.125958453771414	31.446981350807313	21.53881876494983
30	23.13674538038514	24.712169353723436	31.306228861089725	20.8448564048017
31	26.073323100720664	24.65128587745792	28.197347146791323	21.07804387503009
32	25.858233354788812	24.949761741752305	28.235010901317892	20.956994002140995
33	25.4952544693696	24.56916318513118	27.848231801116253	22.08735054438296
34	21.46635957446445	26.894789050535334	30.09077886800442	21.548072506995794
35	22.05641367983077	26.494009823991092	29.287820402281678	22.161756093896457
36	25.257491348092653	26.720982603989352	27.66728870480051	20.354237343117486
37	22.91940195557862	28.173956691804293	28.342197234462844	20.56444411815424
38	22.674877797677688	28.60598010274727	27.456296556822586	21.26284554275246
39	22.45514410739804	27.082527330124684	27.56358532991639	22.89874323256088
40	23.718331116646663	24.828711868863206	29.092740593024864	22.360216421465267
41	21.116117389352052	24.438279077152647	29.122994524584733	25.322609008910568
42	23.236419450614555	25.724310194994445	27.72479166275403	23.31447869163697
43	22.645853145503995	25.270569514895623	28.5269647555356	23.55661258406478
44	21.981222757376102	26.662660126445044	28.600858105304855	22.755259010874003
45	21.189191219530517	27.221026140967613	28.14240518755901	23.44737745194286
46	23.00108074146035	26.58156183360679	28.367295021930683	22.050062403002173
47	22.036642769703043	26.13167972491459	29.31633285471113	22.51534465067124
48	22.65281906202568	25.525918160724864	29.721756025603156	22.099506751646295
49	22.876957670105806	24.56294849490105	29.656672511434863	22.903421323558284
50	21.58918507313359	26.163743428904112	29.661009135936105	22.586062362026194
51	21.469057159784125	26.882018203578912	28.27096732336365	23.377957313273313
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3133.0
1	4346.0
2	5559.0
3	28694.5
4	51830.0
5	35572.0
6	19314.0
7	19189.5
8	19065.0
9	19593.5
10	20122.0
11	20311.0
12	20500.0
13	20341.0
14	20182.0
15	19474.0
16	18766.0
17	18144.5
18	17523.0
19	16928.5
20	16334.0
21	16040.5
22	15747.0
23	16861.0
24	17975.0
25	19609.5
26	24107.0
27	26970.0
28	30122.5
29	33275.0
30	37430.0
31	41585.0
32	47177.5
33	52770.0
34	59913.0
35	67056.0
36	72140.5
37	77225.0
38	88787.0
39	100349.0
40	114856.5
41	129364.0
42	150556.0
43	171748.0
44	185677.0
45	199606.0
46	211868.5
47	224131.0
48	230706.0
49	237281.0
50	230693.5
51	224106.0
52	206109.5
53	188113.0
54	177049.5
55	165986.0
56	154609.5
57	143233.0
58	136013.0
59	128793.0
60	121113.5
61	113434.0
62	104543.5
63	95653.0
64	85471.5
65	75290.0
66	64713.5
67	54137.0
68	47182.5
69	40228.0
70	33647.5
71	27067.0
72	23141.5
73	19216.0
74	15548.0
75	9315.5
76	6751.0
77	5083.5
78	3416.0
79	2445.0
80	1474.0
81	1005.0
82	536.0
83	441.0
84	346.0
85	248.5
86	151.0
87	97.5
88	44.0
89	28.5
90	13.0
91	13.0
92	13.0
93	10.0
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2928545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.887085079513476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67602374440942	19.82903981724453
2	6.6979648842645805	3.333856438685725
3	2.2781148230595227	1.7008691226714938
4	1.2861582755417342	1.280349217165099
5	0.8776717973843209	1.0921346346696554
6	0.6490210934917066	0.9691345903276182
7	0.5303829118597939	0.923977925652235
8	0.4365282625983655	0.8691132808718178
9	0.36128981237663266	0.8092305269080845
>10	5.397902102976515	33.743786296594756
>50	1.6467284956139288	27.805676288123827
>100	0.15608609713107527	5.309182966776179
>500	0.004560148310620515	0.755622609719448
>1k	0.001425046347068911	0.4976129182846886
>5k	0.0	0.0
>10k+	1.425046347068911E-4	1.08041336630495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30464	1.040243533905062	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.829329923221258E-5	0.0	0.0	0.05975663682818601	0.0
2	6.829329923221258E-5	0.0	0.0	0.19668470178877223	0.0
3	6.829329923221258E-5	0.0	0.0	0.32364194506145544	0.0
4	6.829329923221258E-5	0.0	0.0	0.5097753321188508	0.0
5	6.829329923221258E-5	0.0	0.0	0.8763054690981358	0.0
6	6.829329923221258E-5	0.0	0.0	1.5409017105764125	0.0
7	6.829329923221258E-5	0.0	0.0	1.9307881558931141	0.0
8	1.0243994884831887E-4	0.0	0.0	2.8982651794662537	0.0
9	1.0243994884831887E-4	0.0	0.0	3.4352895379787576	0.0
10	1.0243994884831887E-4	0.0	0.0	4.192901253011308	0.0
11	1.0243994884831887E-4	0.0	0.0	4.680856876025467	0.0
12	1.0243994884831887E-4	0.0	0.0	5.1002118799608676	0.0
13	1.0243994884831887E-4	0.0	0.0	5.3062869103940695	0.0
14	1.0243994884831887E-4	0.0	0.0	5.405243900981546	0.0
15	1.0243994884831887E-4	0.0	0.0	5.504610651364414	0.0
16	1.0243994884831887E-4	0.0	0.0	5.696753848754245	0.0
17	1.0243994884831887E-4	0.0	0.0	5.8955556428192155	0.0
18	1.0243994884831887E-4	0.0	0.0	6.209704819287394	0.0
19	1.0243994884831887E-4	0.0	0.0	6.341032833710939	0.0
20	1.0243994884831887E-4	0.0	0.0	6.491380531970655	0.0
21	1.0243994884831887E-4	0.0	0.0	6.664947951969323	0.0
22	1.0243994884831887E-4	0.0	0.0	6.8531642846533005	0.0
23	1.0243994884831887E-4	0.0	0.0	7.05592709007374	0.0
24	1.0243994884831887E-4	0.0	0.0	7.224031046133831	0.0
25	1.0243994884831887E-4	0.0	0.0	7.37058846628616	0.0
26	1.0243994884831887E-4	0.0	0.0	7.5127068219883935	0.0
27	1.0243994884831887E-4	0.0	0.0	7.669508237025553	0.0
28	1.0243994884831887E-4	0.0	0.0	7.820231548431047	0.0
29	1.0243994884831887E-4	0.0	0.0	7.99455019472127	0.0
30	1.0243994884831887E-4	0.0	0.0	8.217493670064828	0.0
31	1.3658659846442516E-4	0.0	0.0	8.398880672825584	0.0
32	1.3658659846442516E-4	0.0	0.0	8.57948230264517	0.0
33	1.3658659846442516E-4	0.0	0.0	8.762098584792106	0.0
34	1.7073324808053147E-4	0.0	0.0	8.960763792258613	0.0
35	1.7073324808053147E-4	0.0	0.0	9.169741287909183	0.0
36	1.7073324808053147E-4	0.0	0.0	9.358947873432028	0.0
37	1.7073324808053147E-4	0.0	0.0	9.55081789762493	0.0
38	1.7073324808053147E-4	0.0	0.0	9.779054103658984	0.0
39	1.7073324808053147E-4	0.0	0.0	10.097164291482631	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18360	0.0	42.132355	1
TACGGGA	685	0.0	41.386864	4
CGTTCGG	795	0.0	39.905663	45
GCGATAT	265	0.0	39.056602	9
GGGCGAT	6105	0.0	38.144962	7
CGGTCTA	250	0.0	37.800003	31
AGGGCGA	3070	0.0	37.52443	6
ACGGGAT	610	0.0	37.2541	5
GGCGATT	1620	0.0	37.22222	8
AGGGTAC	1240	0.0	36.471775	6
TTACGGG	715	0.0	36.188812	3
GGCGATA	1215	0.0	36.11111	8
TACGAAC	25	0.0021076398	36.0	34
GCGTAAG	150	0.0	36.0	1
GTACGGG	945	0.0	35.95238	3
CTCACGA	265	0.0	35.660378	24
CGTAAGG	330	0.0	35.454544	2
TAAGGGA	2090	0.0	35.20335	4
TAGCGGG	1555	0.0	35.160774	3
TAGGGAC	1620	0.0	35.13889	5
>>END_MODULE