FastQCFastQC Report
Thu 26 May 2016
SRR1546581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546581_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1328796
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75600.5689360895126114No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG47240.35550979984888575No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC46870.35272532427851977No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC26360.19837507036445023No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23440.17640029018750808No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC18370.13824544926384486No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT15090.11356144961303316No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT14980.11273363255157301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG850.045.0000041
CGCACAT253.8911356E-545.028
TAAATCG207.0336845E-445.035
TTCGACT207.0336845E-445.027
CGGTCTA2100.043.9285731
CGTAAGG2300.042.065222
CGTTTTT50450.041.432111
GCGTAAG1200.041.2500041
CACGACG2250.040.026
ATAGGGT4050.039.4444474
ATAGACG403.458772E-739.3751
TCACGAC2450.038.5714325
CTCACGA2450.038.5714324
TAATCGG356.249027E-638.5714262
TACGGGT1000.038.254
TAAGGGA9150.038.114754
GCGCGAC4800.037.9687549
GATGAAT10550.037.96208620
CGATGAA10300.037.7912619
ACCCGCT4600.037.66304434